Bioartificial Biomaterials for Regenerative Medicine Applications

Chiono, Valeria; Nardò, Tiziana; Ciardelli, Gianluca

doi:10.1016/b978-0-12-398523-1.00009-4

Cited by 4 publications

(2 citation statements)

References 188 publications

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“…For instance, an elastomeric PU scaffold has been recently developed by Chiono et al as a substrate for CPC culture [ 88 ]. Synthetic biomaterials can be functionalized with ECM proteins or integrin-binding peptides as well as growth factors to improve cell attachment, proliferation and differentiation, obtaining “bioartificial” materials [ 89 , 90 ]. Indeed, Boffito et al have shown that the surface functionalization of elastomeric PU scaffolds with the cardiac ECM protein laminin-1 enhances CPC proliferation as well as early differentiation into CMs, smooth muscle cells and endothelial cells and protects CPCs from apoptosis [ 91 ].…”

Section: Biomaterials For Guiding Cell Behaviourmentioning

confidence: 99%

Impact of Biomaterials on Differentiation and Reprogramming Approaches for the Generation of Functional Cardiomyocytes

Paoletti

Divieto

Chiono

2018

Cells

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The irreversible loss of functional cardiomyocytes (CMs) after myocardial infarction (MI) represents one major barrier to heart regeneration and functional recovery. The combination of different cell sources and different biomaterials have been investigated to generate CMs by differentiation or reprogramming approaches although at low efficiency. This critical review article discusses the role of biomaterial platforms integrating biochemical instructive cues as a tool for the effective generation of functional CMs. The report firstly introduces MI and the main cardiac regenerative medicine strategies under investigation. Then, it describes the main stem cell populations and indirect and direct reprogramming approaches for cardiac regenerative medicine. A third section discusses the main techniques for the characterization of stem cell differentiation and fibroblast reprogramming into CMs. Another section describes the main biomaterials investigated for stem cell differentiation and fibroblast reprogramming into CMs. Finally, a critical analysis of the scientific literature is presented for an efficient generation of functional CMs. The authors underline the need for biomimetic, reproducible and scalable biomaterial platforms and their integration with external physical stimuli in controlled culture microenvironments for the generation of functional CMs.

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Section: Biomaterials For Guiding Cell Behaviourmentioning

confidence: 99%

Impact of Biomaterials on Differentiation and Reprogramming Approaches for the Generation of Functional Cardiomyocytes

Paoletti

Divieto

Chiono

2018

Cells

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“…The main limitations of the previous studies are the use of mouse or rat cardiac cells, which are not predictive of human cell response, and weak ability of hydrogels in providing structural cues. To address some of these limitations, “bioartificial” scaffolds, based on synthetic and natural polymers could provide biomimetic substrates with tailor-designed architecture, able to combine the cell recognition properties of proteins with the processability, shape stability, slow degradation rate and superior mechanical resistance of hydrogels ( Ciardelli et al, 2005 ; Chiono et al, 2009 ; Chiono et al, 2014 ). For example, Kai et al (2011) obtained rabbit CMs alignment on oriented electrospun PCL/gelatin nanofibrous scaffolds, mimicking ECM orientation of healthy myocardium.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic design of bioartificial scaffolds for the in vitro modelling of human cardiac fibrosis

Spedicati

Ruocco

Zoso

et al. 2022

Front. Bioeng. Biotechnol.

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In vitro models of pathological cardiac tissue have attracted interest as predictive platforms for preclinical validation of therapies. However, models reproducing specific pathological features, such as cardiac fibrosis size (i.e., thickness and width) and stage of development are missing. This research was aimed at engineering 2D and 3D models of early-stage post-infarct fibrotic tissue (i.e., characterized by non-aligned tissue organization) on bioartificial scaffolds with biomimetic composition, design, and surface stiffness. 2D scaffolds with random nanofibrous structure and 3D scaffolds with 150 µm square-meshed architecture were fabricated from polycaprolactone, surface-grafted with gelatin by mussel-inspired approach and coated with cardiac extracellular matrix (ECM) by 3 weeks culture of human cardiac fibroblasts. Scaffold physicochemical properties were thoroughly investigated. AFM analysis of scaffolds in wet state, before cell culture, confirmed their close surface stiffness to human cardiac fibrotic tissue. Following 3 weeks culture, biomimetic biophysical and biochemical scaffold properties triggered the activation of myofibroblast phenotype. Upon decellularization, immunostaining, SEM and two-photon excitation fluorescence microscopy showed homogeneous decoration of both 2D and 3D scaffolds with cardiac ECM. The versatility of the approach was demonstrated by culturing ventricular or atrial cardiac fibroblasts on scaffolds, thus suggesting the possibility to use the same scaffold platforms to model both ventricular and atrial cardiac fibrosis. In the future, herein developed in vitro models of cardiac fibrotic tissue, reproducing specific pathological features, will be exploited for a fine preclinical tuning of therapies.

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Biodegradable Polymer for Membranes

Salerno¹

2014

Encyclopedia of Membranes

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Bioartificial Biomaterials for Regenerative Medicine Applications

Cited by 4 publications

References 188 publications

Impact of Biomaterials on Differentiation and Reprogramming Approaches for the Generation of Functional Cardiomyocytes

Impact of Biomaterials on Differentiation and Reprogramming Approaches for the Generation of Functional Cardiomyocytes

Biomimetic design of bioartificial scaffolds for the in vitro modelling of human cardiac fibrosis

Biodegradable Polymer for Membranes

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