Bioactivity and DNA/BSA Interactions of Selenium N‐Heterocyclic Carbene Adducts

Abstract

In wake of promising biomedical applications of organo‐selenium compounds, the present work was planned to synthesize selenium adducts (1 s‐‐4 s) and their respective azolium ligands (1 a–4 a), which were subsequently authenticated by various analytical and spectroscopic techniques like microanalysis, UV‐Visible, FT‐IR, 1H & 13C NMR and Electrospray Ionisation‐Mass Spectrometry (ESI‐MS). Moreover, the synthesized compounds were tested for their biological potential like antioxidant, antimicrobial, hemolytic, DNA and Bovin Serum Albumin (BSA) interactions, stability and lipophilicity using in‐vitro models. All the compounds were found stable in solution form for studied period 12–68 hours and showed good lipophilic values in the range of logP 0.66–0.85. In context to biological activities, selenium compounds showed better antimicrobial (ranging 17.4±0.5–25.3±0.8 mm against S. aureus and 18.6±0.7–24.1±0.9 against E.coli at concentration of 0.3 mg/mL for both strains) and antioxidant potential (IC50 ranging 40.0–62.0 μg/mL for DPPH solutions) compared to respective ligands. The interaction study of compounds (1 s–4 s) to DNA and BSA showed prominent binding affinity.

Synthesis, characterization and biological evaluation of Bis-benzimidazolium salts and their silver(I)-N-heterocyclic carbene complexes

“…Tritone-x was used as a positive control and PBS as the negative control. On the plate reader of ELISA, measurement of absorption was taken at 570 nm [54].…”

“…% Hemolytic = [(Abs of sample -Abs of negative control)/Abs of positive control] × 100 4.5. Antioxidant Activity Antioxidant potentials of newly synthesized salts and complexes were studied by the DPPH method [54] with slight alterations. Test solutions were made with a concentration of 10 mg/mL in DMSO.…”

Synthesis, structural studies and computational evaluation of cyclophanes incorporating imidazole-2-selones

“…18 Many imidazole-2-selones are known. 12,32,[34][35][36][37][38][39][40][41][42] However, there are no reports of cyclophanes incorporating imidazole-2-selone moieties. As an extension of our study with imidazole-2-thiones, herein, we report the rst synthesis of some cyclophanes, including imidazole-2-selone moieties, and study their conformational behaviour in solution and the solid state as well as use DFT calculations.…”