Bioaugmented hydrogen production from carboxymethyl cellulose and partially delignified corn stalks using isolated cultures

Bioconversion of wheat stalk to hydrogen by dark fermentation: Effect of different mixed microflora on hydrogen yield and cellulose solubilisation

“…Finally, the bottles were filled up to 150 mL with nutrient solution and flushed with a mixture of 80% N 2 and 20% CO 2 to maintain the proper pH and anaerobic conditions. Each liter of the nutrient solution contained beef extract, 2.0 g; yeast extract, 1.0 g; peptone, 4.0 g; MgSO 4 Á7H 2 O, 1.0 g; FeSO 4 Á7H 2 O, 0.1 g; NaCl, 3.0 g; KH 2 PO 4 , 1.0 g; K 2 HPO 4 , 1.0 g; l-cysteine 0.5 g (Ren et al, 2008). The bottles were kept at 35°C in a temperature controlled water-bath and inverted twice per day manually.…”

Hydrogen production from proteins via electrohydrogenesis in microbial electrolysis cells

“…sucrose, lactose) or polysaccharides (e.g. starch, cellulose, hemicellulose) as substrates for fermentative bacteria such as Clostridium and Ethanoligenens (Li et al, 2008;Ren et al, 2008;Xing et al, 2008). Dark-fermentation products (organic acids) can be utilized by purple non-sulfur bacteria in a photo-fermentation process to further evolve H 2 under nitrogendeficient and illuminated conditions (Hallenbeck and Benemann, 2002).…”

Membrane recovery of hydrogen from gaseous mixtures of biogenic and technogenic origin

“…Availability of amorphous cellulose to bacterial enzyme complexes after pretreatments increases significantly [35]. The various options for the biomass pretreatment which are most common currently applied described elsewhere [25,36,37]. For instance, the hydrolyzate of miscanthus used as a substrate for cellulolytic microorganism Caldicellulosirupter saccharolyticus and Thermotoga neapolitana raised the hydrogen yield to 74% and 85% of theoretically possible one (4 mol of H 2 per 1 mol of glucose or 498 ml H 2 per 1 g of glucose), respectively [28].…”