Bioassay-guided isolation of anti-inflammatory and antinociceptive glycoterpenoids from the flowers of Verbascum lasianthum Boiss. ex Bentham

Küpeli, Esra; Tatlı, I. İrem; Akdemir, Zeliha Ş.; Yeşilada, Erdem

doi:10.1016/j.jep.2006.10.004

Cited by 58 publications

(26 citation statements)

References 18 publications

(19 reference statements)

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“…Through bioassay-guided fractionation several compounds, including aucubin ( 14 ), catalpol ( 15 ), geniposidic acid ( 17 ) and ajugol ( 49 ) were isolated which have been shown in previous studies to have anti-inflammatory activity. Orally administered ucubin ( 14 ) was found to possess significant antinociceptive and anti-inflammatory activities without inducing any apparent acute toxicity or gastric damage [155]. …”

Section: Iridoid Chemistry and Researched Anti-inflammatory Iridoid-cmentioning

confidence: 99%

Anti-Inflammatory Iridoids of Botanical Origin

Viljoen

Mncwangi²,

Vermaak³

2012

CMC

174

110

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Inflammation is a manifestation of a wide range of disorders which include; arthritis, atherosclerosis, Alzheimer’s disease, inflammatory bowel syndrome, physical injury and infection amongst many others. Common treatment modalities are usually non-steroidal anti-inflammatory drugs (NSAIDs) such as aspirin, paracetamol, indomethacin and ibuprofen as well as corticosteroids such as prednisone. These however, may be associated with a host of side effects due to non-selectivity for cyclooxygenase (COX) enzymes involved in inflammation and those with selectivity may be highly priced. Thus, there is a continuing search for safe and effective anti-inflammatory molecules from natural sources. Research has confirmed that iridoids exhibit promising anti-inflammatory activity which may be beneficial in the treatment of inflammation. Iridoids are secondary metabolites present in various plants, especially in species belonging to the Apocynaceae, Lamiaceae, Loganiaceae, Rubiaceae, Scrophulariaceae and Verbenaceae families. Many of these ethnobotanicals have an illustrious history of traditional use alluding to their use to treat inflammation. Although iridoids exhibit a wide range of pharmacological activities such as cardiovascular, hepatoprotection, hypoglycaemic, antimutagenic, antispasmodic, anti-tumour, antiviral, immunomodulation and purgative effects this review will acutely focus on their anti-inflammatory properties. The paper aims to present a summary for the most prominent iridoid-containing plants for which anti-inflammatory activity has been demonstrated in vitro and / or in vivo.

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Section: Iridoid Chemistry and Researched Anti-inflammatory Iridoid-cmentioning

confidence: 99%

Anti-Inflammatory Iridoids of Botanical Origin

Viljoen

Mncwangi²,

Vermaak³

2012

CMC

174

110

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“…ex Benth. (Kupeli et al, 2007). As regards the pharmacological activity of V. phlomoides, only a few studies were carried out.…”

Section: Introductionmentioning

confidence: 99%

Correlation between polyphenol content and anti-inflammatory activity ofVerbascum phlomoides(mullein)

Grigore

Colceru-Mihul

Liţescu

et al. 2013

Pharmaceutical Biology

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Context: Verbascum phlomoides L. (Scrophulariaceae) (mullein) used in the European folk medicine due to its anti-inflammatory and soothing action on the respiratory tract is thoroughly documented in handbooks and scientific literature. Nevertheless, information regarding the influence of the polyphenol content on pharmacological activity is scarce. Objective: This study explored the antioxidant and anti-inflammatory potential of V. phlomoides polyphenol-rich extract. Materials and methods: Dried mullein flowers (200 g) were subjected to water extraction (60 C, 2 h, herb/solvent ratio ¼ 1/10 m/v) and further to n-butanol partition. Total phenolics were spectrophotometrically determined and specific compounds were evaluated by HPLC. The antioxidant activity was assessed by the 2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH) assay. The anti-inflammatory potential of the extract (50-200 mg/mL) was evaluated in vitro by ELISA measurement of ICAM-1 expression in TNF-a-stimulated endothelial cells and in vivo by the rat paw edema assay. Results: The mullein extract contained 4.18% total polyphenols expressed as gallic acid. The main components identified by HPLC were: rosmarinic acid (14.93 mg/g), caffeic acid (39.96 mg/g), ferulic acid (29.61 mg/g) and quercetin (17.29 mg/g). Acteoside was not detected; aucubin was detected in traces (0.028 mg/g). Depending on concentration, the extract exerted scavenging activity on DPPH radical (EC 50 7.09 mg/mL), significantly inhibited TNF-a-induced ICAM-1 expression by 55-58.8% on human umbilical vein endothelial cells at 100 and 200 mg/mL, but failed to reduce egg-white-induced rat paw edema. Discussion and conclusion: Mullein polyphenols play an important role in exerting the antioxidant effect but have a weak influence on anti-inflammatory activity that is correlated, probably, to a higher content of iridoids and phenylethanoids.

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“…A methanolic extract of the flowers was shown to possess significant inhibitory activity in the carrageenan-induced hind paw edema model and in p-benzoquinoneinduced writhings in mice. Through bioassayguided fractionation and isolation procedures, a triterpenoid saponin, ilwensisaponin A, was found to be the antinociceptive and anti-inflammatory principles, without inducing any apparent acute toxicity or gastric damage, while the iridoid glucoside ajugol had no activity (Küpeli et al, 2007). Ilwensisaponin A (1) and ajugol (3) were also isolated from the flowers of V. pterocalycinum var.…”

Section: Resultsmentioning

confidence: 99%

Antinociceptive and Anti-Inflammatory Effects of Saponin and Iridoid Glycosides from Verbascum pterocalycinum var. mutense Hub.-Mor.

Akkol

Tatlı

Akdemir

2007

Zeitschrift Für Naturforschung C

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The anti-inflammatory and antinociceptive properties of four major compounds from the flowers of Verbascum pterocalycinum var. mutense were investigated. Saponin glycosides called ilwensisaponin A and C and iridoid glycosides known as ajugol and picroside IV were isolated from the methanolic extract. A dose-related anti-inflammatory and antinociceptive response were obtained in this study at doses of 100 and 200 mg/kg. The results of the evaluation of the anti-inflammatory activity induced by carrageenan and PGE 1 showed that this species possesses active constituents that could diminish the cyclooxygenase activitiy. No effects were observed in the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema model. Our results support the anti-inflammatory and analgesic effects of Verbascum pterocalycinum var. mutense. Ilwensisaponins A and C could explain in part the anti-inflammatory and analgesic activities of this species. Although antinociceptive and anti-inflammatory activities of ajugol and picroside IV were found insignificant in the statistical analysis, ilwensisaponin A and C showed notable activity without inducing any apparent acute toxicity as well as gastric damage.

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Bioassay-guided isolation of anti-inflammatory and antinociceptive glycoterpenoids from the flowers of Verbascum lasianthum Boiss. ex Bentham

Cited by 58 publications

References 18 publications

Anti-Inflammatory Iridoids of Botanical Origin

Anti-Inflammatory Iridoids of Botanical Origin

Correlation between polyphenol content and anti-inflammatory activity ofVerbascum phlomoides(mullein)

Antinociceptive and Anti-Inflammatory Effects of Saponin and Iridoid Glycosides from Verbascum pterocalycinum var. mutense Hub.-Mor.

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