Bioactivity studies and adhesion of human osteoblast (hFOB) on silicon-biphasic calcium phosphate material

Ibrahim, Suhaina Mohd; Sabudin, Salina; Sahid, Sudirman; Marzuke, Muhamad Anas; Hussin, Zul Hazmi; Bashah, Nor Shahida Kader; Jamuna-Thevi, K.

doi:10.1016/j.sjbs.2015.10.024

Cited by 24 publications

(14 citation statements)

References 21 publications

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“…PPY/PLCL fibers were biodegradable over time and the complete conduit structure was undetectable at 8 weeks post-implantation. Importantly, the rough surface of PPY/PLCL scaffolds due to PPY particles coated over PLCL film makes it proper for cell adhesion and proliferation (Ibrahim et al, 2016). Conductive materials along with ES can promote PC12 cell proliferation, migration and neurite extension (Kang et al, 2011; Pires et al, 2015; Su and Shih, 2015).…”

Section: Discussionmentioning

confidence: 99%

Polymerizing Pyrrole Coated Poly (l-lactic acid-co-ε-caprolactone) (PLCL) Conductive Nanofibrous Conduit Combined with Electric Stimulation for Long-Range Peripheral Nerve Regeneration

Song

Sun

Liu

et al. 2016

Front. Mol. Neurosci.

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Electrospinning and electric stimulation (ES) are both promising methods to support neuron adhesion and guide extension of neurons for nerve regeneration. Concurrently, all studies focus on either electrospinning for conduits material or ES in vitro study to accelerate nerve regeneration; few work on the combined use of these two strategies or ES in vivo study. Therefore, this study aimed to investigate the abilities of direct current ES through electrospinning conductive polymer composites composed of polypyrrole and Poly (l-lactic acid-co-ε-caprolactone) (PPY/PLCL) in peripheral nerve regeneration. PPY/PLCL composite conduits were synthesized by polymerizing pyrrole coated electrospun PLCL scaffolds. Morphologies and chemical compositions were characterized by scanning electron microscope and attenuated total reflection fourier transform infrared (ATR-FTIR) microscope. Rat pheochromocytoma 12 (PC12) cells and dorsal root ganglia (DRG) cells cultured on PPY/PLCL scaffolds were stimulated with 100 mV/cm for 4 h per day. The median neurite length and cell viability were measured in PC-12 cells. The levels of brain-derived neurotrophic factor (BDNF), glial cell derived neurotrophic factor (GDNF) and neurotrophin-3 (NT-3) were analyzed in DRG cells. In rats, 15 mm gaps of sciatic nerves were bridged using an autograft, non-stimulated PPY/PLCL conduit and PPY/PLCL conduit stimulated with 100 mV potential, respectively. A 100 mV potential direct current ES was applied for 1 h per day at 1, 3, 5 and 7 days post-implantation. The PPY/PLCL conduits with ES showed a similar performance compared with the autograft group, and significantly better than the non-stimulated PPY/PLCL conduit group. These promising results show that the PPY/PLCL conductive conduits’ combined use with ES has great potential for peripheral nerve regeneration.

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Section: Discussionmentioning

confidence: 99%

Polymerizing Pyrrole Coated Poly (l-lactic acid-co-ε-caprolactone) (PLCL) Conductive Nanofibrous Conduit Combined with Electric Stimulation for Long-Range Peripheral Nerve Regeneration

Song

Sun

Liu

et al. 2016

Front. Mol. Neurosci.

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“…These electrospun biphasic (HA/β-TCP) nanobioceramic scaffolds were utilised in this study to monitor hFOB 1.19 osteoblast cells' response to these scaffolds. We particularly focussed on the mineralisation of the osteoblast cells after a number of days growing on the electrospun scaffolds [31,32], as well as the production of Nitric Oxide (NO), since it is a signal of possible early increased formation of bone [33]. Our group also investigated the expression of endothelial nitric oxide (eNOS) and inducible nitric oxide (iNOS) with specific antibodies and visualised it with confocal microscopy [34].…”

Section: Introductionmentioning

confidence: 99%

hFOB 1.19 osteoblast cells grown on a biomimetic biphasic nanoscaffold: An in vitro evaluation for possible bone tissue engineering

