BIOATIVIDADE ALELOPÁTICA DE EXTRATOS METANÓLICOS DE Myriophyllum aquaticum (Vell.) Verdc. SOBRE Microcystis aeruginosa Kutzing

Kitamura, Rafael Shinji Akiyama; Silva, Ana Roberta; Pagioro, Thomaz Aurélio; Martins, Lúcia Regina Rocha

doi:10.18677/encibio_2021c10

Cited by 2 publications

(6 citation statements)

References 18 publications

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“…In a review of the interactions among compounds with allelopathic properties produced by macrophytes and cyanobacteria, Mohamed (2017) explained that in addition to controlling cell density, the macrophytes had the potential for phytoaccumulation and phytotransformation of cyanotoxins with potential metabolization and reduced toxicity, indicating that these species can be used to mitigate the impacts of harmful algal blooms. Besides, it is known that methanolic extracts of M. aquaticum corroborate to reduce microcystin-LR concentration and M. aeruginosa cell growth (Kitamura et al, 2021). In addition to the possible capacity of phytoremediation, the present results of microcystin-LR produced per cell demonstrated that allelopathic mechanisms could interfere in the production of cyanotoxins by cyanobacteria and more studies should be conducted to understand the possible mechanisms involved.…”

Section: Effects On the Concentration Of Intracellular Microcystin-lrmentioning

confidence: 54%

“…Moreover, these plant species are important to maintain the quality of lakes since they compete with phytoplankton for light and nutrients, reduce suspended sediments, and contribute to water purification (Li et al, 2021). So, these techniques can be considered an efficient nature-based solution (NBS) that can corroborate to mitigate the impact of harmful algae blooms (Kitamura et al, 2021;Zhu et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Use of Myriophyllum aquaticum to inhibit Microcystis aeruginosa growth and remove microcystin-LR

Kitamura

Silva²,

Pagioro

et al. 2022

Rev. Bras. Ciênc. Ambient.

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Harmful algal blooms are one of the greatest challenges when preserving water sources, especially when involving cyanobacteria such as Microcystis aeruginosa. Finding remediation possibilities is needed, and one of them has been the use of macrophytes such as the species Myriophyllum, which have presented allelopathic mechanisms of phytoplankton control. Thus, this work aimed to evaluate the inhibition of M. aeruginosa cell growth in a co-exposure with Myriophyllum aquaticum and the influence on microcystin-LR concentration. The experiments were carried out using a culture of M. aeruginosa (1x106 cells mL-1) in a co-exposure with M. aquaticum for seven days. The inhibitory effects were investigated by counting the cells; the effects on photosynthetic pigments were measured and microcystin-LR was quantified in the culture medium on the last experimental day. To evaluate the possible effects of competition for nutrients and space, the concentration of total orthophosphate was quantified and treatment with plastic plants was used. The experiments with Myriophyllum aquaticum achieved the total inhibition of M. aeruginosa growth and a significant reduction of the photosynthetic pigments (> 98%). Additionally, we observed a reduction of microcystin-LR concentration (79%) in the tests with macrophytes when compared to the control. Competition for space and nutrients was not observed, demonstrating that the effects on M. aeruginosa were caused by aquatic macrophyte presence. These results may indicate that M. aquaticum causes inhibitory effects on cyanobacteria growth by allelopathic effects and removes microcystin-LR.

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Section: Effects On the Concentration Of Intracellular Microcystin-lrmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Use of Myriophyllum aquaticum to inhibit Microcystis aeruginosa growth and remove microcystin-LR

Kitamura

Silva²,

Pagioro

et al. 2022

Rev. Bras. Ciênc. Ambient.

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“…The liquid extract was frozen at −80 • C and lyophilized (Liotop, Brazil). The lyophilized extract was stored at −20 • C, until the preparation of the solutions for the experiments to evaluate allelopathic activity to control the cyanobacteria [20].…”

Section: Preparation and Obtaining Of Aqueous Extractsmentioning

confidence: 99%

“…The cultures were maintained under controlled conditions of temperature, light, and photoperiod as described previously. After 10 days of incubation, the cell concentration was measured to determine if the extracts had an algicidal (growth inhibition) or algistatic effect (growth after treatment in a new culture medium without allelopathic compounds) [20].…”

Section: Evaluation Of Cellular Viabilitymentioning

confidence: 99%

“…In this context, submerged aquatic macrophytes contribute to the maintenance of water bodies, mainly through local oxygenation, and the control of phytoplankton by releasing allelochemicals into the aquatic environment to reduce competition for light, carbon, and nutrients [9,15,17,18]. Among the species of submerged aquatic macrophytes, the genus Myriophyllum has been studied due to its allelopathic capacity in cyanobacteria, mainly through the production of allelochemicals [20][21][22][23][24]. The major class with an inhibitory potential on cyanobacteria is phenolic compounds [9,25,26].…”

Section: Introductionmentioning

confidence: 99%

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Enhancing Biocontrol of Harmful Algae Blooms: Seasonal Variation in Allelopathic Capacity of Myriophyllum aquaticum

Kitamura

Silva²,

Pagioro

et al. 2023

Water

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Myriophyllum aquaticum has shown potential allelopathic effects for the biocontrol of cyanobacteria and cyanotoxins. However, the composition of allelochemicals and their biological effects may be influenced by seasonal changes. In this study, we investigated the impact of aqueous extracts of M. aquaticum collected in different seasons on the growth of Microcystis aeruginosa and the concentration of microcystin-LR. Plant samples were extracted using ultrasound cycles in aqueous solutions, and extracts at varying concentrations (0.1, 10, and 100 mg/L) and a control treatment were inoculated with M. aeruginosa, and cell growth was analyzed using a Neubauer chamber. Photosynthetic pigment quantification was used to measure physiological effects and liquid chromatography was used to evaluate the microcystin-LR concentrations. The extracts of plants collected during autumn and winter exhibited higher inhibition of M. aeruginosa growth and a reduction in photosynthetic pigments compared to those collected during spring and summer. These results can be explained by the higher presence of phenolic compounds in the composition of extracts from autumn and winter. Microcystin-LR concentrations were decreased at 10 and 100 mg/L, with the highest efficiency observed in autumn, while spring showed lower efficiency. Our findings suggest that M. aquaticum extracts have inhibitory potential on M. aeruginosa, particularly during the autumn season, making them a promising nature-based solution for the biocontrol of harmful algal blooms.

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BIOATIVIDADE ALELOPÁTICA DE EXTRATOS METANÓLICOS DE Myriophyllum aquaticum (Vell.) Verdc. SOBRE Microcystis aeruginosa Kutzing

Cited by 2 publications

References 18 publications

Use of Myriophyllum aquaticum to inhibit Microcystis aeruginosa growth and remove microcystin-LR

Use of Myriophyllum aquaticum to inhibit Microcystis aeruginosa growth and remove microcystin-LR

Enhancing Biocontrol of Harmful Algae Blooms: Seasonal Variation in Allelopathic Capacity of Myriophyllum aquaticum

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