Bioactivity screening by chromatography-bioluminescence coupling

Eberz, Günther; Rast, H.G.; Bürger, K.; Kreiss, Wolfgang; Weisemann, Claus

doi:10.1007/bf02272814

Cited by 56 publications

(24 citation statements)

References 4 publications

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“…14 The detection of bioactive compounds in complex samples by bacterial assays using the directly bioluminescent marine bacteria Vibrio fischeri has been reported. [15][16][17] Many enzyme substrate reactions that show the viability of microorganism are suited for use in HPTLC bioassay. For instance, in the detection of algicides 18 and fungicides.…”

Section: Microbial Assaymentioning

confidence: 99%

Bioassay-coupled Chromatographic Analysis of Medicinal Natural Products: A Review

Elufioye¹

2017

TJNPR

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Section: Microbial Assaymentioning

confidence: 99%

Bioassay-coupled Chromatographic Analysis of Medicinal Natural Products: A Review

Elufioye¹

2017

TJNPR

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“…This tool was recently advanced by the introduction of automated multiple development (AMD) enabling the use of solvent gradients [70,71]. TLC allows a direct combination with simple biotests such as bioluminescence inhibition of Vibrio fischeri by subsequent coating of the plates with the bacteria.…”

Section: Aqueous Samplesmentioning

confidence: 99%

Effect-directed analysis: a promising tool for the identification of organic toxicants in complex mixtures?

Brack

2003

Analytical and Bioanalytical Chemistry

395

238

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Wastewater effluents, groundwater, surface water, sediments, soils and air particulate matter are often contaminated by a multitude of chemicals. Since often no a priori knowledge of relevant toxicants exists, chemical analysis alone is not an appropriate tool for hazard assessment. Instead, a linkage of effect data and hazardous compounds is required. For that purpose, effect-directed analysis (EDA) was developed, which is based on a combination of biotesting, fractionation procedures and chemical analytical methods. Since a controversial discussion about the prospects of success in relation to the expense exists, the current methodological state of EDA for organic toxicants in complex mixtures and important results are reviewed in this paper with the aim of establishing criteria for the successful use of this promising tool. While EDA is a powerful tool to identify specifically acting individual toxicants close to the source of emission, it is inappropriate for screening purposes and often may fail in remote areas where the concentrations of specific toxicants are too low relative to the nonspecific toxicity of the whole mixture of natural and anthropogenic compounds. The biological tools have to be carefully selected with respect to their ability to detect specific effects and their significance in hazard assessment. Sophisticated chemical tools are required to identify individual toxicants in mixtures of thousands of compounds, which are typical for contaminated environments.

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“…When these bacteria are exposed to poisonous substances, the metabolic activity is affected, resulting in a proportional decrease in luminescence. This phenomenon can be exploited in TLC bioautographic assays to detect natural toxins (Eberz et al, 1996;Sherma, 2010). Even if this bioassay involves bacteria, it cannot be considered as an actual antimicrobial bioautographic test, as it does not show a genuine growth inhibition but rather a disruption of the respiratory chain.…”

Section: Toxicity Bioautographic Assaysmentioning

confidence: 99%

Thin‐layer Chromatography of Plants, with Chemical and Biological Detection Methods

Urbain

Simões-Pires

2014

Encyclopedia of Analytical Chemistry

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Thin‐layer chromatography ( TLC ) is a widespread separation technique in the field of plant analysis and offers multiple advantages. This versatile technique can provide useful information about the qualitative or semiquantitative chemical composition of a mixture, thanks to coupling, with the detection and spectroscopic techniques and devices for image treatment. Moreover, it can contribute to the detection of biological activity by combining chromatography with bioassays, such as antimicrobial or enzyme inhibition tests. This procedure, providing simultaneous chromatographic separation and in situ assessment of a given biological activity, is named TLC bioautography. The major advantage of this technique is the ability to locate active substances from a complex mixture without prior sample purification. This avoids time‐consuming and tedious isolation of already‐known or inactive compounds. As this method is simple and cost effective, it is suitable for preliminary rapid screening to detect potential activity, as well as being a useful monitoring tool to track the bioguided isolation of active components. In this chapter, we focus on up‐to‐date TLC bioautographic assays, detail their mechanisms and applications in the field of natural drug discovery, and discuss the recent advances of this method that might appear at first glance to be a trivial approach.

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Bioactivity screening by chromatography-bioluminescence coupling

Cited by 56 publications

References 4 publications

Bioassay-coupled Chromatographic Analysis of Medicinal Natural Products: A Review

Bioassay-coupled Chromatographic Analysis of Medicinal Natural Products: A Review

Effect-directed analysis: a promising tool for the identification of organic toxicants in complex mixtures?

Thin‐layer Chromatography of Plants, with Chemical and Biological Detection Methods

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