Bioactive proteins from mushrooms

Xu, Xiaofei; Yan, Huidan; Chen, Jian; Zhang, Xuewu

doi:10.1016/j.biotechadv.2011.05.003

Cited by 239 publications

(143 citation statements)

References 71 publications

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“…Finally, fungal lectins have been considered widely as diagnostic tools based on their highly specific binding of glycoconjugates and on the altered glycosylation profiles observed in various diseases. These potential medical applications of fungal defense lectins and protease inhibitors have been described in more detail in recent reviews (Erjavec et al 2012;Hassan et al 2015;Xu et al 2011). …”

Section: Potential Applicationsmentioning

confidence: 99%

“…Many reviews on mushroom lectins and protease inhibitors have been published recently but mainly viewing their potential application in human medicine (Dunaevsky et al 2014;Erjavec et al 2012;Hassan et al 2015;Kobayashi and Kawagishi 2014;Wong et al 2010;Xu et al 2011). The present review focuses on fruiting body lectins and protease inhibitors that have been characterized at the genetic, molecular, structural, and functional levels and that exhibit toxicity to nematodes and/or insects (Table 1).…”

Section: Introductionmentioning

confidence: 99%

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Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies

Sabotič

Ohm

Künzler

2015

Appl Microbiol Biotechnol

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Fruiting bodies or sporocarps of dikaryotic (ascomycetous and basidiomycetous) fungi, commonly referred to as mushrooms, are often rich in entomotoxic and nematotoxic proteins that include lectins and protease inhibitors. These protein toxins are thought to act as effectors of an innate defense system of mushrooms against animal predators including fungivorous insects and nematodes. In this review, we summarize current knowledge about the structures, target molecules, and regulation of the biosynthesis of the best characterized representatives of these fungal defense proteins, including galectins, beta-trefoil-type lectins, actinoporin-type lectins, beta-propeller-type lectins and beta-trefoil-type chimerolectins, as well as mycospin and mycocypin families of protease inhibitors. We also present an overview of the phylogenetic distribution of these proteins among a selection of fungal genomes and draw some conclusions about their evolution and physiological function. Finally, we present an outlook for future research directions in this field and their potential applications in medicine and crop protection.

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Section: Potential Applicationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies

Sabotič

Ohm

Künzler

2015

Appl Microbiol Biotechnol

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“…They may be edible or non-edible mushroom species. [13][14][15] Trametes ochracea is a white coloured wood rotting fungi, used to produce laccase enzyme. 16 The present investigation was aimed to identify phytochemicals in different solvent extracts and this extract was evaluated for different in vitro biological activities (antioxidant, anti-inflammatory, cytotoxicity).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical analysis and in vitro antioxidant, antimicrobial, anti-inflammatory and cytotoxicity activities of wood rotting fungi, Trametes ochracea

Govindappa¹,

Roshan²,

Nithi³

et al. 2015

Phcog J

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Objective: The present research was aimed to identify the metabolites in the methaolic and hexane extract of Trametes ochracea and evaluated these extracts to know their in vitro biological activities. Materials and methods: Two solvent extracts of T. ochracea was subjected to phytochemical analysis. The two solvent extracts were subjected to in vitro biological activity viz., antioxidant, anti-inflammatory and cytoxicity assays. Results: The methanol extract yielded important phytochemicals viz., saponins, flavonoids, alkaloids, steroids, phenols and tannins compared to hexane. The methanolic extract has shown strong antioxidant activity in all tested methods. The methanol extract was effectively inhibited the heat induced hemolysis, antilipoxygenase activity and also stabilized the membrane, avoided the membrane denaturation, proteinase and xanthine oxidase inhibition.The onion root meristametic cells were inhibited due to toxicity of methanol extract by possessing various cellular abnormalities in various stages of actively growing cells. The yeast cells were dead due to toxicity of methanol extract by possessing cell necrosis and also fragmented the cell DNA. Conclusion: The obtained results clearly indicates that Trametes ochracea methanol extract is having potent phytochemicals, which plays important role in antioxidant, anti-inflammatory, cytotoxicity assays. The further research is needed to identify the exact mechanism is by action of one or combination of active phytochemicals.

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“…Addition to low molecular weight (MW) secondary metabolites, mushrooms produce a variety of proteins, such as lectins, with antitumor, antiviral, antimicrobial, antioxidative, and/or immunomodulatory activities (31). Recent genomic and computational studies have further identified an enormous number of genes encoding small proteins from fungal genomes (32).…”

mentioning

confidence: 99%

“…Recent genomic and computational studies have further identified an enormous number of genes encoding small proteins from fungal genomes (32). These small proteins awaiting assigned functions can become useful biotechnological and biomedical agents (31).…”

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confidence: 99%

Cytotoxic protein from the mushroom Coprinus comatus possesses a unique mode for glycan binding and specificity

Zhang

Yang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Glycans possess significant chemical diversity; glycan binding proteins (GBPs) recognize specific glycans to translate their structures to functions in various physiological and pathological processes. Therefore, the discovery and characterization of novel GBPs and characterization of glycan-GBP interactions are significant to provide potential targets for therapeutic intervention of many diseases. Here, we report the biochemical, functional, and structural characterization of a 130-amino-acid protein, Y3, from the mushroom Coprinus comatus. Biochemical studies of recombinant Y3 from a yeast expression system demonstrated the protein is a unique GBP. Additionally, we show that Y3 exhibits selective and potent cytotoxicity toward human T-cell leukemia Jurkat cells compared with a panel of cancer cell lines via inducing caspase-dependent apoptosis. Screening of a glycan array demonstrated GalNAcβ1-4(Fucα1-3)GlcNAc (LDNF) as a specific Y3-binding ligand. To provide a structural basis for function, the crystal structure was solved to a resolution of 1.2 Å, revealing a single-domain αβα-sandwich motif. Two monomers were dimerized to form a large 10-stranded, antiparallel β-sheet flanked by α-helices on each side, representing a unique oligomerization mode among GBPs. A large glycan binding pocket extends into the dimeric interface, and docking of LDNF identified key residues for glycan interactions. Disruption of residues predicted to be involved in LDNF/Y3 interactions resulted in the significant loss of binding to Jurkat T-cells and severely impaired their cytotoxicity. Collectively, these results demonstrate Y3 to be a GBP with selective cytotoxicity toward human T-cell leukemia cells and indicate its potential use in cancer diagnosis and treatment.

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Bioactive proteins from mushrooms

Cited by 239 publications

References 71 publications

Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies

Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies

Phytochemical analysis and in vitro antioxidant, antimicrobial, anti-inflammatory and cytotoxicity activities of wood rotting fungi, Trametes ochracea

Cytotoxic protein from the mushroom Coprinus comatus possesses a unique mode for glycan binding and specificity

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