Bioactivity and Thermal Stability of Collagen–Chitosan Containing Lemongrass Essential Oil for Potential Medical Applications

Râpă, Maria; Zaharescu, Traian; Stefan, Laura; Gaidău, Carmen; Stănculescu, Ioana; Constantinescu, Rodica Roxana; Stanca, Maria

doi:10.3390/polym14183884

Cited by 10 publications

(9 citation statements)

References 59 publications

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“…The FTIR spectra of gelatins compared with the nanofiber spectra showed irregular variations in intensity and shape due to the inherent difficulty of sample analysis as a consequence of intrinsic solid material hardness and local collagen structures. Amide A, amide B, amide I, amide II, and amide III bands characteristic of collagenbased materials are given in Table 3, being in good agreement with previous data [37][38][39]. The shifts to lower wavenumbers observed in Table 3 correlate with participation in hydrogen bonds [56].…”

Section: Atr-ftirsupporting

confidence: 89%

“…The shifts to lower wavenumbers observed in Table 3 correlate with participation in hydrogen bonds [56]. Band position modifications are also due to different amino acid Amide A, amide B, amide I, amide II, and amide III bands characteristic of collagenbased materials are given in Table 3, being in good agreement with previous data [37][38][39]. The shifts to lower wavenumbers observed in Table 3 correlate with participation in hydrogen bonds [56].…”

Section: Atr-ftirsupporting

confidence: 88%

“…It was reported that the collagen membranes fabricated by casting blends of gelatin with 0.7% lemongrass essential oils can be preserved against oxidation induced by gamma irradiation radicals compared to membranes without essential oils [37]. Also, the influence of riboflavin on different-origin gelatins subjected to gamma irradiation showed the retention of free radicals on oxygen and modification of the secondary structure of the protein.…”

Section: Introductionmentioning

confidence: 99%

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The Influence of Gamma Radiation on Different Gelatin Nanofibers and Gelatins

Gaidau,

Râpă,

Ionita

et al. 2024

Gels

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Gelatin nanofibers are known as wound-healing biomaterials due to their high biocompatible, biodegradable, and non-antigenic properties compared to synthetic-polymer-fabricated nanofibers. The influence of gamma radiation doses on the structure of gelatin nanofiber dressings compared to gelatin of their origin is little known, although it is very important for the production of stable bioactive products. Different-origin gelatins were extracted from bovine and donkey hides, rabbit skins, and fish scales and used for fabrication of nanofibers through electrospinning of gelatin solutions in acetic acid. Nanofibers with sizes ranging from 73.50 nm to 230.46 nm were successfully prepared, thus showing the potential of different-origin gelatin by-products valorization as a lower-cost alternative to native collagen. The gelatin nanofibers together with their origin gelatins were treated with 10, 20, and 25 kGy gamma radiation doses and investigated for their structural stability through chemiluminescence and FTIR spectroscopy. Chemiluminescence analysis showed a stable behavior of gelatin nanofibers and gelatins up to 200 °C and increased chemiluminescent emission intensities for nanofibers treated with gamma radiation, at temperatures above 200 °C, compared to irradiated gelatins and non-irradiated nanofibers and gelatins. The electron paramagnetic (EPR) signals of DMPO adduct allowed for the identification of long-life HO● radicals only for bovine and donkey gelatin nanofibers treated with a 20 kGy gamma radiation dose. Microbial contamination with aerobic microorganisms, yeasts, filamentous fungi, Staphylococcus aureus, Escherichia coli, and Candida albicans of gelatin nanofibers treated with 10 kGy gamma radiation was under the limits required for pharmaceutical and topic formulations. Minor shifts of FTIR bands were observed at irradiation, indicating the preservation of secondary structure and stable properties of different-origin gelatin nanofibers.

