Bioactive support for cell cultivation and potential grafting. Part 1: Surface modification of 2-hydroxyethyl methacrylate hydrogels for avidin immobilization

Abstract

Synthetic hydrogels are often used in biomedical applications as many of them are compatible with living tissue and moreover they can meet most criteria for artificial tissue properties. For applications in tissue engineering modification of polymer surface using some bioactive compounds (e.g. saccharides, proteins) for promoting the process of cell adhesion and proliferation is widely used. In this work, a series of modified hydrogels was prepared by three different methods: by copolymerization of 2-hydroxyethyl methacrylate with methacrylic acid, by hydrolysis and oxidation of poly(2-hydroxyethyl methacrylate) to obtain carboxyl-rich supports. The influence of the reaction conditions of the hydrogel surface treatment on the total carboxylic group content and the swelling degree was studied. The modified hydrogels were characterized by attenuated total reflectance FT-IR spectroscopy and refractive index measurements. Obtained carboxylic groups on the hydrogel surface have allowed the immobilization of avidin in two ways: 1) electrostatically through dissociated carboxylic groups and 2) covalently bonded through activated carboxylic groups by Nhydroxysuccinimide. The capacity of hydrogels for avidin immobilization was determined by Bradford spectrophotometric method. The results so far obtained from the preliminary biological tests showed that immobilized avidin on the hydrogel surface provides better adhesion and proliferation of keratinocytes compared to supports without avidin.

Design of modular gellan gum hydrogel functionalized with avidin and biotinylated adhesive ligands for cell culture applications

“…Their preliminary cell culture results rudimentarily show the attachment of keratinocytes on surface-modified avidin-pHEMA, but no 3D system or in-depth cell response analysis. [20] In contrast to the strategies mentioned above, our system aims to provide 3D soft tissue mimic for true cell encapsulation.…”

“…[18] Furthermore, this type of protein-affinity system has been exploited for many different applications in chemistry, biosciences, and tissue engineering. Indeed, modular approaches, such as the use of nanocellulose for 3D printing [19], 2-hydroxyethyl methacrylate flat substrates[20], agarose for spatial patterning [21], and porous poly-L-lactic acid scaffolds [22], have been presented and will be discussed in chapter 4.1.…”

Mechanism and technological results of sulfidation roasting of oxidized lead compounds

“…The results of previous studies [ [11], [12], [13], [14], [15], [16], [17], [18]] and the study of the characteristics of roasting products showed that sulfides of non-ferrous metals obtained by sulfidation of oxides and carbonates have high hydrophobic properties, and pyrrhotite, a product of pyrite decomposition, has a significant magnetic susceptibility. All this predetermines further methods for processing sulfidation products.…”