Bioactivity of Acanthus mollis – Contribution of benzoxazinoids and phenylpropanoids

Matos, Patrícia; Figueirinha, Artur; Paranhos, António; Nunes, Frederico; Cruz, Pedro F.; Geraldes, Carlos F. G. C.; Cruz, M.T.; Batista, Maria Teresa

doi:10.1016/j.jep.2018.09.013

Cited by 14 publications

(16 citation statements)

References 44 publications

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“…In Sardinia, Spain, and Portugal, the leaves are boiled and applied in a poultice as an emollient or as skin protection. They are also recommended for the treatment of wounds and burns, hemorrhoids, toothache, and inflammation of the oral cavity [21]. To date, several pharmacological studies have reported the anti-inflammatory and anti-hemorrhoidal capacity of A. mollis leaves, as well as the antifungal and antioxidant capacity of certain extracts [22] The purpose of this study was to analyze neuroprotective and free radical scavenging capacity through in vitro and in cell culture assays of verbascoside isolated from A. mollis leaves.…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective Potential of Verbascoside Isolated from Acanthus mollis L. Leaves through Its Enzymatic Inhibition and Free Radical Scavenging Ability

Burgos,

Muñoz-Mingarro,

Navarro

et al. 2020

Antioxidants

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The phenomenon of today’s ageing population has increased interest in the search for new active substances that delay the onset and development of neurodegenerative diseases. In this respect, the search for natural compounds, mainly phenolic compounds, with neuroprotective activity has become the focus of growing interest. Verbascoside is a phenylethanoid that has already presented several pharmacological activities. The purpose of this study is to isolate and identify verbascoside from Acanthus mollis leaves. Consequently, its neuroprotective ability through enzymatic inhibition and free radical scavenging ability has been analyzed both in vitro and in cell culture assays. The antioxidant capacity of verbascoside was evaluated in vitro through total antioxidant capacity, DPPH•, OH•, and O2•—scavenging activity assays. The effect of verbascoside on intracellular reactive oxygen species (ROS) levels of HepG2 and SH-SY5Y cell lines was studied in normal culture and under induced oxidative stress. The inhibitory ability of the phenylethanoid against several enzymes implied in neurodegenerative diseases (tyrosinase, MAO-A, and AChE) was analyzed in vitro. Verbascoside neuroprotective activity is at least in part related to its free radical scavenging ability. The effect of verbascoside on ROS production suggests its potential in the prevention of harmful cell redox changes and in boosting neuroprotection.

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Section: Introductionmentioning

confidence: 99%

Neuroprotective Potential of Verbascoside Isolated from Acanthus mollis L. Leaves through Its Enzymatic Inhibition and Free Radical Scavenging Ability

Burgos,

Muñoz-Mingarro,

Navarro

et al. 2020

Antioxidants

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“…In the EtOAc chromatogram ( Figure 1 B), peak 8 was identified as p -coumaric acid. Peaks 9 and 11 with wavelength maxima at 272 and 335 was characteristic of apigenin derivatives [ 9 ]. Peak 11 was identified as isovitexin with a commercial standard.…”

Section: Resultsmentioning

confidence: 99%

Vascular Effects of Polyphenols from Agrimonia eupatoria L. and Role of Isoquercitrin in Its Vasorelaxant Potential in Human Arteries

et al. 2022

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Agrimonia eupatoria L. has been traditionally used for the treatment of inflammatory diseases but also as a hypotensive. To our knowledge, only one study has previously suggested an improvement in vascular endothelial function in diabetic conditions, as the underlying mechanisms and responsible compounds are unknown. In this study, we aimed to assess the direct vascular effects of Agrimonia eupatoria L. in human arteries. The infusion elicited a mild increase in basal vascular tone and a significant potentiation of the adrenergic contraction of 49.18% at 0.02 mg/mL, suggesting the presence of compounds with mild vasoconstrictor activity. In contrast, the ethyl acetate fraction inhibited adrenergic contraction by 80.65% at 2 mg/mL and elicited no effect on basal vascular tone. A potent concentration-dependent vasorelaxation was observed for both the infusion and the ethyl acetate fraction (maximal relaxation above 76% and 47%, respectively). Inhibition of nitric oxide synthase and cyclooxygenase elicited significant decreases in the vasorelaxation to the infusion, as, for the ethyl acetate fraction, only the cyclooxygenase pathway appeared to be involved. Isoquercitrin elicited a vasoactivity consistent with the ethyl acetate fraction, suggesting this is a major component responsible for the vasorelaxant properties of A. eupatoria. Further research is warranted to fully evaluate its vasoprotective properties with therapeutic potential in several conditions, e.g., atherosclerosis.

