Bioassay of prostacyclin and endothelium‐derived relaxing factor (EDRF) from porcine aortic endothelial cells

Gryglewski, Ryszard J.; Moncada, Salvador; Palmer, Richard

doi:10.1111/j.1476-5381.1986.tb14586.x

Cited by 217 publications

(83 citation statements)

References 31 publications

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“…Release ofEDRF and nitric oxidefrom endothelial cells EDRF was released by bradykinin (Bk) from porcine aortic endothelial cells cultured on microcarriers and bioassayed either on spiral strips of rabbit thoracic aorta or on platelet aggregation as described previously (Gryglewski et al, 1986a;Radomski et al, 1987). Briefly, between 0.5 and 1.5 ml of microcarriers (0.8-5.0 x 10' cells) in a total volume of 2 ml were stimulated with Bk (1-100 nM).…”

Section: Methodsmentioning

confidence: 99%

The anti‐aggregating properties of vascular endothelium: interactions between prostacyclin and nitric oxide

Radomski

Palmer

Moncada

1987

British J Pharmacology

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1 The interactions between endothelium-derived nitric oxide (NO) and prostacyclin as inhibitors of platelet aggregation were examined. 2 Porcine aortic endothelial cells treated with indomethacin and stimulated with bradykinin (10-100 nM) released NO in quantities sufficient to account for the inhibition of platelet aggregation attributed to endothelium-derived relaxing factor (EDRF). 3 In the absence of indomethacin, stimulation of the cells with bradykinin (1-3 nM) released small amounts of prostacyclin and EDRF which synergistically inhibited platelet aggregation. 4 EDRF and authentic NO also caused disaggregation of platelets aggregated either with collagen or with U46619. 5 A reciprocal potentiation of both the anti-and the dis-aggregating activity was also observed between low concentrations of prostacyclin and authentic NO or EDRF released from endothelial cells. 6 It is likely that interactions between prostacyclin and NO released by the endothelium play a role in the homeostatic regulation of platelet-vessel wall interactions.

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Section: Methodsmentioning

confidence: 99%

The anti‐aggregating properties of vascular endothelium: interactions between prostacyclin and nitric oxide

Radomski

Palmer

Moncada

1987

British J Pharmacology

Self Cite

1,080

461

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“…EDNO and PGI 2 may act the synergic way [30] as important anti-thrombotic factor in the atrium. Considering that cultured endothelial cells have shown changes in response to agonists as a result of receptor down-regulation [13,14], the proposed methodology can be more useful due to its easy execution, low cost, good reproducibility and effectiveness in mimicking in vivo conditions.…”

Section: Discussionmentioning

confidence: 99%

“…CHUA, YL ET AL -Adaptation of bioassay to detect endotheliumderived relaxing factors from the canine atrial endocardium Bras Cir Cardiovasc 2009; 24(2): 225-232 regulation of receptors when the cells are cultured and the response of the cultured endothelium to agonists can be altered [13,14]. In the present experiments, a bioassay technique was developed to detect luminal release of endothelium-derived relaxing factors from intact canine atrial endocardium.…”

mentioning

confidence: 99%

Adaptação de um sistema de ensaio biológico para detecção de fatores relaxantes endoteliais derivados do endocárdio atrial canino

