Bioactivities of phenolic blend extracts from Chilean honey and bee pollen

Abstract

It is known that polyphenolic extracts have antibacterial activity, but usually with not very broad spectrum of action. To enhance its individual antibacterial effect, to combine different extracts has been proposed. In this work, the combined antibacterial effect of 3 extracts and their mixtures (Ulmo and Quillay honeys, multifloral pollen) was evaluated. Their polyphenol content was quantified by HPLC-DAD analysis. E. coli, S. aureus, Salmonella spp, Pseudomonas spp, L. acidophilus, and B. thermosphacta were used as foodborne pathogenic-spoilage strains in the antibacterial assays. Polyphenol-target bacteria relationships were established. Results showed that all extracts and their combinations have activity against all strains. Relationships were established between all pathogenic strains with 4 phenolic acids and 2 flavonoids, and 2 spoilage strains with 3 phenolic acids and 3 flavonoids. These findings will contribute to the rapid assessment of mixtures of outstandingly antibacterial extracts to be applied in the food industry.Bioactividad de mezclas de extractos fenólicos obtenidos de mieles y polen de abeja chilenos RESUMEN Se sabe que los extractos polifenólicos tienen actividad antibacteriana con un espectro de acción generalmente no muy amplio. Para potenciar su efecto antibacteriano individual, se ha propuesto la combinación de extractos. En este trabajo se evaluaron los efectos antibacterianos combinados en 3 extractos y sus mezclas (miel de ulmo y quillay, y polen multifloral). El contenido de polifenoles se cuantificó mediante análisis por HPLC-DAD. E. coli, S. aureus, Salmonella spp y Pseudomonas spp, L. acidophilus, B. thermosphacta se usaron como cepas patógenas de deterioro transmitidas por los alimentos en los ensayos antibacterianos. También se establecieron relaciones polifenol-bacteria. Los resultados mostraron que todos los extractos y sus combinaciones tienen actividad contra todas las cepas. Se establecieron relaciones entre todas las cepas patógenas con 4 ácidos fenólicos y 2 flavonoides, y entre 2 cepas de deterioro con 3 ácidos fenólicos y 3 flavonoides. Estos hallazgos contribuyen a la evaluación rápida de mezclas de extractos antibacterianos para usar en la industria alimentaria. ARTICLE HISTORY

Honeybee Pollen Load: Phenolic Composition and Antimicrobial Activity and Antioxidant Capacity

“…It is possible to identify the botanical origin by morphological and structural analysis of the grains under a microscope − or by using flavonoid and phenolic profiles . Research groups have outlined several potential bioactive roles for honeybee pollen including antioxidant, immunomodulatory, cardioprotective, and antimicrobial activities. ,− These activities are mainly attributed to phenolic compounds such as flavonoids. , Currently, these types of compounds are widely valued, and due to their medicinal properties, interest in bee pollen from the pharmaceutical, food, and cosmetic industries is ever increasing. , Many studies have been done in plants, − but few studies have been made on pollen loads produced by Chilean hives located in natural plant communities. , The Chilean flora has a high degree of endemism at 50%. Furthermore, the survival of some species, particularly the endemic species and the species restricted to the V Region, are threatened by indiscriminate extraction for medicinal uses.…”

“…12,13 Many studies have been done in plants, 14−16 but few studies have been made on pollen loads produced by Chilean hives located in natural plant communities. 17,18 The Chilean flora has a high degree of endemism at 50%. Furthermore, the survival of some species, particularly the endemic species and the species restricted to the V Region, are threatened by indiscriminate extraction for medicinal uses.…”

Assessment of p-cresol and phenol antifungal interactions in an arthropod defensive secretion: the case of an endemic Balkan millipede, Apfelbeckia insculpta (L. Koch, 1867) (Diplopoda: Callipodida)

“…Specifically, several molecular properties of phenolics have been linked with their antifungal potential -properties such as lipophilicity, reactivity and ability to form specific interactions between the phenolic compounds and their target receptor in cellular lipoprotein membranes [Dambolena et al, 2012;Gallucci et al, 2014;Pizolitto et al, 2015]. The last mentioned characteristic of phenolic compounds is mirrored by their ability to inactivate microbial adhesins which lead to the leakage of cytoplasmic contents [Velásquez et al, 2019].…”

Mayten Tree Seed Oil: Nutritional Value Evaluation According to Antioxidant Capacity and Bioactive Properties

“…Total polyphenolic contents (TPC) were estimated using the Folin–Ciocalteu (FC) method based on the antioxidant extract from oil, as described by Velásquez et al (2019) [ 31 ]. Gallic acid (GA) was used as the standard; therefore, the resulting values are expressed in mg GAE (100 g) −1 of seed oil.…”