Bioactive Secondary Metabolite from Endophytic Aspergillus Tubenginses ASH4 Isolated from Hyoscyamus muticus: Antimicrobial, Antibiofilm, Antioxidant and Anticancer Activity

Elkhouly, Heba I.; Hamed, Ahmed A.; Hosainy, Asmaa M El; Ghareeb, Mosad A.; Sidkey, Nagwa M.

doi:10.5530/pj.2021.13.55

Cited by 28 publications

(20 citation statements)

References 28 publications

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“…The extensive and uncontrolled utilization of medicinal plants may add to the ecological burden in terms of overutilization of endangered and rare species and in disturbing the ecological balance [ 9 ]. In this context, endophytes associated with medicinal plants present an eco-friendly alternative source of bioactive metabolites, given that endophytes may cross talk with the host medicinal plants in terms of their biosynthetic routes or that they may be the original producers of some active ingredients, or at least may provide the host organisms with extra chemical defense to cope with the surrounding stress conditions [ 10 , 11 , 12 , 13 ]. The abundance of endophytic fungi within the host medicinal plants may be associated with the pharmacological actions linked to the plant part used [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Chemical and Biological Review of Endophytic Fungi Associated with Morus sp. (Moraceae) and In Silico Study of Their Antidiabetic Potential

et al. 2023

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The chronic nature of diabetes mellitus motivates the quest for novel agents to improve its management. The scarcity and prior uncontrolled utilization of medicinal plants have encouraged researchers to seek new sources of promising compounds. Recently, endophytes have presented as eco-friendly leading sources for bioactive metabolites. This article reviewed the endophytic fungi associated with Morus species and their isolated compounds, in addition to the biological activities tested on their extracts and chemical constituents. The relevant literature was collected from the years 2008–2022 from PubMed and Web of Science databases. Notably, no antidiabetic activity was reported for any of the Morus-associated endophytic fungal extracts or their twenty-one previously isolated compounds. This encouraged us to perform an in silico study on the previously isolated compounds to explore their possible antidiabetic potential. Furthermore, pharmacokinetic and dynamic stability studies were performed on these compounds. Upon molecular docking, Colletotrichalactone A (14) showed a promising antidiabetic activity due to the inhibition of the α-amylase local target and the human sodium-glucose cotransporter 2 (hSGT2) systemic target with safe pharmacokinetic features. These results provide an in silico interpretation of the possible anti-diabetic potential of Morus endophytic metabolites, yet further study is required.

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Section: Introductionmentioning

confidence: 99%

Chemical and Biological Review of Endophytic Fungi Associated with Morus sp. (Moraceae) and In Silico Study of Their Antidiabetic Potential

et al. 2023

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“…Moreover, the bacterial biofilm formation has been found to play a critical role in the persistence of bacterial nosocomial infections. This phenomenon facilitates bacterial colonization on living or non-living surfaces and is associated with 65-80% of all clinical infections [4][5][6]. Due to such adaptive changes; biofilm-forming bacteria are 10-to 1000-fold more resistant to conventional antibiotics, which thus present a great challenge to develop antimicrobials specifically to treat biofilms [7].…”

Section: Introductionmentioning

confidence: 99%

LC-MS/MS profiling, antibiofilm, antimicrobial and bacterial growth kinetic studies of Pluchea dioscoridis extracts

