Bioaugmentation with the nicotine-degrading bacterium Pseudomonas sp. HF-1 in a sequencing batch reactor treating tobacco wastewater: Degradation study and analysis of its mechanisms

Wang, Meizhen; Yang, Guiqin; Huang, Min; Lv, Zhenmei; Jia, Xiao‐Bin

doi:10.1016/j.watres.2009.07.012

Cited by 72 publications

(49 citation statements)

References 43 publications

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“…Most bioaugmentation studies on wastewater have revealed that the inoculated strain(s) may significantly alter indigenous microbial community composition (Bai et al, 2011;Shi et al, 2015;Wang et al, 2009;Zhu et al, 2015). In our study, however, the indigenous microbial community maintained their predominant structure after the introduction of strain QJX1.…”

Section: Impact Of Bioaugmentation On Microbial Community In the Sandcontrasting

confidence: 55%

Treatment of groundwater containing Mn(II), Fe(II), As(III) and Sb(III) by bioaugmented quartz-sand filters

et al. 2016

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a b s t r a c tHigh concentrations of iron (Fe(II)) and manganese (Mn(II)) often occur simultaneously in groundwater. Previously, we demonstrated that Fe(II) and Mn(II) could be oxidized to biogenic Fe-Mn oxides (BFMO) via aeration and microbial oxidation, and the formed BFMO could further oxidize and adsorb other pollutants (e.g., arsenic (As(III)) and antimony (Sb(III))). To apply this finding to groundwater remediation, we established four quartz-sand columns for treating groundwater containing Fe(II), Mn(II), As(III), and Sb(III). A Mn-oxidizing bacterium (Pseudomonas sp. QJX-1) was inoculated into two parallel bioaugmented columns. Long-term treatment (120 d) showed that bioaugmentation accelerated the formation of Fe-Mn oxides, resulting in an increase in As and Sb removal. The bioaugmented columns also exhibited higher overall treatment effect and anti-shock load capacity than that of the nonbioaugmented columns. To clarify the causal relationship between the microbial community and treatment effect, we compared the biomass of active bacteria (reverse-transcribed real-time PCR), bacterial community composition (Miseq 16S rRNA sequencing) and community function (metagenomic sequencing) between the bioaugmented and non-bioaugmented columns. Results indicated that the QJX1 strain grew steadily and attached onto the filter material surface in the bioaugmented columns. In general, the inoculated strain did not significantly alter the composition of the indigenous bacterial community, but did improve the relative abundances of xenobiotic metabolism genes and Mn oxidation gene. Thus, bioaugmentation intensified microbial degradation/utilization for the direct removal of pollutants and increased the formation of Fe-Mn oxides for the indirect removal of pollutants. Our study provides an alternative method for the treatment of groundwater containing high Fe(II), Mn(II) and As/ Sb.

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Section: Impact Of Bioaugmentation On Microbial Community In the Sandcontrasting

confidence: 55%

Treatment of groundwater containing Mn(II), Fe(II), As(III) and Sb(III) by bioaugmented quartz-sand filters

et al. 2016

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“…Because wastewater provides a number of potential nitrogen sources, we added either NH 4 Cl, NaNO 3 , or NaNO 2 to the casein medium. Again, we detected LasR Ϫ mutant cheaters under all conditions (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Because of this potential instability, there has been substantial effort in determining the factors that are involved in the loss of the bioaugmented strain in cultures (4). Most of the prior work has focused on the competition between inoculated bacteria and indigenous bacterial communities.…”

mentioning

confidence: 99%

Nitrogen Source Stabilization of Quorum Sensing in the Pseudomonas aeruginosa Bioaugmentation Strain SD-1

