Bioanalytical method for the simultaneous quantification of irinotecan and its active metabolite SN-38 in mouse plasma and tissue homogenates using HPLC-fluorescence

Carboxylesterase 1 family knockout alters drug disposition and lipid metabolism

“…Levels of irinotecan and SN-38 were analyzed by HPLC-fluorescence as described previously. The lower limit of quantification for irinotecan was 7.5 ng/mL, and for SN-38 5 ng/mL 23 .…”

“…to male WT and Ces1 –/– mice. Levels of irinotecan and SN-38 in plasma were analyzed by HPLC-fluorescence 23 . Plasma AUCs of irinotecan were 17.2- and 1.9-fold higher in Ces1 –/– versus WT mice after oral and i.v.…”

Fe‐containing metal–organic framework with D‐penicillamine for cancer‐specific hydrogen peroxide generation and enhanced chemodynamic therapy

“…A mobile phase composed of acetonitrile and 0.1% formic acid (v/v = 60:40) was pumped at a rate of 0.7 ml/min. A peak was detected at a wavelength of 360 nm 34 . To measure the loading amount of calcein, the collected supernatant containing calcein was assayed spectrophotometrically at 495 nm using a UV–Vis spectrophotometer (UV‐1800; Shimadzu) 35 …”

Lipid-assisted PEG-b-PLA nanoparticles with ultrahigh SN38 loading capability for efficient cancer therapy

“…The morphology of the above nanoparticles was examined via transmission electron microscopy (TEM) (Thermo Fisher Talos L120C). The loading efficiency (LE) and loading capacity (LC) of SN38 was determined via high performance liquid chromatography (HPLC) 21–23 using a 2695 HPLC system (Waters, USA) and calculated based on the following equations through triplicate experiments.…”

“…The morphology of the above nanoparticles was examined via transmission electron microscopy (TEM) (Thermo Fisher Talos L120C). The loading efficiency (LE) and loading capacity (LC) of SN38 was determined via high performance liquid chromatography (HPLC) [21][22][23] Crystallization of SN38 was monitored via scanning electron microscopy (SEM) and X-ray diffraction (XRD). 24 For SEM analysis, organic solutions of PEG-b-PLA and SN38 with or without lipids at a weight ratio of SN38 : PEG-b-PLA : lipid of 1 : 5 : 0.5 or 1 : 5 : 0 were prepared and then 2-3 drops of the solutions…”