Bioactive substances in buffy‐coat‐derived platelet pools stored in platelet‐additive solutions

Abstract

Summary.We studied extracellular accumulation in buffycoat-derived platelet pools suspended in platelet additive solutions (PAS). 20 platelet pools were prepared from buffy coats of 60 donors and stored in either PAS I (n ¼ 10) or PAS II (n ¼ 10). Samples were drawn from the donors to determine the normal range of histamine, plasminogen activator inhibitor 1 (PAI-1), myeloperoxidase (MPO) and interleukin (IL)-6. After preparation samples were collected on days 0, 5 and 7 from the 20 platelet pools respectively, to determine extracellular accumulation of the same substances. Extracellular histamine, PAI-1 and MPO were detected in significant levels (P < 0·0001) immediately after preparation of the platelet pools. Only the extracellular level of PAI-1 increased significantly during storage from day 0 to day 7 (P < 0·01). After preparation and storage to day 7 IL-6 was found in only three of the 20 platelet pools.Even though platelets were prepared by the buffy-coat method, to produce a low leucocyte count and thereby low cytokine (IL-6) content, there was still significant contamination by other bioactive substances.

Leukoreduced blood components: Advantages and strategies for its implementation in developing countries

“…Hence during pre-storage leukoreduction, blood components can be thoroughly studied and evaluated for quality control, and various factors affecting the process of leukofiltration modified accordingly. [2122]…”

Accumulation of the neutrophil-derived protein YKL-40 during storage of various blood components

“…The mechanisms leading to development of infectious complications in relation to blood transfusion are still not known in details, but transfusion-induced impaired immune competence, donor leukocyte surface antigens and various soluble bioactive substances may play a role [2][3][4]. Presumably due to disintegration of granulocytes and platelets during storage of blood components [5,6] various granule associated bioactive substances are accumulated extracellularly in a time-dependent manner [4,7]. Some of these substances, such as myeloperoxidase (MPO), eosinophil cationic protein (ECP), eosinophil protein X (EPX), histamine, neutrophil elastase, activated complement 3 (C3a), and vascular endothelial growth factor (VEGF), are well known to play significant roles in inflammation and infectious complications [4,7,8].…”

Clinical Impact of Bioactive Substances in Blood Components: Implications for Leukocyte Filtration

“…Cytokine synthesis and subsequent accumulation appears to be reduced or even absent in platelet concentrates prepared from buffy coats and suspended in artificial media without plasma [117,118]. Presynthesized leukocyteand platelet-derived bioactive substances are, however, still accumulated during preparation and storage of these particular components [118] and may still play a detrimental role in transfusion-associated adverse effects [118].…”