Bioassay‐guided separation and identification of the anticancer composition from <i>Curcuma longa</i> L. by the combination strategy of methanol gradient countercurrent chromatography and ultra‐high‐performance liquid chromatography coupled with high‐resolution mass spectrometry

Li, Lanjie; Yu, Yang; Lu, Dini; Chen, Jiang-xian; Guo, Jun; Liang, Junling; Zhang, Anling; Yang, Zhengtao

doi:10.1002/jssc.202200348

Cited by 5 publications

(3 citation statements)

References 50 publications

(69 reference statements)

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“…An appropriate two-phase solvent system plays a crucial role in the successful separation of targeted compounds in CCC, with the partition coefficient (K) being the most critical parameter for selecting the solvent system [21]. The determination of K values for the targeted compounds was performed through HPLC analysis using a previously established method [22][23][24].…”

Section: Solvent System Selection and Preparationmentioning

confidence: 99%

Separation of bufadienolides from Helleborus thibetanus Franch. by a combination approach involving macroporous resin column chromatography and gradient countercurrent chromatography

Guo,

Chen,

Zhang

et al. 2024

J of Separation Science

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In this study, a combination approach involving macroporous resin (MR) column chromatography and gradient countercurrent chromatography (CCC) was employed to enrich and purify bufadienolides from the roots and rhizomes of Helleborus thibetanus Franch. Initially, a D101 MR‐packed column chromatography was utilized for fractionation and enrichment of the bufadienolides, which were effectively eluted from the column using a 60% ethanol solution. CCC was subsequently introduced to separate the enriched product using the ethyl acetate/n‐butanol/water (EBuWat, 4:1:5, v/v) and EBuWat (5:0:5, v/v) solvent systems in a gradient elution mode. As results, five bufadienolides, including 6.1 mg of hellebrigenin‐3‐O‐β‐D‐glucoside (1), 2.2 mg of tigencaoside A (2), 8.3 mg of deglucohellebrin (3), 3.5 mg of 14 β‐hydroxy‐3β‐[β‐D‐glucopyranosyl‐(1→6)‐(β‐D‐glucopyranosyl)oxy]‐5α‐bufa‐20,22‐dienolide (4), and 3.0 mg of 14β‐hydroxy‐3β‐[(β‐D‐glucopyranosyl)oxy]‐5α‐bufa‐20,22‐dienolide (5), were effectively separated from 300 mg of the enriched product. The respective high‐performance liquid chromatography purities were as follows: 95.2%, 75.8%, 85.7%, 82.3%, and 92.8%. This study provides valuable insights for the efficient enrichment and separation of bufadienolides from Helleborus thibetanus Franch.

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Section: Solvent System Selection and Preparationmentioning

confidence: 99%

Separation of bufadienolides from Helleborus thibetanus Franch. by a combination approach involving macroporous resin column chromatography and gradient countercurrent chromatography

Guo,

Chen,

Zhang

et al. 2024

J of Separation Science

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“…Curcuma longa has been extensively investigated in the past for its metabolites [ 15 , 16 ]. Sesquiterpenoids and terpecurcumins extracted from C. longa L. have been studied for their anti-inflammatory, anti-atherosclerotic, and cytotoxic properties [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolome Mining of Curcuma longa L. Using HPLC-MS/MS and Molecular Networking

Budhathoki,

Timilsina,

Regmi

et al. 2023

Metabolites

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Turmeric, Curcuma longa L., is a type of medicinal plant characterized by its perennial nature and rhizomatous growth. It is a member of the Zingiberaceae family and is distributed across the world’s tropical and subtropical climates, especially in South Asia. Its rhizomes have been highly valued for food supplements, spices, flavoring agents, and yellow dye in South Asia since ancient times. It exhibits a diverse array of therapeutic qualities that encompass its ability to combat diabetes, reduce inflammation, act as an antioxidant, exhibit anticancer properties, and promote anti-aging effects. In this study, organic extracts of C. longa rhizomes were subjected to HPLC separation followed by ESI-MS and low-energy tandem mass spectrometry analyses. The Global Natural Product Social Molecular Networking (GNPS) approach was utilized for the first time in this ethnobotanically important species to conduct an in-depth analysis of its metabolomes based on their fragments. To sum it up, a total of 30 metabolites including 16 diarylheptanoids, 1 diarylpentanoid, 3 bisabolocurcumin ethers, 4 sesquiterpenoids, 4 cinnamic acid derivatives, and 2 fatty acid derivatives were identified. Among the 16 diarylheptanoids identified in this study, 5 of them are reported for the first time in this species.

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“…This method does not cause loss of chemical composition. Therefore, compared with above techniques, HSCCC has distinguished advantages in the age of green chemistry concepts [31,32]. Up to our knowledge, there is still no report on the strategy used by UTH-UAE and HSCCC to extract and separate phytochemicals from GWH.…”

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confidence: 99%

An integrated approach of homogenization‐ultrasound‐assisted extraction followed by high‐speed countercurrent chromatography for rapidly preparative separation naphthoquinones and diarylheptanes from Juglands mandshurica Maxim. exocarp

Chang,

Yu,

Yang

et al. 2023

J of Separation Science

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This research investigated the effectiveness of an integrated method for the extraction and separation of naphthoquinones and diarylheptanes from exocarp of Juglands mandshurica Maxim. (namely, green walnut husks). The target compounds were obtained by ultra‐turrax homogenization (UTH) coupled with ultrasound‐assisted extraction (UAE) technology followed by high‐speed countercurrent chromatography (HSCCC). The UTH‐UAE extraction method achieved higher efficiency with 2.49‐ and 2.36‐fold to those by UAE, and 1.39‐ and 1.34‐fold to those by UTH in a short time. HSCCC was adopted for further separation and purification; six target compounds, namely, regiolone (RE), juglone (JU), myricatomento‐genin (MG), galleon (GA), 2‐oxatrycyclo[13.2.2.13,7]eicosa‐3,5,7(20),15,17,18‐hexaen‐10‐16‐diol (OE), and juglanin A (JA), were separated with more than 95.37% purities and more than 84.71% final recovery rates, respectively. In this study, the integrated strategy of extraction and separation could get high purity compounds quickly, which would provide time and solvent saved method for the natural products separation from plants.

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Bioassay‐guided separation and identification of the anticancer composition from Curcuma longa L. by the combination strategy of methanol gradient countercurrent chromatography and ultra‐high‐performance liquid chromatography coupled with high‐resolution mass spectrometry

Cited by 5 publications

References 50 publications

Separation of bufadienolides from Helleborus thibetanus Franch. by a combination approach involving macroporous resin column chromatography and gradient countercurrent chromatography

Separation of bufadienolides from Helleborus thibetanus Franch. by a combination approach involving macroporous resin column chromatography and gradient countercurrent chromatography

Metabolome Mining of Curcuma longa L. Using HPLC-MS/MS and Molecular Networking

An integrated approach of homogenization‐ultrasound‐assisted extraction followed by high‐speed countercurrent chromatography for rapidly preparative separation naphthoquinones and diarylheptanes from Juglands mandshurica Maxim. exocarp

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