Bioactivity of nanostructure on titanium surface modified by chemical processing at room temperature

Komasa, Satoshi; Taguchi, Yoichiro; Nishida, Hisataka; Tanaka, Masahiro; Kawazoe, Takayoshi

doi:10.1016/j.jpor.2011.12.002

Cited by 37 publications

(60 citation statements)

References 30 publications

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“…30 The nanotopography of nanonetwork structures with TNS nanofeatures is affected by temperature, treatment time, and alkaline concentration. 20,[31][32][33] The objectives of the present study were twofold: first, we aimed to investigate the changes in TNS, such as roughness, wettability, and surface structure induced by various alkaline concentrations (2.5, 5.0, 7.5, 10.0, and 12.5 M), and second, we aimed to evaluate the influence of such modified nanonetwork structures with TNS nanofeatures, with different alkaline concentrations, on the osteogenic differentiation of rat BMMSCs.…”

Section: Introductionmentioning

confidence: 99%

Osteogenic activity of titanium surfaces with nanonetwork structures

Huan¹,

Komasa²,

Taguchi³

et al. 2014

IJN

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Background Titanium surfaces play an important role in affecting osseointegration of dental implants. Previous studies have shown that the titania nanotube promotes osseointegration by enhancing osteogenic differentiation. Only relatively recently have the effects of titanium surfaces with other nanostructures on osteogenic differentiation been investigated. Methods In this study, we used NaOH solutions with concentrations of 2.5, 5.0, 7.5, 10.0, and 12.5 M to develop a simple and useful titanium surface modification that introduces the nanonetwork structures with titania nanosheet (TNS) nanofeatures to the surface of titanium disks. The effects of such a modified nanonetwork structure, with different alkaline concentrations on the osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMMSCs), were evaluated. Results The nanonetwork structures with TNS nanofeatures induced by alkali etching markedly enhanced BMMSC functions of cell adhesion and osteogenesis-related gene expression, and other cell behaviors such as proliferation, alkaline phosphatase activity, extracellular matrix deposition, and mineralization were also significantly increased. These effects were most pronounced when the concentration of NaOH was 10.0 M. Conclusion The results suggest that nanonetwork structures with TNS nanofeatures improved BMMSC proliferation and induced BMMSC osteogenic differentiation. In addition, the surfaces formed with 10.0 M NaOH suggest the potential to improve the clinical performance of dental implants.

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Section: Introductionmentioning

confidence: 99%

Osteogenic activity of titanium surfaces with nanonetwork structures

Huan¹,

Komasa²,

Taguchi³

et al. 2014

IJN

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“…In a recent work, we demonstrated that TiO 2 nanotubes and TNSs could be formed on titanium metal surfaces by treatment with a 10 M NaOH aqueous solution at 30°C, and we used this method to prepare TNS-modi ed disks. Komasa et al [8] suggested that TNSs on titanium surfaces can be applied to control the osteogenic di erentiation of bone marrow cells and enhance mineralization. Our results demonstrated that TNS-modi ed titanium disks were more hydrophilic and showed uniquely enhanced wettability in comparison with unmodi ed disks.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous study [8], we showed that TNSs created by compound processing enhance the osteogenic separation of rodent bone marrow (RBM) cells. e surface properties and structural characteristics of materials play an important role in protein and cell adsorption behaviors.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Titania Nanosheet Adsorption Behavior Using a Quartz Crystal Microbalance Sensor

Tashiro

Komasa

Miyake

et al. 2018

Advances in Materials Science and Engineering

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We investigated the adsorption of albumin and fibronectin on a titania nanosheet-(TNS-) modified quartz crystal microbalance (QCM) sensor. A Ti QCM sensor was fabricated by reactive magnetron sputtering. A thin layer of Ti was deposited on the QCM sensor. is sensor was then alkali-modified by treatment with NaOH at room temperature to fabricate the titania nanosheets. Scanning probe microscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy were performed to investigate the surface topology and chemical components of each sensor. e TNS had a titanium oxide film exhibiting a nodular structure and a thickness of 13 nm on the QCM sensor. Furthermore, QCM measurements showed significantly greater amounts of albumin and fibronectin adsorbed on the TNS than on titanium. e NaOH treatment of titanium modified the sensor surface and improved the adsorption behaviors of proteins related to the initial adhesion of bone marrow cells. erefore, we concluded that TNS improves the initial adhesion between the implant materials and the surrounding tissues.

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“…It has also been shown that treatment with NaOH aqueous solution produces a rough, nanoscale surface. A previous study reported that TNS produced via chemical processing promoted osteogenic differentiation of rat bone marrow (RBM) cells 4,5) . The surface properties and structures of materials play important roles in the adsorption of proteins, which might influence cell behavior.…”

Section: Introductionmentioning

confidence: 99%

“…24(3):257 -266, 2015 reports that NaOH treatment leads to the formation of a Ti-O-Na titanate layer on the Ti surface 4,6) . V is known to exhibit biological toxicity, so it is interesting that this component was undetected on the TNS-modified titanium surface.…”

Section: Introductionmentioning

confidence: 99%

Bioactivity of Titanium Surface Nanostructures Following Chemical Processing and Heat Treatment

Komasa

Taguchi

et al. 2015

J. Hard Tissue Biology.

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Our previous research reports that NaOH treatment leads to the formation of a Ti-O-Na titanate layer on the titanium-6-aluminium-4-vanadium (Ti6Al4V) surface. However, this titanium nanosheets (TNS) hydrogel layer is so brittle that it easily detaches from the implant and can cause many problems, including degradation in the living body due to inhomogeneous composition distribution. The aims of the present study were to investigate combined alkali-treatment of Ti6Al4V alloy and then heated and evaluate the ability of this modified surface to affect osteogenic differentiation of rat bone marrow (RBM) cells, to increase the success rate of titanium implants. We fabricated TNS on titanium alloy surfaces by NaOH treatment prior to heat treatment at 600 °C, and determined RBM cell properties and differentiation potential on the surface in comparison to untreated control surfaces. The nanoscale network structures formed by alkali etching markedly enhanced RBM cell adhesion and osteogenic-related gene expression. Other cell behaviors, such as proliferation, alkaline phosphatase activity, osteocalcin deposition and mineralization, were markedly increased on the TNS-modified Ti6Al4V with heat treatment. Our results suggest that titanium implants modified with nanostructures promote osteogenic differentiation, which may improve the biointegration of these implants into the alveolar bone.

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Bioactivity of nanostructure on titanium surface modified by chemical processing at room temperature

Abstract: In conclusion, these data suggest that titanium implants modified by the application of nanostructures promote osteogenic differentiation, and may improve the biointegration of these implants into the alveolar bone.

Cited by 37 publications

References 30 publications

Osteogenic activity of titanium surfaces with nanonetwork structures

Osteogenic activity of titanium surfaces with nanonetwork structures

Analysis of Titania Nanosheet Adsorption Behavior Using a Quartz Crystal Microbalance Sensor

Bioactivity of Titanium Surface Nanostructures Following Chemical Processing and Heat Treatment

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