2010
DOI: 10.1002/bmc.1433
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Bioanalytical method development and validation of novel antithrombotic agent S002‐333 by LC‐MS/MS and its application to pharmacokinetic studies

Abstract: A rapid, sensitive and simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method using an electrospray ionization (ECI) source for the quantification of novel anti-thrombotic agent S002-333 [2-(4-methoxy-benzenesulfonyl)-2,3,4,9-tetrahydro-1H-β-carboxylic acid amide] in rabbit plasma was developed and validated. The extraction from plasma was carried out by simple protein precipitation extraction method. The chromatographic separation was performed on an Ultramex Cyno, (150 x 4.6 mm, 5 µm) with a… Show more

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Cited by 8 publications
(7 citation statements)
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References 9 publications
(9 reference statements)
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“…The MS/MS parameters were optimized using the Analyst software ‘Quantitative Optimization wizard’ to achieve maximum responses of dexamethasone and IS with reasonable values of parameters. The optimized precursor‐to‐product ion transitions monitored for DEX were m / z 393.0/147.1 while testosterone (IS) was monitored at m / z 289.5/97.3, which corresponded to the previous reports (Bhatta et al ., ; Yang et al ., ).…”
Section: Resultsmentioning
confidence: 99%
“…The MS/MS parameters were optimized using the Analyst software ‘Quantitative Optimization wizard’ to achieve maximum responses of dexamethasone and IS with reasonable values of parameters. The optimized precursor‐to‐product ion transitions monitored for DEX were m / z 393.0/147.1 while testosterone (IS) was monitored at m / z 289.5/97.3, which corresponded to the previous reports (Bhatta et al ., ; Yang et al ., ).…”
Section: Resultsmentioning
confidence: 99%
“…3,4 The use of antiplatelet agents; aspirin, ticlopidine, and clopidogrel do not work in acute conditions and require extended periods of strictly supervised therapy mainly because of the high risk of bleeding. [7][8][9][10][11] The S002-333 is practically insoluble in water and hexane, where as it is most soluble in acetone, acetonitrile and chloroform (>10 mg mL À1 ). 1).…”
Section: Introductionmentioning
confidence: 99%
“…11 The antithrombotic agent, S002-333 suffers from poor water solubility, thus presenting major hurdles in the dissolution leading to poor bioavailability. A collagen mediated platelet activation (aggregation and adhesion) and release reaction is reduced by S002-333, which results in reduction in human platelet aggregation.…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…This method was applied to determine the concentration of K‐09 and metabolite K‐9T in plasma after oral administration to a male hamster at a dose of 25 mg/kg. LC‐MS/MS is currently considered the method of choice for supporting preclinical and clinical pharmacokinetic studies (Bhatta et al , ). In the drug discovery and development process, identification of a new compound with potent biological activity is an important step, whereas ADME (absorption, distribution, metabolism and excretion) is a critical factor in determining the success of a new active compound as a suitable therapeutic agent.…”
Section: Introductionmentioning
confidence: 99%