Bioassays for Anticancer Activities

McCauley, Janice I.; Zivanovic, Ana; Skropeta, Danielle

doi:10.1007/978-1-62703-577-4_14

Cited by 96 publications

(62 citation statements)

References 27 publications

(36 reference statements)

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“…18 Standard cancer cell cytotoxicity bioassays were conducted using HCT-116 human colon carcinoma applying the common MTT-based methods well documented in the literature. 19 …”

Section: Methodsmentioning

confidence: 99%

Marinocyanins, cytotoxic bromo-phenazinone meroterpenoids from a marine bacterium from the streptomycete clade MAR4

et al. 2017

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Six cytotoxic and antimicrobial metabolites of a new bromo-phenazinone class, the marinocyanins A-F (1–6), were isolated together with the known bacterial metabolites 2-bromo-1-hydroxyphenazine (7), lavanducyanin (8, WS-9659A) and its chlorinated analog WS-9659B (9). These metabolites were purified by bioassay-guided fractionation of the extracts of our MAR4 marine actinomycete strains CNS-284 and CNY-960. The structures of the new compounds were determined by detailed spectroscopic methods and marinocyanin A (1) was confirmed by crystallographic methods. The marinocyanins represent the first bromo-phenazinones with an N-isoprenoid substituent in the skeleton. Marinocyanins A-F show strong to weak cytotoxicity against HCT-116 human colon carcinoma and possess modest antimicrobial activities against Staphylococcus aureus and amphotericin-resistant Candida albicans.

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“…18 Standard cancer cell cytotoxicity bioassays were conducted using HCT-116 human colon carcinoma applying the common MTT-based methods well documented in the literature. 19 …”

Section: Methodsmentioning

confidence: 99%

Marinocyanins, cytotoxic bromo-phenazinone meroterpenoids from a marine bacterium from the streptomycete clade MAR4

et al. 2017

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“…Around a world, big number of people are diagnosed with cancer each year, and most of a patients die from it [14]. Several strategies are being developed to fight against cancer [15][16][17]. Cis-Pt(II) complex is the first number of platinum-containing anti-cancer drug [18].…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Spectral and Biological Studies of Ni(II), Pd(II), and Pt(IV) Complexes with New Heterocyclic ligand Derived from Azo-Schiff Bases Dye

Al-Adilee¹,

Dakheel²

2018

Eurasian J Anal Chem

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New heterocyclic azo-schiff base dye (L2) have been synthesized from a reaction of 4-(dimethylamino)benzaldehyde with (E)-5-((1H-benzo[d]imidazol-2-yl) diazenyl) -2amine-4,6-dimethylpyridin (L1) . A new class of Ni(II), Pd(II), and Pt(IV) complexes of azo-schiff base of the type [Ni(L2)2Cl2], [Pd(L2) Cl2] , and [Pt-2amine-4,6-dimethylpyridin. Both ligand and its metal complexes were characterized by the spectroscopic and analytical methods such as mass spectral, 1 H-NMR, FT-IR, UV-Visb.,X-ray, TGA and SEM, elemental analysis, molar conductance measurements and magnetic susceptibility., spectral studies suggest the mole ratio [M:L] was [1:2] for Ni(II) and Pt(IV) metal ions, but [1:1] for Pd(II) metal ion. The structures of theses complexes were elucidated on the basis of different techniques suggest the structures of the prepared metal complexes octahedral geometry for the Ni(II) and Pt(IV) complexes and square planar geometry for the Pd(II) complex. The antibacterial activity of the ligand and prepared metal complexes was also studied against gram positive Staphylococcus aureus and gram negative bacteria Escherichia coli, the antifungal activity of the azo-schiff base and metal complexes against the fungi Alternaria. It is found that some of the complexes are quite effective against fungi Alternaria. In this study the cytotoxicity of Pd(II)-complex on human (PC3) cancer and normal cells were studied using MTT assay. Pd(II)-complex showed selective cytotoxicity against cancer cell line with IC50 = 450 μg/ml, while it was very safe on normal cells line with IC50 = 14554885 μg /ml, respectively for human cells .The results indicate undoubtedly the possibility of using them as antitumor drugs in the field of medicine and Pharmacy against prostate cancer.

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“…The plates were read at 595 nm on a scanning multi‐well spectrophotometer. The cell viability (%) was calculated using the test and control optical density values (McCauley, Zivanovic, & Skropeta, ).…”

Section: Methodsmentioning

confidence: 99%

“…The cell viability (%) was calculated using the test and control optical density values (McCauley, Zivanovic, & Skropeta, 2013).…”

Section: F I G U R E 1 Preparation Of Plant Extract (A) Curd (B) Anmentioning

confidence: 99%

Preparation of herbal curd with Gymnema sylvestre and its characterization for the treatment of liver cancer

Jini

Arumugam

Arivarasu

et al. 2019

J Food Process Engineering

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Herbal curd was prepared by adding the Gymnema sylvestre leaf extract during fermentation and its activity against liver cancer was investigated on HepG2 cell lines by MTT (3‐[4,5‐dimethylthiazole‐2yl]‐2,5‐diphenyltetrazolium bromide) assay. The prepared herbal curd was characterized with the help of qualitative phytochemical analysis, Fourier transform infrared (FT‐IR) spectroscopy and gas chromatography‐ mass spectrometry (GC–MS). The sensory attributes and stability of the herbal curd were also determined. The qualitative phytochemical analysis of the herbal curd showed the transfer of phenolic compounds from the plant extract to herbal curd. The FT‐IR analysis exhibited that the Anhydride group present in the plant extract was infused into the herbal curd in addition to its other functional groups such as Alcohol, Alkyne, Alkene, Amide, Sulfate, Ether and Esters. The GC–MS analysis revealed the presence of 10 new compounds which were not present in curd or plant extract. The results of MTT assay showed that the herbal curd had significantly reduced the growth of HepG2 cells. The sensory evaluation proved that the herbal curd was delicious than the plant extract. Hence, it can be used as a potential food supplement for the treatment of liver cancer. Practical Applications Fermented milk products have been used for the prevention and treatment of various diseases including cancer and diabetes. Recently, the usage of medicinal plants for the treatment of cancer and other diseases has gained incredible attention due to their higher activity and minimal side effects. Gymnema sylvestre is a pharmacologically significant medicinal plant, used in different kinds of poly‐herbal formulations for the treatment of various diseases. In the present study, it was used for the preparation of herbal curd that will serve as an alternative remedial measure for treating liver cancer.

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Bioassays for Anticancer Activities

Cited by 96 publications

References 27 publications

Marinocyanins, cytotoxic bromo-phenazinone meroterpenoids from a marine bacterium from the streptomycete clade MAR4

Marinocyanins, cytotoxic bromo-phenazinone meroterpenoids from a marine bacterium from the streptomycete clade MAR4

Synthesis, Spectral and Biological Studies of Ni(II), Pd(II), and Pt(IV) Complexes with New Heterocyclic ligand Derived from Azo-Schiff Bases Dye

Preparation of herbal curd with Gymnema sylvestre and its characterization for the treatment of liver cancer

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