Bioassay-Guided Isolation and Characterization of Metabolites from Actinobacteria

Balagurunathan, Ramasamy; Radhakrishnan, Manikkam; Shanmugasundaram, Thangavel; Venugopal, Gopikrishnan; Joseph, Jerrine

doi:10.1007/978-1-0716-0728-2_8

Cited by 3 publications

(2 citation statements)

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“…Conventional methods require an organic solvent or water, while modern techniques depend on an elevated temperature and pressure [ 94 ]. The Liquid–liquid extraction method or partitioning is commonly utilized for the extraction of secondary metabolites from actinobacteria [ 95 ]. Furthermore, the selection of the solvent is essential since extraction is based on the law of similarities and intermiscibility (polarities); both the solvent and solute should have near equivalent polarity values for efficient extraction [ 93 ].…”

Section: Fermentation and Extraction Of Bioactive Compounds From Acti...mentioning

confidence: 99%

“…The compounds present in the crude extract are rather complex with physical and chemical differences, thus requiring further separation and purification. Chromatography (thin-layer chromatography, column chromatography, and high-performance liquid chromatography) is the frequently used technique to acquire pure natural compounds [ 95 ].…”

Section: Fermentation and Extraction Of Bioactive Compounds From Acti...mentioning

confidence: 99%

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Marine Actinomycetes Associated with Stony Corals: A Potential Hotspot for Specialized Metabolites

et al. 2022

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Microbial secondary metabolites are an important source of antibiotics currently available for combating drug-resistant pathogens. These important secondary metabolites are produced by various microorganisms, including Actinobacteria. Actinobacteria have a colossal genome with a wide array of genes that code for several bioactive metabolites and enzymes. Numerous studies have reported the isolation and screening of millions of strains of actinomycetes from various habitats for specialized metabolites worldwide. Looking at the extent of the importance of actinomycetes in various fields, corals are highlighted as a potential hotspot for untapped secondary metabolites and new bioactive metabolites. Unfortunately, knowledge about the diversity, distribution and biochemistry of marine actinomycetes compared to hard corals is limited. In this review, we aim to summarize the recent knowledge on the isolation, diversity, distribution and discovery of natural compounds from marine actinomycetes associated with hard corals. A total of 11 new species of actinomycetes, representing nine different families of actinomycetes, were recovered from hard corals during the period from 2007 to 2022. In addition, this study examined a total of 13 new compounds produced by five genera of actinomycetes reported from 2017 to 2022 with antibacterial, antifungal and cytotoxic activities. Coral-derived actinomycetes have different mechanisms of action against their competitors.

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Section: Fermentation and Extraction Of Bioactive Compounds From Acti...mentioning

confidence: 99%

Section: Fermentation and Extraction Of Bioactive Compounds From Acti...mentioning

confidence: 99%

Marine Actinomycetes Associated with Stony Corals: A Potential Hotspot for Specialized Metabolites

et al. 2022

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Biological potential of bioactive metabolites derived from fungal endophytes associated with medicinal plants

et al. 2021

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Elicitation of Cryptic Secondary Metabolites and Antibacterial Activities from Mangrove and Cave Soil Actinomycetes

Ahmad Razi,

Ab Ghani,

Sadiran

et al. 2024

JST

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Actinomycetes’ secondary metabolites have received considerable attention due to their many beneficial biological activities. However, many biosynthetic gene clusters in actinomycetes remain silent as they are not transcribed under standard laboratory conditions. Therefore, this study aims to introduce antibiotic elicitors to activate cryptic secondary metabolites in soil actinomycetes and screen them for antibacterial potential. A total of 20 cave and 10 mangrove soil actinomycete isolates were exposed to streptomycin or erythromycin at subinhibitory concentration (0.5–1048 μg/mL) in minimal media. The ethyl acetate extracts were subjected to high-performance liquid chromatography (HPLC) profiling to observe the effect of elicitors towards secondary metabolite production. As a result, 61.7% of the isolates showed a positive impact (appearance of ‘new’/increase in metabolite production) when elicitors were supplemented. These changes were more pronounced in erythromycin-induced media (63.3%) than in streptomycin (60.0%). Two isolates (CS3PT50 and CS3PT53) exhibited significant changes in the profile, with additional peaks detected at 210 and 245 nm, which may indicate the production of new metabolites. More antibacterial activities were observed from stimulated (26.7%) as opposed to non-stimulated isolates (10.0%), including 6 new activities, 1 improved, and 1 decrease in inhibitory. Furthermore, isolate CS3PT53 (0.5 mg/disc) displayed broad-spectrum activities, inhibiting S. aureus ATCC 25923 and S. Typhimurium ATCC 14028. The hit actinomycete isolates belonged to the genus Streptomyces (55.6%), Norcardia (22.2%), Norcardiopsis, and Saccharomonospora (11.1%). Overall, this study demonstrated that incorporating antibiotic elicitor at subinhibitory concentration could effectively trigger the production of cryptic secondary metabolites with antibacterial properties in soil actinomycetes.

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Bioassay-Guided Isolation and Characterization of Metabolites from Actinobacteria

Cited by 3 publications

References 10 publications

Marine Actinomycetes Associated with Stony Corals: A Potential Hotspot for Specialized Metabolites

Marine Actinomycetes Associated with Stony Corals: A Potential Hotspot for Specialized Metabolites

Biological potential of bioactive metabolites derived from fungal endophytes associated with medicinal plants

Elicitation of Cryptic Secondary Metabolites and Antibacterial Activities from Mangrove and Cave Soil Actinomycetes

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