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“…Reported analytical methods for estimation of benzoxazinoid class of compound included either phosphate buffer (pH 2.4) and methanol or 5% aqueous formic acid solution and methanol as mobile phase in gradient mode [16,17], which could not be able to resolve the peak of blepharin from sample solution prepared from seeds of B.persica . A new HPLC method for quantitative estimation of blepharin was, thus, developed initially by replacing the methanol with acetonitrile in the mobile phase, then adjusting the proportion of acetonitrile for better separation as well as optimizing the pH of water for getting gaussian shape of the blepharin.…”

Section: Resultsmentioning

confidence: 99%

“…HPLC method for quantitative estimation of benzoxazinoid glycoside (blepharin) was developed by modifying the reported analytical method for determination and estimation of benzoxazinoid class of compound [16,17]. The chromatographic conditions were optimized through trial and error approach, to get a well‐resolved peak of blepharin having RT less than 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…Benzoxazinoids were identified using Ultra performance liquid chromatography‐Mass and HPLC–PDA detector based analytical method was developed to estimate benzoxazinoids in wheat and rye beers [16]. Quantification of benzoxazinoids from ethanol extracts of Acanthus mollis leaves using HPLC‐Photodiode array detector (PDA) method was also reported [17]. The literature survey revealed an absence of the HPLC method for the estimation of benzoxazinoid glycoside from this biologically active plant, which would be of immense help in standardizing this plant drug.…”

Section: Introductionmentioning

confidence: 99%

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Isolation, characterization and estimation of benzoxazinoid glycoside from seeds of Blepharis persica (Burm.f) O. Kuntze

Gaikar

Raval

Patel

et al. 2021

Separation Science Plus

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Seeds of Blepharis persica, the extracts of which have various pharmacological activities, contain benzoxazinoid compounds. The present studies were aimed to isolate benzoxazinoid class of compound using chromatography, identify the isolated compound using spectroscopic methods, and development of a high‐performance liquid chromatography‐based analytical method for estimation of the constituent. The compound was isolated using HP‐20 and silica‐based column from n‐butanol fraction of Blepharis persica seeds and identified as blepharin from interpretation of spectra. An analytical method was developed using high‐performance liquid chromatography containing a photodiode‐array detector and validated as per ICH Q2(R1) guideline for estimation of blepharin. Blepharin was resolved using a C18 column, whereas the optimized mobile phase was water (pH 8): acetonitrile (90:10, %v/v). The linearity was established between 0.5 to 10.00 μg/mL. Limit of detection and limit of quantification were 0.015 and 0.046 μg/mL, respectively. The recovery was in the range of 100.9‐103.9%. The method was found to be selective, precise, and robust too. The content of blepharin was found (0.708 ± 0.02)% w/w of the dried weight basis of plant extract.

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Bioactivity of Acanthus mollis – Contribution of benzoxazinoids and phenylpropanoids

Cited by 14 publications

References 44 publications

Neuroprotective Potential of Verbascoside Isolated from Acanthus mollis L. Leaves through Its Enzymatic Inhibition and Free Radical Scavenging Ability

Neuroprotective Potential of Verbascoside Isolated from Acanthus mollis L. Leaves through Its Enzymatic Inhibition and Free Radical Scavenging Ability

Vascular Effects of Polyphenols from Agrimonia eupatoria L. and Role of Isoquercitrin in Its Vasorelaxant Potential in Human Arteries

Isolation, characterization and estimation of benzoxazinoid glycoside from seeds of Blepharis persica (Burm.f) O. Kuntze

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