Wang

Lai

Dandekar

et al. 2017

Appl Environ Microbiol

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Pseudomonas aeruginosa SD-1 is efficient at degrading aromatic compounds and can therefore contribute to the bioremediation of wastewater. P. aeruginosa uses quorum sensing (QS) to regulate the production of numerous secreted "public goods." In wastewater bioaugmentation applications, there are myriad nitrogen sources, and we queried whether various nitrogen sources impact the stabilities of both QS and the bacterial populations. In a laboratory strain of P. aeruginosa, PAO1, the absence of a nitrogen source has been shown to destabilize these populations through the emergence of QS mutant "cheaters." We tested the ability of SD-1 to grow in casein broth, a condition that requires QS for growth, when the nitrogen source with either NH 4 Cl, NaNO 3 , or NaNO 2 or with no added nitrogen source. There was great variability in susceptibility to invasion by QS mutant cheaters and, by extension, the stability of the SD-1 population. When grown with NH 4 Cl as an extra nitrogen source, no population collapse was observed; by contrast, twothirds of cultures grown in the presence of NaNO 2 collapsed. In the populations that collapsed, the frequency of social cheaters exceeded 40%. NaNO 3 and NaNO 2 directly favor QS mutants of P. aeruginosa SD-1. Although the mechanism by which these nitrogen sources act is not clear, these data indicate that the metabolism of nitrogen can affect the stability of bacterial populations, an important observation for continuing industrial applications with this species.IMPORTANCE Bioaugmentation as a method to help remediate wastewater pollutant streams holds significant potential to enhance traditional methods of treatment. Addition of microbes that can catabolize organic pollutants can be an effective method to remove several toxic compounds. Such bioaugmented strains of bacteria have been shown to be susceptible to competition from the microbiota that are present in wastewater streams, limiting their potential effectiveness. Here, we show that bioaugmentation strains of bacteria might also be susceptible to invasion by social cheaters and that the nitrogen sources available in the wastewater might influence the ability of cheaters to overtake the bioaugmentation strains. Our results imply that control over the nitrogen sources in a wastewater stream or selective addition of certain nitrogen sources could help stabilize bioaugmentation strains of bacteria.KEYWORDS quorum sensing, social cheating, bioaugmentation, long-term stabilization, inorganic nitrogen B ioaugmentation, the use of selected microbial strains in activated sludge to strengthen their abilities for pollutant removal (1), has significant advantages in the degradation of toxic and hazardous compounds. These advantages include cost and

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“…Entre as tecnologias utilizadas para fazer a remoção de nicotina em solução aquosa pode-se citar a biodegradação (Wang et al, 2009), sistema de tratamento de água seguido de ozonização (Huerta-Fontela et al, 2008b), além do processo de Foto-Fenton (Klamerth et al, 2010;Rodriguez et al, 2011). Vê-se, portanto, que Processos Oxidativos Avançados (POAs) têm sido considerados promissores para a remediação de águas e efluentes.…”

Section: Introductionunclassified

DEGRADAÇÃO FOTOCATALÍTICA DE NICOTINA EM SOLUÇÃO AQUOSA EMPREGANDO ZnO e TiO2 COMERCIAIS E CATALISADORES NÃO CONVENCIONAIS SUPORTADOS EM SÍLICA

Franco¹,

Silva²,

Santos³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -A fotocatálise heterogênea foi aplicada para promover a degradação da nicotina, sob radiação UV, empregando ZnO e TiO 2 comerciais e 5 catalisadores não convencionais preparados a partir de resíduos químicos (banhos galvânicos, resíduo de laboratório e resíduo de catalisador Ziegler-Natta proveniente de uma indústria petroquímica. Dois delineamentos com composto central rotacional foram realizados para os catalisadores comerciais a fim de estabelecer a influência da concentração de catalisador, pH da solução e concentração inicial do contaminante. As condições nas quais a velocidade de reação é máxima foram usadas para testar os catalisadores não convencionais. Verificou-se que, nas condições otimizadas, o ZnO chega a degradação total em um hora, enquanto que com TiO 2 atinge-se aproximadamente 90%. Os catalisadores não convencionais foram caracterizados por RBS, DRS-UV e área BET. O catalisador sintetizado a partir de resíduo de uma indústria petroquímica apresentou a maior área superficial e a menor energia de band gap, e exibiu a maior degradação (43%).

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Bioaugmentation with the nicotine-degrading bacterium Pseudomonas sp. HF-1 in a sequencing batch reactor treating tobacco wastewater: Degradation study and analysis of its mechanisms

Cited by 72 publications

References 43 publications

Treatment of groundwater containing Mn(II), Fe(II), As(III) and Sb(III) by bioaugmented quartz-sand filters

Treatment of groundwater containing Mn(II), Fe(II), As(III) and Sb(III) by bioaugmented quartz-sand filters

Nitrogen Source Stabilization of Quorum Sensing in the Pseudomonas aeruginosa Bioaugmentation Strain SD-1

DEGRADAÇÃO FOTOCATALÍTICA DE NICOTINA EM SOLUÇÃO AQUOSA EMPREGANDO ZnO e TiO2 COMERCIAIS E CATALISADORES NÃO CONVENCIONAIS SUPORTADOS EM SÍLICA

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