Bioactive Secondary Metabolites with Unique Aromatic and Heterocyclic Structures Obtained from Terrestrial Actinomycetes Species

Abdelfattah, Mohamed S.; Arai, Midori A.; Ishibashi, Masami

doi:10.1248/cpb.c16-00038

Cited by 30 publications

(15 citation statements)

References 49 publications

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“…Actinobacteria merupakan bakteri Gram-positif yang bersifat aerob dan membentuk spora, memiliki kandungan guanin dan sitosin pada genom yang tinggi (55-57%), memiliki karakteristik koloni yang khas seperti serbuk, bertepung dan kasar, memiliki miselia bercabang sejati yang ditunjukkan pada miselium substrat dan aerial, serta mampu memproduksi pigmen (Anandan, Dharumadurai, & Manogaran, 2016;Ganesan et al, 2017;Kuncharoen, Fukasawa, Mori, Shiomi, & Tanasupawat, 2019). Actinobacteria telah dikenal sebagai bakteri penghasil senyawa bioaktif dengan beragam aktivitas biologis (Abdelfattah, Arai, & Ishibashi, 2016). Sebanyak dua pertiga dari 10.000 senyawa aktif biol ogis yang berasal dari mikroorganisme diproduksi oleh Actinobacteria (Kumari & Doss, 2016).…”

Section: Pendahuluanunclassified

“…Selain hidup bebas pada ekosistem akuatik, Actinobacteria juga dapat berasosiasi dengan inang eukariotik pada ekosistem tersebut, salah satunya adalah ikan (Abdelfattah et al, 2016;Jami, Ghanbari, Kneifel, & Domig, 2015;Lewin et al, 2017). Menurut Bernal, Campa-córdova, Saucedo, & González (2015), Actinobacteria merupakan bagian dari komunitas mikrobiota unik pada ikan.…”

Section: Pendahuluanunclassified

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Penapisan Actinobacteria Akuatik Penghasil Antibakteri Dari Ikan Bandeng (Chanos chanos) dan Belanak (Mugil cephalus) dengan Metode Double-Layer Diffusion

Kurnianto

Kusumaningrum

Lioe

2020

JPBKP

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Saluran pencernaan (terutama usus) ikan perairan estuaria merupakan salah satu ceruk lingkungan potensial Actinobacteria yang belum tereksplorasi. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi karakteristik morfologi Actinobacteria asal ikan bandeng (Chanos chanos) dan belanak (Mugil cephalus) serta mengevaluasi aktivitas antimikroba yang dihasilkannya. Penelitian ini diawali dengan mengambil usus ikan, kemudian digesta usus secara perlahan dipisahkan untuk dieksplorasi keberadaan Actinobacteria dengan menggunakan media isolasi selektif. Isolat yang diperoleh dikarakterisasi berdasarkan ciri makroskopik dan mikroskopik, serta dilakukan penapisan antibakteri awal menggunakan metode double-layer diffusion. Isolat dengan zona penghambatan terbaik dipilih untuk dilakukan produksi dan ekstraksi senyawa antibakteri, serta uji aktivitas antibakteri dengan metode difusi cakram terhadap bakteri uji Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa dan Escherichia coli. Sebanyak 44 isolat Actinobacteria telah diisolasi dari digesta usus ikan bandeng (Chanos chanos) dan belanak (Mugil cephalus) menggunakan media strach casein dan actinomycete isolation agar. Sebagian besar isolat yang diperoleh menunjukkan karakteristik morfologi genus Streptomyces sp., seperti koloni memiliki tekstur menyerupai serbuk, bertepung dan kasar, memiliki aerial miselium berwarna putih dan substrat miselium berwarna krim susu, serta memiliki bentuk rantai spora rectus-flexibilis. Proses penapisan antibakteri isolat Actinobacteria menunjukkan 22 isolat memiliki indeks penghambatan terhadap sedikitnya satu bakteri uji, dengan aktivitas terbaik ditunjukkan oleh isolat A-SCA-11. Uji antibakteri terhadap ekstrak kasar isolat A-SCA-11 menunjukkan aktivitas antibakteri berspektrum luas yang mampu menghambat seluruh bakteri uji dengan zona hambat tertinggi pada P. aeruginosa. AbstractThe gut of estuary fish is one of the potential novel niches of Actinobacteria that has not yet been explored. This study aimed to isolate and identify the morphological characteristics of Actinobacteria from milkfish (Chanos chanos) and blue-spot mullet fish (Mugil cephalus) and to evaluate the antibacterial activity produced. This research was started by taking the fish gut, and then the digesta were slowly separated to explore the presence of Actinobacteria using selective isolation media. The isolates obtained were characterized by macroscopic and microscopic characteristics, and antibacterial preliminary screening of isolates was performed using a double-layer diffusion method. The isolates with the best inhibition zone were selected for production and extraction of antibacterial compounds, and antibacterial activity tests using the disk-diffusion method against the test bacteria Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, and Escherichia coli. A total of 44 isolates of Actinobacteria have been isolated from the gut of fish using starch casein and actinomycete isolation agar. Most isolates showed morphological characteristics of the genus Streptomyces sp., such as colonies with a tough or powdery texture, antibacterial have white aerial mycelium and milk-cream substrate mycelium, and rectus-flexibilis spore chain. The antibacterial preliminary screening of Actinobacteria isolates showed 22 isolates had inhibitory index against at least one test bacterium, with the best activity indicated by A-SCA-11. Antibacterial test of A-SCA-11 crude extract showed broad-spectrum antibacterial activity that was able to inhibit all test bacteria with the highest inhibitory zone on P. aeruginosa.

