Bioactivity-Guided Investigation of the Anti-Inflammatory Activity of Hippophae rhamnoides Fruits

Abstract

According to modern ethnobotanical records, the fruit of is effective in the treatment of different allergic symptoms. In order to obtain pharmacological evidence for this observation, the fruit was investigated for anti-inflammatory activity using animal models. Aqueous and 70% MeOH extracts were tested in 48/80-induced rat paw edema assay after oral administration, and it was found that the 70% MeOH extract (500 mg/kg) reduced significantly edema volume (0.660 ± 0.082 mL vs. control 0.935 ± 0.041 mL). Extracts of different parts of the fruit (pulp, peel, seed) were investigated in the same assay, and the peel extract was shown to exhibit maximum edema-reducing effect (0.470 ± 0.124 mL vs. control 0.920 ± 0.111 mL). This extract was used to elucidate the mode of action. Different inflammation inducers (serotonin, histamine, dextran, bradykinin, and carrageenan) were applied in the rat paw model, but the extract inhibited only the compound 48/80 elicited inflammation. The active extract was then fractionated by solvent-solvent partitioning and chromatographic methods with the guidance of the 48/80-induced anti-inflammatory assay, and the main compounds responsible for the activity were identified as ursolic acid and oleanolic acid. Our data suggest that the activity is most probably based on a membrane stabilizing effect caused by the inhibition of degranulation of mast cells. Moreover, previously unknown 2,5-bis-aryl-3,4-dimethyltetrahydrofuran lignans, nectandrin B, fragransin A, and saucernetindiol were isolated and identified from for the first time.

Ursolic Acid Inhibits Tumor Growth <i>via</i> Epithelial-to-Mesenchymal Transition in Colorectal Cancer Cells

“…8) Ursolic acid (UA; 3β-hydroxyurs-12-en-28-oic acid), a pentacyclic triterpenic acid, is widely existing in edible plants and medicinal materials. [9][10][11] A lot of traditional Chinese medicinal herbs containing this compound, such as Hedyotic diffusa, 12) Radix actinidiae, 13) and Hippophae rhamnoides LINN., 14) are frequently used to treat colorectal cancer in China, nonetheless the mechanisms underlying their effects are still unclear. UA shows a variety of biological effects that are likely to influence cancer development, including antiviral, anti-inflammatory, antioxidant, hepatoprotective, anti-tumor, cytotoxic, anti-metastatic activities, and anti-angiogenetic.…”

Lignans, Amides, and Saponins from Haplophyllum tuberculatum and Their Antiprotozoal Activity

“…The two tetrahydrofuran-type lignans 1 and 2 were previously reported from different Myristicaceae, Elaeagnaceae, Poaceae, and Piperaceae species [22,33].…”

The bioactive components as well as the nutritional and health effects of sea buckthorn

“…38,39 3 The nutritional and health effects of SB 3.1 Anti-inammatory and immunomodulatory effects 3.1.1 Anti-inammatory effect. SB extract or preparation as well as the isolated component was found to possess anti-inammatory activity, thus may be used for the prevention or treatment of periodontal inammation, 40 oropharyngeal mucositis, 41 skin inammatory disease, 42,43 endotoxin induced sepsis 44 and allergic symptoms, 45 etc. Isorhamnetin, a avonoid abundant in the fruit of SB, showed a variety of anti-inammatory activities.…”

“…Isorhamnetin, a avonoid abundant in the fruit of SB, showed a variety of anti-inammatory activities. [46][47][48][49] Besides ursolic acid and oleanolic acid, 45 two well-known anti-oxidant and anti-inammatory agents widely applied in cosmetics, casuarinin was also showed potent anti-inammatory property. 50 The detailed parameters of experiments including experimental models, dosage and possible mechanisms were listed in ESI Tab.…”