Bioanalytical Method Development and Validation for the Determination of Levocetirizine in Pharmaceutical Dosage Form and Human Plasma by RP-HPLC

Abstract

This study describes the development of a rapid, selective, precise and sensitive reverse phase high -performance liquid chromatography method for the quantitative determination of Levocetirizine Dihydrochloride (LCD) in human plasma and pharmaceutical dosage form. Extraction of drug from plasma was done by employing optimized liquid-liquid extraction procedure. The sample was analyzed using Acetonitrile: Methanol: 20mM Ammonium Acetate Buffer pH-5 (25:55:20 % v/v/v) as mobile phase. Chromatographic separation was achieved on Prontosil C-18 column (4.6 x 250mm, 5μ particle size) as stationary phase using isocratic elution (at a flow rate of 1 mL/min). The peak was detected using UV-PDA detector set at 232 nm and retention time was found to be 8 min for LCD. The calibration curve obtained was linear (r 2 = 0.9998) over the concentration range of 2-10 μg/mL. Method was validated for precision, robustness and recovery. The limit of detection (LOD) and limit of quantitation (LOQ) was 0.0057 and 0.174 µg/mL respectively. There was no significant difference between the amount of drug spiked in plasma and the amount recovered and plasma did not interfere in estimation. Thus, the proposed method is suitable for the analysis of LCD in tablet dosage forms and human plasma.

Synthesis of E -stilbene azomethines as potent antimicrobial and antioxidant agents

“…1 One example of such compounds is resveratrol (3,4,5-trihydroxystilbene), which was first isolated from the Japanese plant Veratrum grandiflorum. 2 This compound has demonstrated significant antineoplastic activity, 3 angiogenesis, 4 action against certain microbes 5 and inflammation, 6 activity against acquired immunodeficiency syndrome, 7 radical scavenging, 8 and platelet aggregation. 9 Other reported biological activities of resveratrol include antagonist action against aryl hydrocarbons receptors (AhR) 10 , enhancement of estrogenic potency, 10 neuroprotective effects, 8 cAMP phosphodiesterase inhibition, 11 and slowed progression of atherosclerosis.…”

Analytical Method by HPLC-Dad Allows Quantification of Quercetin Marker in Standardized Extract of Anadenanthera Colubrina Var. Cebil

“…The development of analytical methods must be based on fundamental conditions such as sampling, pretreatment sampling, and the evaluation of validation parameters in order to guarantee the quality, consistency, and reliability of the analytical results [17][18]. The pretreatment of samples is a decisive step in the development of the analytical methods of constituents in biological matrices and must guarantee the release of the analytes of the complex matrix, to be monitored by analytical signals with appropriate instrumentation [19][20][21].…”

Development and Validation of High-Performance Liquid Chromatography With Diode Array Detector Method to Analysis of Kaempferol Marker From Extracts of Poincianella Pyramidalis (Tul) L.P Queiroz

“…Considering all proven pharmacological activities and their large number of secondary metabolites, this vegetable species represents potential candidate for an herbal medicine product; however, some challenges for the drug development and quality control should be addressed [13,14]. One of the most important requirements is an analytical methodology for quality control, since the raw material to finished product and in the case of natural products, appropriate standardization of vegetal drug [13][14].…”

“…One of the most important requirements is an analytical methodology for quality control, since the raw material to finished product and in the case of natural products, appropriate standardization of vegetal drug [13][14].…”