Bioactivity and cell viability of Ag+- and Zr4+-co-doped biphasic calcium phosphate

Biphasic calcium phosphate doped with zirconia nanoparticles for reconstruction of induced mandibular defects in dogs: cone-beam computed tomographic and histopathologic evaluation

“…X-ray diffraction analysis demonstrated that the development of zirconia phase had an impact on the proportion of the TCP/HA owing to the exchange of the larger radius ion of the Ca 2+ (0.099 nm) by the smaller one of the Zr 4+ (0.084 nm) in agreement with previous studies [ 17 , 20 ] concluded that by increasing the TCP/HA proportion, their reactivity is intensified. El-Bassyouni et al specified that such doping process was capable to improve the physicochemical, morphological and size parameters of the prepared material [ 21 ].…”

“…Osteoinductivity could be achieved by adding dopants materials to HA [ 30 ]. In the current study, doping of TCP/HA with zirconia resulted in statistically significant increase in bone radiodensity compared to TCP/HA which could be attributed to the role of zirconia in increasing the mechanical properties (compressive strength and stiffness) as previously reported [ 17 ].…”

“…Cell mode death supported the significance of Zr-doping. The increased viability of tested samples increases the likelihood of the materials to be applied in restoration of the damaged bone sites [ 17 ].…”

“…The resulting precipitate was washed several times using deionized water before being filtered. The obtained powder was dried for 5 h at 100 °C then calcined in an oven for 2 h at 900 °C [ 17 ]. Powder was sieved into fine form of <0.037 mm and shaped into discs of 15 mm diameter using uniaxial pressure (20KN) and (7%) PVA solution as a binder.…”

“…At the end of the incubation periods, all slides were washed with PBS, stained by ethidium bromide/acridine orange for 10 min then microscopically inspected via fluorescence microscope. The cytocompatibility information were published elsewhere [ 17 ].…”