Bioactivity studies on Musa seminifera Lour

BackgroundConsumption of vegetables has been proven to be effective in the prevention of different diseases. Traditionally edible aerial part of Pisum sativum L. subsp. sativum (Fabaceae) is used to treat diabetes, heart diseases and as blood purifier. Present study was aimed to explore the traditional use of aerial parts of P. sativum as a source of antidiabetic agent. In addition, antioxidant activity and chemical composition was carried out.MethodsTotal polyphenol content was spectrophotometrically determined using Folin Chiocalteu’s reagent while the flavonoids by aluminum chloride colorimetric assay. Identification of compounds of the extract was made through HPLC and LCMS. Antihyperglycemic activity was assessed by oral glucose tolerance test in mice. Antioxidant activity was determined by DPPH free radical scavenging and reducing power assay.ResultsTotal polyphenol and total flavonoids content were found to be 51.23 mg gallic acid equivalent and 30.88 mg quercetin equivalent per gram of dried plant extract respectively. Ellagic acid and p-coumeric acid were detected through HPLC. A total of eight compounds including naringenin, β-sitosterol were indentified through LCMS. In OGTT, extract (200 mg/kg bw) showed a 30.24% decrease (P< 0.05) in blood glucose levels at 30 min compared to the normal control. The extract showed IC50 value of 158.52 μg/mL in DPPH scavenging assay and also showed comparable reducing power.ConclusionAlong with other compounds ellagic acid and β-sitosterol present in the extract may be responsible for its antioxidant as well as antihyperglycemic activities. Altogether these results rationalize the use of this vegetable in traditional medicine.

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“…After 10mins, absorbance of the mixture was measured at 700 nm. Butylated Hydroxy Toluene (BHT) was used as standard [20]. …”

Section: Methodsmentioning

confidence: 99%

Chemical composition and pharmacological activities of Pisum sativum

Zilani

Sultana

Rahman

et al. 2017

BMC Complement Altern Med

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“…The reducing power was determined according to the method of Sanjib et al [16]. An alequite of 1 ml of various concentrations of extracts (10-100 μg/ml) were mixed with 2.5 ml of 200 mmol/l sodium phosphate buffer (pH 6.6) and 2.5 ml of 1% potassium ferricyanide.…”

Section: Reducing Power Assaymentioning

confidence: 99%

Bioactivities of Bruguiera gymnorrhiza and profiling of its bioactive polyphenols by HPLC-DAD

et al. 2017

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Background: In folk medicine leaves and stem of Bruguiera gymnorrhiza (L.) are commonly used to treat diarrhea, fever, diabetes, pain and a number of ailments. The present study was carried out to explore antioxidant, analgesic and antidiarrhoeal activities of ethanol extract of leaves and stem of B. gymnorrhiza and also to analyze its major bioactive natural polyphenols by HPLC-DAD. Methods: Total polyphenol content was spectrophotometrically determined using Folin Chiocalteu's reagent while the flavonoids by aluminum chloride colorimetric assay. Antioxidant activity was determined by DPPH free radical scavenging, reducing power, nitric oxide and hydrogen peroxide scavenging assays. Identification and quantification of bioactive polyphenols were done by HPLC-DAD method. Antidiarrhoeal activity of the extracts was evaluated using experimentally castor oil induced diarrhea in mice. Acetic acid induced writhing method was used to evaluate the analgesic activity. Acute oral toxicity and brine shrimp lethality assay were performed to check the cytotoxic potential. Results: Both the leave and stem extracts contain significant amount of phenolic and flvonoid content. Extracts showed DPPH radical scavenging, nitric oxide, hydrogen peroxide scavenging and also concentration dependent reducing power activity. HPLC analysis of both extract indicated the presence of significant amount of vanillic acid along with other phenolic constituents. Both extracts showed significant (P < 0.01) analgesic and antidiarrhoeal activity. Furthermore, extracts showed negligible toxic effect. Conclusion: Along with other phenolic compounds, vanillic acid present in the extract may be responsible for antioxidant, analgesic and antidiarrhoeal activities. Altogether these results rationalize the use of this plant in traditional medicine.

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“…antimicrobial, 7 and antifungal 8 properties, while the flower extract was reported to have DPPH free radical scavenging and cytotoxic activity. 9 As a part of the continuation of our research on bioactivity screening of Bangladeshi medicinal plants, 10,11,12 here we report the antiinflammatory, antioxidant activity and quantification of the major polyphenols of ethanol and water extracts of A. hispida leaves.…”

Section: Introductionmentioning

confidence: 99%