Bioartificial Organs: Ongoing Research and Future Trends

Bartolo, Loredana De; Diego, Mantovani

doi:10.1159/000518251

Cited by 2 publications

(2 citation statements)

References 9 publications

(7 reference statements)

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“…The ideal biomaterial to form a reliable hydrogel must be biocompatible, biodegradable, and non-toxic to promote cell adhesion, growth, and differentiation [9], [10]. Alginate is a natural polysaccharide approved by the FDA and widely used as a "smart" biomaterial and as a bio-ink when loaded with cells [11], [12].…”

Section: Introductionmentioning

confidence: 99%

Tailoring Alginate-Gelatin Hydrogels to Precisely Modulate Osteogenesis in Dental Pulp Stem Cells While Preserving Other Cellular Behaviors

Ferjaoui,

López-Muñoz,

Akbari

et al. 2024

Preprint

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Alginate-gelatin (Alg-Gel) hydrogels have been used experimentally but not clinically associated with mesenchymal stromal / stem cells (MSCs) to guide bone tissue formation. One of the main challenges for its clinical application is optimizing Alg-Gel stiffness to guide osteogenesis. In this study, we investigated how Alg-Gel stiffness could modulate the dental pulp stem cell (DPSCs) attachment, morphology, proliferation, and osteogenic differentiation, identifying the optimal condition to uncouple osteogenesis from the other cell behaviors. An array of Alg-Gel hydrogels was prepared by casting different percentages of Alg and Gel being crosslinked with 2 % CaCl2. We selected two hydrogels, one with 11± 1 kPa called “low” stiffness and one with 55 ± 3 kPa called “high” stiffness. Hydrogel analyses showed that the average swelling rates were 20 ± 3% for low and 35 ± 2% for high hydrogels. The degradation percentage was 47 ± 5% and 18 ± 2% for low and high hydrogels, respectively. Both hydrogel types showed homogeneous surface shape and pro-tein (Alg-Gel) interaction with CaCl2 as assessed by FTIR-ATR and XPS. Cell culture showed good adhesion of the DPSCs to the hydrogels and proliferation. Furthermore, better osteogenic activity was obtained with high-stiffness hydrogels. In summary, this study confirms the possibility of characterizing and optimizing the stiffness of alginate-gelatin gel to guide osteogenesis in vitro without altering other cellular properties of DPSCs.

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Section: Introductionmentioning

confidence: 99%

Tailoring Alginate-Gelatin Hydrogels to Precisely Modulate Osteogenesis in Dental Pulp Stem Cells While Preserving Other Cellular Behaviors

Ferjaoui,

López-Muñoz,

Akbari

et al. 2024

Preprint

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“…Over the past years, natural polymers such as collagen, fibrinogen, and elastin were successfully electrospun to nanofibers for potential uses such as tissue engineering scaffolds, Nanomaterials 2023, 13, 1359 2 of 18 wound dressings, and various other biomedical applications [27][28][29][30][31]. A growing interest exists in creating new materials by blending natural polymers with synthetic ones [32,33]. Blending polymers can produce new materials with distinctive structural, mechanical and biochemical properties.…”

Section: Introductionmentioning

confidence: 99%

The Mechanical Properties of Blended Fibrinogen:Polycaprolactone (PCL) Nanofibers

2023

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Electrospinning is a process to produce versatile nanoscale fibers. In this process, synthetic and natural polymers can be combined to produce novel, blended materials with a range of physical, chemical, and biological properties. We electrospun biocompatible, blended fibrinogen:polycaprolactone (PCL) nanofibers with diameters ranging from 40 nm to 600 nm, at 25:75 and 75:25 blend ratios and determined their mechanical properties using a combined atomic force/optical microscopy technique. Fiber extensibility (breaking strain), elastic limit, and stress relaxation times depended on blend ratios but not fiber diameter. As the fibrinogen:PCL ratio increased from 25:75 to 75:25, extensibility decreased from 120% to 63% and elastic limit decreased from a range between 18% and 40% to a range between 12% and 27%. Stiffness-related properties, including the Young’s modulus, rupture stress, and the total and relaxed, elastic moduli (Kelvin model), strongly depended on fiber diameter. For diameters less than 150 nm, these stiffness-related quantities varied approximately as D−2; above 300 nm the diameter dependence leveled off. 50 nm fibers were five–ten times stiffer than 300 nm fibers. These findings indicate that fiber diameter, in addition to fiber material, critically affects nanofiber properties. Drawing on previously published data, a summary of the mechanical properties for fibrinogen:PCL nanofibers with ratios of 100:0, 75:25, 50:50, 25:75 and 0:100 is provided.

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Bioartificial Organs: Ongoing Research and Future Trends

Cited by 2 publications

References 9 publications

Tailoring Alginate-Gelatin Hydrogels to Precisely Modulate Osteogenesis in Dental Pulp Stem Cells While Preserving Other Cellular Behaviors

Tailoring Alginate-Gelatin Hydrogels to Precisely Modulate Osteogenesis in Dental Pulp Stem Cells While Preserving Other Cellular Behaviors

The Mechanical Properties of Blended Fibrinogen:Polycaprolactone (PCL) Nanofibers

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