Bioanalytical RP‐HPLC method for the determination of ponazuril in plasma

Abstract

The goal of this investigation was to establish a reliable technique for the quantitation of ponazuril in limited sample volumes. Samples were extracted with chloroform and separation was achieved with a Symmetry RP 18 column. Ultraviolet absorption was measured at 254 nm. A combination of 0.1% formic acid and acetonitrile (50:50) was used as the mobile phase. The calibration curve was linear from 0.1-25 μg/mL, with a lower limit of quantification of 0.1 μg/mL with a 100 μL sample. The precision and accuracy were well within the range set by the Food and Drug Administration and the recovery was over 95%. This technique was used to analyze ponazuril samples and found to be appropriate for pharmacokinetic studies.

Pharmacokinetics of orally administered single‐dose ponazuril in cats

“…Ponazuril was extracted from plasma samples using a liquid‐liquid extraction method. (Cox et al., 2021) Previously frozen plasma samples were thawed, vortex‐mixed, and 100 µl of plasma was transferred to a 13 × 100 mm screw top tube followed by 10 µl of diclazuril (internal standard, 100 µg/ml) and 2 ml chloroform. The tubes were rocked for 15 min and then centrifuged for 20 min at 1000 g .…”

Application of peptide biomarkers in life analysis based on liquid chromatography–mass spectrometry technology

“…HPLC is a popular technique for the separation, identification, and purification of biologically active peptides 33,34 . At present, the commonly used methods of HPLC for peptide separation and analysis include reversed‐phase high‐performance liquid chromatography (RP‐HPLC), 35 ion exchange chromatography (IEC), 36 size exclusion chromatography (SEC), 37 affinity chromatography (AC), 38 multidimensional high‐performance liquid chromatography (MD‐HPLC), 39 and so forth.…”