Bioassays for Bioactivity Screening

Montalvão, Sofia; Singh, Vineeta; Haque, Shafiul

doi:10.1016/b978-0-444-63359-0.00005-7

Cited by 6 publications

(2 citation statements)

References 64 publications

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“…The phytochemical analysis of the L. philadelphicum flower revealed the presence of flavonoids, tannins, glycosides, phenols, coumarins, and terpenoids (Table 1). Several bioactive constituents have been reported from plants such as Azardirachta indica [19], Senna alata [20], and Terminalia bellerica [21]. In their efforts to search for potential antimicrobial compounds, Dontha et al [22] reported the isolation of active constituents of Ixora Javanica DC flower extract and its phytochemical characterization.…”

Section: Antimicrobial and Phytochemical Screening Of Solvent Extractsmentioning

confidence: 99%

Lilium philadelphicum Flower as a Novel Source of Antimicrobial Agents: A Study of Bioactivity, Phytochemical Analysis, and Partial Identification of Antimicrobial Metabolites

Singh

Alhazmi

et al. 2021

Sustainability

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The members of the Liliaceae family are considered an excellent source of biologically active compounds. However, work on antimicrobial potential and characterization of the bioactive fractions of the Lilium philadelphicum flower is limited and needs to be explored. The present study reports the antimicrobial potential of the bioactive fraction extracted from the flower of L. philadelphicum (red lily) and partial characterization of the bioactive compound(s). The antimicrobial activity was tested against nine different Gram-positive and Gram-negative bacterial strains. The minimum inhibitory concentration (MIC) values of methanolic extract of the L. philadelphicum flower against Acinetobacter bouvetii, Achromobacter xylosoxidans, Bacillus subtilis MTCC 121, Candida albicans MTCC 183, Klebsiella pneumoniae MTCC 3384, and Salmonella typhi MTCC 537 were 25, 50, 12.5, 50, 100, and 50 μg/mL, respectively. The phytochemical analysis of the extract revealed the presence of phenols, flavonoids, tannins, terpenoids, glycosides, coumarins, and quinones. The cytotoxicity of the partially purified compound against the HepG2 cell line using MTT assay demonstrated up to 90% cell viability with a bioactive compound concentration of 50 μg/mL. However, the increase in the bioactive compound’s concentration up to 1000 μg/mL resulted in nearly 80% cell viability. This minor decline in cell viability suggests the importance and suitability of the bioactive compound for therapeutic applications. Spectroscopic studies of the bioactive compound by UV-visible spectroscopy, FT-infrared spectroscopy, gas chromatography-mass spectrometry (GC-MS), as well as phytochemical analysis, suggested the presence of a terpenoid moiety, which may be responsible for the antimicrobial property of the L. philadelphicum flower.

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Section: Antimicrobial and Phytochemical Screening Of Solvent Extractsmentioning

confidence: 99%

Lilium philadelphicum Flower as a Novel Source of Antimicrobial Agents: A Study of Bioactivity, Phytochemical Analysis, and Partial Identification of Antimicrobial Metabolites

Singh

Alhazmi

et al. 2021

Sustainability

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“…The antibacterial activity was evaluated using the Disc Diffusion method (Montalvão, Singh & Haque, 2014), according to the Clinical and Laboratory Standards Institute (CLSI, 2017). MRSA was swabed on to Muller-Hinton agar medium (Sigma-Aldrich, St. Louis, MO, USA) with the total concentration of 0.5 Mc Farland standart.…”

Section: Antibacterial Assaymentioning

confidence: 99%

Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

Kristiana

Bedoux

Pals

et al. 2020

PeerJ

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The aims of this work are to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-Herpes simplex virus type 1 (HSV-1) activities of bio compounds. A total of 15 species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated two active bacteria. Ethyl acetate extracts from supernatants, indicating extracelullar compounds, showed an inhibition zone against MRSA at concentrations of 500-1,000 µg/ml. DNA sequence analysis showed that the strain KJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, whereas the strain NP31-01 isolated from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of Pseudoalteromonas rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5-1,000 µg/ml. No anti HSV-1 was observed for both isolated bacteria. This is the first study describing research on anti-MRSA, cytotoxicity and anti HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced by Pseudoalteromonas rubra and V. salarius, had potential anti-MRSA activity. However, only extracts from Pseudoalteromonas rubra showed cytotoxic effects on Vero cells. Three compounds were identified by LC/MS after purification from culture supernatant. activity of marine bacteria associated with sponges from the waters off the coast of South East India. Microbiological Research 161 (3):252-262 DOI 10.1016/j.micres.2005.09.002. Avila C. 2006. Molluscan natural products as biological models: chemical ecology, histology, and laboratory culture. Chand S, Karuso P. 2017. Isolation and total synthesis of two novel metabolites from the fissurellid mollusc Scutus antipodes. Tetrahedron Letters 58(10):P. 1992. A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification. Research in Microbiology 143(8):785-790

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Bioactive Metabolites Isolated from Microorganisms for Healthcare: Types and Delivery Routes

Mohanta

Maneesha

Ghangal

et al. 2019

Environmental Chemistry for a Sustainable World

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Bioassays for Bioactivity Screening

Cited by 6 publications

References 64 publications

Lilium philadelphicum Flower as a Novel Source of Antimicrobial Agents: A Study of Bioactivity, Phytochemical Analysis, and Partial Identification of Antimicrobial Metabolites

Lilium philadelphicum Flower as a Novel Source of Antimicrobial Agents: A Study of Bioactivity, Phytochemical Analysis, and Partial Identification of Antimicrobial Metabolites

Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

Bioactive Metabolites Isolated from Microorganisms for Healthcare: Types and Delivery Routes

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