Preez¹,

Richter²,

Papendorp³

et al. 2018

biomedicalresearch

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Much research over the past five decades has focussed on the repair and replacement of bone. Recently, the research focus has shifted to nanotechnology since it provides a platform from which to alter and possibly improve materials' properties. In this study we have made use of previously developed electrospun biphasic nanoscaffolds to culture osteoblast cells on, and investigate specific responses of the cells towards the scaffolds. Osteoclast-like cells and osteoblast cells were cultured separately on the nanoscaffolds and the proliferation, adhesion and cellular response were determined. In this study, the mineralisation of the osteoblast cells was observed in a time study. The intracellular calcium ion concentration and nitric oxide concentration were determined in vitro while the cells were proliferating on the scaffolds. The expression of endothelial and inducible nitric oxide synthase was determined immunohistochemically. Quantitative data were obtained from fluorometer studies. Qualitative data was supplied by light-and fluorescent confocal microscopy. During studies with microscopy, a minimum of five representative images from each sample were captured. The cells showed increased mineralisation over time. An increase in intracellular Ca 2+ was not observed when compared to the controls. However, an increase in intracellular nitric oxide formation was detected. Expression of endothelial nitric oxide synthase but not inducible nitric oxide synthase was detected in vitro. From the results we can conclude that the scaffolds are biocompatible and conducive to healthy cell growth and differentiation and could possibly be applied in non-load-bearing bone regeneration and repair applications.

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“…This investigation has been conducted for different case studies for the mice bones. The analogous diversity is also found in case of zinc, which provides exceptional bone growth and as it is known to be deposit between zones of bone ( Hooshmand et al, 2014 , Ibrahim et al, 2016 ). The diversity of gathering of both tungsten and zinc at the inner and outer edges of the bone tissue is likely to be linked to the variation of width, and thus enhanced remodelling activity, of the long bone in the case of young, growing mice.…”

Section: Introductionmentioning

confidence: 65%

Study of accumulation behaviour of tungsten based composite using electron probe micro analyser for the application in bone tissue engineering

Chen

Guo³

et al. 2020

Saudi Journal of Biological Sciences

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In this research, a proto-type study we have conducted, where we have synthesized tungsten based composite materials which are tungsten along with combined oxides of other elements like calcium, scandium, barium, and aluminium in the form of powder with bones powder of mice devised by high energy ball mill and later on fabricating high dense pellets by sintering by spark plasma. The particle sizes of the composite materials are found to be 1–2 µm, as evidenced by the electron microscope, suggesting synthesized materials are of micron size. The quantitative and qualitative analysis of sintered pellets are well confirmed by electron probe micro analyzer (EPMA) and energy dispersive X-ray spectrometer (EDS) which illustrate the greater percentage of tungsten presents in the profound scan areas with other elements of the composite. The absence of pores across the 3D geometry suggesting dense sample, which is quite revealed by the X-ray tomography inspection. The prepared sintered pellets from the tungsten based composites are found to be ≈ 99.5% density with the observation of tungsten to be accumulated uniformly across the scan regions along with focussed hot spots as implied by EPMA. This study paves the way, to examine how the tungsten accumulation and the distribution with the other elements for future understanding in bone tissue engineering application and the in vivo specification of tungsten.

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Bioactivity studies and adhesion of human osteoblast (hFOB) on silicon-biphasic calcium phosphate material

Cited by 24 publications

References 21 publications

Polymerizing Pyrrole Coated Poly (l-lactic acid-co-ε-caprolactone) (PLCL) Conductive Nanofibrous Conduit Combined with Electric Stimulation for Long-Range Peripheral Nerve Regeneration

Polymerizing Pyrrole Coated Poly (l-lactic acid-co-ε-caprolactone) (PLCL) Conductive Nanofibrous Conduit Combined with Electric Stimulation for Long-Range Peripheral Nerve Regeneration

hFOB 1.19 osteoblast cells grown on a biomimetic biphasic nanoscaffold: An in vitro evaluation for possible bone tissue engineering

Study of accumulation behaviour of tungsten based composite using electron probe micro analyser for the application in bone tissue engineering

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