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Section: Atr-ftirsupporting

confidence: 89%

Section: Atr-ftirsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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The Influence of Gamma Radiation on Different Gelatin Nanofibers and Gelatins

Gaidau,

Râpă,

Ionita

et al. 2024

Gels

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“…Other papers related to the valorization of collagen extracted from animal by-products for medical applications have reported a good biocompatibility [23,24,67,68]. Similarly, Ramirez et al [69] successfully developed two fibrous layers using polyvinyl alcohol (PVA) and wool-keratin for wound healing purposes, demonstrating a noteworthy enhancement of the in vitro cell adhesion.…”

Section: In Vitro Cytocompatibilitymentioning

confidence: 98%

Conversion of Animal-Derived Protein By-Products into a New Dual-Layer Nanofiber Biomaterial by Electrospinning Process

Gaidău,

Râpă,

Stefan

et al. 2023

Fibers

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The aim of this study was to design a dual-layer wound dressing as a new fibrous biomaterial based on the valorization of animal-derived proteins. The first layer was fabricated by the deposition of poly(ethylene oxide) (PEO) loaded with keratin hydrolysate (KH) via a mono-electrospinning process onto a poly(lactic acid) (PLA) film, which was used as a support. The second layer consisted of encapsulating a bovine collagen hydrolysate (CH) into poly(vinyl pyrrolidone) (PVP) through a coaxial electrospinning process, which was added onto the previous layer. This assemblage was characterized by electronic microscopy for morphology and the controlled release of KH. In vitro biocompatibility was evaluated on the L929 (NCTC) murine fibroblasts using quantitative MTT assay and qualitative cell morphological examination after Giemsa staining. Additionally, in vivo biocompatibility methods were used to assess the impact of the biomaterial on white Swiss mice, including the evaluation of hematological, biochemical, and immunological profiles, as well as its impact on oxidative stress. The results revealed a nanofibrous structure for each layer, and the assembled product demonstrated antioxidant activity, controlled release of KH, a high degree of in vitro biocompatibility, negligible hematological and biochemical changes, and minimal impact of certain specific oxidative stress parameters compared to the use of patches with textile support.

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“…The authors in [ 71 ] produced chitosan films with Hypericum perforatum EOs and verified that these formulations were good surfaces for cell attachment. Moreover, various studies mention that films with EOs and PEs are biocompatible [ 78 , 82 , 83 , 86 , 87 ], presenting low cytotoxicity [ 79 ].…”

Section: Formulations Incorporated With Eos and Pesmentioning

confidence: 99%

Plant-Based Films and Hydrogels for Wound Healing

Lopes,

Pintado,

Tavaria

2024

Microorganisms

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Skin is constantly exposed to injury and infectious agents that can compromise its structural integrity and cause wounds. When this occurs, microorganisms from the skin microbiota and external bacteria and fungi can penetrate the wound and cause an infection, which complicates the healing process. Nowadays, there are several types of wound dressings available to treat wounds, some of which are incorporated with antimicrobial agents. However, the number of microorganisms resistant to these substances is rising. Therefore, the search for new, natural alternatives such as essential oils (EOs) and plant extracts (PEs) is on the rise. However, these substances present some limitations (poor bioavailability and poor target capacity), which limits their efficiency. Their incorporation in formulations in the form of films and hydrogels (HGs) can help to overcome these issues and may be a potential alternative to the current treatments. HGs and films incorporated with PEs and EOs have antimicrobial activity, promote the viability of skin cells and fibroblast migration, and are non-toxic and biocompatible. This review discusses the use of films and HGs for the topical delivery of EOs and PEs for wound treatment and their formulations as effective wound dressings, while debating some mechanisms and biological properties to elucidate their presumptive clinical relevance and possible applications.

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Bioactivity and Thermal Stability of Collagen–Chitosan Containing Lemongrass Essential Oil for Potential Medical Applications

Cited by 10 publications

References 59 publications

The Influence of Gamma Radiation on Different Gelatin Nanofibers and Gelatins

The Influence of Gamma Radiation on Different Gelatin Nanofibers and Gelatins

Conversion of Animal-Derived Protein By-Products into a New Dual-Layer Nanofiber Biomaterial by Electrospinning Process

Plant-Based Films and Hydrogels for Wound Healing

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