Chua¹,

Évora²,

Celotto³

et al. 2009

Rev Bras Cir Cardiovasc

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Adaptação de um sistema de ensaio biológico para detecção de fatores relaxantes endoteliais derivados do endocárdio atrial caninoAdaptation of bioassay to detect endotheliumderived relaxing factors from the canine atrial endocardium Abstract Objective: The aim of this study was to assess the release of endothelium-derived relaxing factors from the endocardium of canine atrial appendage.Method: To study the release of endothelium-derived relaxing factor (EDRF) from intact atrial endocardial endothelium, tube-shaped sutures of canine atrial appendages were performed and effluents from these tubes were bioassayed (isolated perfused organ chamber system) for detection of EDRF in canine coronary artery.Results: Effluent from the right atrial appendage caused a relaxation of 58.4 + 10.1% and the left atrial appendage 74.9 + 8.5% from the initial prostagladin FF2α α α α α contraction in coronary artery. No significant statistical difference was detected in effluent from the right and left atrial appendages. This relaxation was abolished by treating the heart tubes with Triton X-100 and reduced by treatment with LNMMA, a competitive inhibitor of nitric oxide and with indomethacin, an inhibitor of the cyclo-oxygenase pathway, also indicating the release of vasodilatory prostanoids from the endocardial endothelium.Conclusion: This study showed for the first time, in vitro luminal release of EDRF and prostacyclin from the canine heart atrium. The ability of the endocardial endothelium to produce these factors could play an important role in preventing thrombus formation in the cardiac chambers. 226CHUA, YL ET AL -Adaptation of bioassay to detect endotheliumderived relaxing factors from the canine atrial endocardium Bras Cir Cardiovasc 2009; 24(2): 225-232 regulation of receptors when the cells are cultured and the response of the cultured endothelium to agonists can be altered [13,14]. In the present experiments, a bioassay technique was developed to detect luminal release of endothelium-derived relaxing factors from intact canine atrial endocardium. Rev METHODS Bioassay techniqueHeartworm free mongrel dogs (25-30 kg; n=10) of either sex were anesthetized with intravenous pentobarbital sodium (30 mg/kg bolus injection; Fort Dodge Laboratories) and exsanguinated via the carotid arteries; the beating heart was excised and immersed in cool, oxygenated physiological salt solution of the following millimolar composition: NaCl, 118.3; KCl, 4.7; MgSO 4 , 1.2; KH 2 PO 4 , 1.22; CaCl 2 , 2.5; NaHCO 3 , 25.0 and glucose, 11.1 (control solution). The procedures and the handling of the animals were reviewed and approved by the Institutional Animal Care and Use Committee of the Mayo Foundation.The left circumflex coronary artery was carefully dissected free from the heart and placed in control solution. A coronary ring of about 3 mm was cut and the endothelium removed with a pair of watchmakers forceps. This procedure effectively removes endothelium from the coronary ring without affecting the smooth muscle ability to contract or r...

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“…Moro, Darley-Usmar et al examined the role of PN on isolated rabbit aortic strips [60] denuded of endothelium and superfused in a cascade bioassay [61]. The addition of PN (30-300 M) to the superfusate resulted in concentration-dependent relaxations of all three detector strips and these observed relaxations were inhibited by oxyhemoglobin [61].…”

Section: Vasorelaxant Properties Of Peroxyni-tritementioning

confidence: 99%

Potential Benefits of Peroxynitrite

Nossaman

Kadowitz²

2008

TOPHARMJ

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Peroxynitrite (PN) is generated by the reaction of nitric oxide (NO) and superoxide in one of the most rapid reactions in biology. Studies have reported that PN is a cytotoxic molecule that contributes to vascular injury in a number of disease states. However, it has become apparent that PN has beneficial effects including vasodilation, inhibition of platelet aggregation, inhibition of inflammatory cell adhesion, and protection against ischemia/reperfusion injury in the heart. It is our hypothesis that PN may serve to inactivate superoxide and prolong the actions of NO in the circulation. This manuscript reviews the beneficial effects of PN in the cardiovascular system. Keywords:Nitric oxide/*metabolism, nitric oxide synthase/metabolism/physiology, peroxynitrous acid/metabolism, reactive nitrogen species/metabolism, reactive oxygen species/metabolism, endothelium, vascular/drug effects/metabolism, muscle, smooth, vascular/drug effects/*metabolism, vasodilator agents/adverse effects, oxidative stress/*physiology, vasodilation/drug effects.

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Bioassay of prostacyclin and endothelium‐derived relaxing factor (EDRF) from porcine aortic endothelial cells

Cited by 217 publications

References 31 publications

The anti‐aggregating properties of vascular endothelium: interactions between prostacyclin and nitric oxide

The anti‐aggregating properties of vascular endothelium: interactions between prostacyclin and nitric oxide

Adaptação de um sistema de ensaio biológico para detecção de fatores relaxantes endoteliais derivados do endocárdio atrial canino

Potential Benefits of Peroxynitrite

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