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Section: Pendahuluanunclassified

Penapisan Actinobacteria Akuatik Penghasil Antibakteri Dari Ikan Bandeng (Chanos chanos) dan Belanak (Mugil cephalus) dengan Metode Double-Layer Diffusion

Kurnianto

Kusumaningrum

Lioe

2020

JPBKP

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“…We herein describe the isolation, structural elucidation and evaluation of the TRAIL resistance-overcoming activities of compounds 1-6 in AGS cell lines because we are interested in targeting the TRAIL-related cancer-selective apoptosis-inducing pathway using microbial natural compounds. 15 RESULTS AND DISCUSSION Streptomyces sp. IFM 11490 was isolated from a soil sample collected from Sapporo in Hokkaido, Japan.…”

Section: Introductionmentioning

confidence: 99%

Elmenols C-H, new angucycline derivatives isolated from a culture of Streptomyces sp. IFM 11490

et al. 2017

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“…Actinomycetes are comprised of diverse group of organisms that are Gram positive with guanine plus cytosine content in their DNA being greater than 55M%. 1 The term actinomycetes are coined from two greek words, Atkis meaning ray and Mykesmeaning fungus. 2 This is because the organisms have both fungi and bacteria like features.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2017

IJMBOA

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The current study was carried out to isolate actinomycetes from the geothermal vents of Menengai crater. Soil samples were collected from vents A and D in sterile polythene papers and transported to Egerton University, Department of biological sciences laboratories. The samples were air dried on the benches for one week. To kill vegetative bacteria, the soil samples were heat in a hot air oven for 1h before serial dilution to 10-6. The samples separately plated on Starch casein agar, Luria Bertani agar and starch nitrate agar in which nystatin and nalidixic acid had been added to reduce the growth of fungi and other types of bacteria. Incubation was carried out at 30 o C for up to a period of one Month. The isolated actinomycetes were characterized by cultural, morphological and biochemical means. There was no significant difference in the number of actinomycetes isolated between vents A and vents D(P=0.439). However, the number of actinomycetes isolated using the three isolation media varied significantly (F=37, P=0.03). Totally, 16 actinomycetes were isolated from the vents. It is recommended that the isolates be tested for antagonism against pathogenic microorganism.

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Bioactive Secondary Metabolites with Unique Aromatic and Heterocyclic Structures Obtained from Terrestrial Actinomycetes Species

Cited by 30 publications

References 49 publications

Penapisan Actinobacteria Akuatik Penghasil Antibakteri Dari Ikan Bandeng (Chanos chanos) dan Belanak (Mugil cephalus) dengan Metode Double-Layer Diffusion

Penapisan Actinobacteria Akuatik Penghasil Antibakteri Dari Ikan Bandeng (Chanos chanos) dan Belanak (Mugil cephalus) dengan Metode Double-Layer Diffusion

Elmenols C-H, new angucycline derivatives isolated from a culture of Streptomyces sp. IFM 11490

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