El-Shazly

Hamed

Kabary

et al. 2022

AChrom

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The therapeutical applications of ornamental plants have been categorized to be of a great effectiveness in multiple industries from ancient times until present days. Pluchea dioscoridis is widely known Egyptian wooden plant that has been extensively applied for different medicinal purposes. In this study, LC-ESI-MS/MS analysis of the potent antimicrobial ethyl acetate and n-butanol extracts of P. dioscoridis leaves led to identification of 28 and 21 compounds, respectively. The identified compounds were categorized as phenolic acids, phenolic acids derivatives, organic acids, flavonoids (aglycones and glycosides), secoiridoids, coumarin derivatives, and gallotannins derivatives. Among them, caffeic acid 3-sulfate was the most predominate in the investigated extracts followed by ferulic acid and dicaffeoyl-quinic acid. Also, the antimicrobial potentiality of different extracts was evaluated against different pathogenic microbes including Enterobacter cloacae, Micrococcus leutus, Aeromonas hydrophila, Bacillus subtilis, Bacillus cereus, Bacillus lichneformis and Clostridium species. Furthermore, different concentrations of the most potent extract were assayed for antibacterial efficacy on growth curve kinetics against the susceptible bacteria along 4days incubation period. Our gathered data confirmed that, the antimicrobial activity against tested bacteria was different according to the solvent used in the extraction process. Mostly, all the extracts showed a wide spectrum antibacterial activity except the plant water extract which shows a mild activity against Clostridium sp. only. Based on the highest inhibition zone diameter, the ethyl acetate extract followed by butanol extract exhibited the highest inhibition zone with Micrococcus luteus and B. subtilis (20.0 and 18.5 mm) respectively. Determining the effect of ethyl acetate extract at different concentration (0, 0.66, 1.66, 3.33, 6.67, 13.34 and 20.01 mg mL−1) on M. luteus growth kinetics, the data assured that the antibacterial activity shows concentration dependent manner with the highest antibacterial activity at 20.01 mg mL−1 culture. The data also confirmed that, none of the selected concentration showed bactericidal activity in the prepared cultures, and with the prolonged incubation period the bacteria acquire resistance against the extract beginning from second or third day of incubation.

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“…Almost 80% of the world’s antibiotics are known to be derived from Actinomycetes, primarily the species Streptomyces and Micromonospora (van der Meij et al 2017 ). Most antibiotics are no longer effective against the majority of bacteria-caused infections (Ghareeb et al 2015 ; Elkhouly et al 2021a ). Staphylococcus aureus is a well-known organism that causes illnesses such as pneumonia and recently has gained resistance to the majority of antibiotic groups (Tong et al 2015 ; Hamed et al 2020a , b ; El-Shazly et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Bioactive secondary metabolites from marine Actinomyces sp. AW6 with an evaluation of ADME-related physicochemical properties

et al. 2022

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This study was designed to evaluate the antimicrobial, antioxidant, and cytotoxic potentials of the marine actinomycetes spp. isolated from the Red Sea water, Hurghada, Egypt. Out of 80 actinomycetes isolates, one isolate AW6 was selected based on its antioxidant activity (IC50 about 5.24 µg/mL which scavenged 91% of formed DPPH free radicals) and antimicrobial potential against E. coli, S. aureus, B. subtilis, and P. aeruginosa, A. niger, and C. albicans. The strain was identified based on phenotypic and genotypic analysis, and deposited in the GenBank with accession number OK090864.1. Cultivation of the selected strain on rice, chromatographic purification, and structural elucidation led to the isolation of two compounds C1: umbelliferone, and C2: 1-methoxy-3-methyl-8-hydroxy-anthraquinone. The antimicrobial activity of the obtained compounds showed that C1 and C2 have low antibacterial activity toward S. aureus and E. coli with no pronounced activity toward P. aeruginosa, C. albicans, and A. niger. Additionally, the antioxidant activity of C1 and C2 revealed that C2 has a good antioxidant activity, with DPPH scavenging activity reaching (55.25%), followed by C1 (30.20%). Moreover, both compounds displayed anti-Gyr-B enzyme activity with IC50 value of (3.79 ± 0.21 µM) for C1, and (IC50 = 13 ± 0.71 µM) for C2. The ADME-related physicochemical properties of the obtained compound were predicted using SwissADME web tools and the ProToxii webserver was used to estimate in silico toxicity.

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Bioactive Secondary Metabolite from Endophytic Aspergillus Tubenginses ASH4 Isolated from Hyoscyamus muticus: Antimicrobial, Antibiofilm, Antioxidant and Anticancer Activity

Cited by 28 publications

References 28 publications

Chemical and Biological Review of Endophytic Fungi Associated with Morus sp. (Moraceae) and In Silico Study of Their Antidiabetic Potential

Chemical and Biological Review of Endophytic Fungi Associated with Morus sp. (Moraceae) and In Silico Study of Their Antidiabetic Potential

LC-MS/MS profiling, antibiofilm, antimicrobial and bacterial growth kinetic studies of Pluchea dioscoridis extracts

Bioactive secondary metabolites from marine Actinomyces sp. AW6 with an evaluation of ADME-related physicochemical properties

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