Bioactive potential of Streptomyces associated with marine sponges

Dharmaraj, Selvakumar; Sumantha, Alagarsamy

doi:10.1007/s11274-009-0096-1

Cited by 30 publications

(15 citation statements)

References 34 publications

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“…The molecular weight of the purified enzyme was found to be 97.4 kDa. This finding was in agreement with the previous observation, in which bacterial LA existed with molecular mass in the range of 85–140 kDa (Dharmaraj and Dhevendaran 2010; Dharmaraj and Sumantha 2009). However, these results were different from the reports of LA from bacterial species having higher molecular weight range of 140–160 kDa (Kumar et al 2011; Aghaiypour et al 2001).…”

Section: Resultssupporting

confidence: 94%

Mitigation of acrylamide by l-asparaginase from Bacillus subtilis KDPS1 and analysis of degradation products by HPLC and HPTLC

et al. 2016

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The use of bacterial l-asparaginase (LA) is one of the alternative approaches for acrylamide reduction in food stuffs as it catalyzes the conversion of l-asparagine to l-aspartic acid and ammonia. In present investigation, purification of extracellular LA from isolate of Bacillus subtilis sp. strain KDPS-1 was carried out by solid state fermentation process. The effects of solid substrates, initial moisture content, moistening agents, temperature, and incubation time on LA production was studied, and the highest asparaginase activity (47 IU/ml) was achieved in the medium having orange peel as substrate. The enzyme was purified to homogeneity by diethylaminoethyl (DEAE) cellulose ion exchange chromatography; with 84.89 % yield and 12.11 fold purity. LA showed stimulant activity against β-mercaptoethanol and was greatly inhibited by Zn2+ and Hg2+ metal ions. Reduction of acrylamide in fried potatoes was detected by high performance liquid chromatography, which showed clear degradation of acrylamide by height and area (%) in the chromatograms of standard sample to that of the test sample. Hydrolysates analysis by high performance thin layer chromatography confirmed the test sample to be LA.

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Section: Resultssupporting

confidence: 94%

Mitigation of acrylamide by l-asparaginase from Bacillus subtilis KDPS1 and analysis of degradation products by HPLC and HPTLC

et al. 2016

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“…However, the authors did not discuss any aspect related to the importance of media used for the isolation or growth of the strains obtained, or even related to the activity of the extracts obtained. A single growth medium was used by Dharmaraj & Sumantha (2009) for the isolation of 94 streptomycetes strains from four species of marine sponges. More than 28% of the strains isolated produced extracts that displayed broad antibacterial activities.…”

Section: Resultsmentioning

confidence: 99%

Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites

Kossuga

Romminger

Xavier

et al. 2012

Rev. bras. farmacogn.

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Abstract:In the present investigation we evaluate methods for the isolation and growth of marine-derived fungal strains in artificial media for the production of secondary metabolites. Inoculation of marine macroorganisms fragments in Petri dishes proved to be the most convenient procedure for the isolation of the largest number of strains. Among the growth media used, 3% malt extract showed the best result for strains isolation and growth, and yielded the largest number of strains from marine macroorganisms. The percentage of strains isolated using each of the growth media which yielded cytotoxic and/or antibiotic extracts was in the range of 23-35%, regardless of the growth media used. Further investigation of extracts obtained from different marine-derived fungal strains yielded several bioactive secondary metabolites, among which (E)-4-methoxy-5-(3-methoxybut-1-enyl)-6-methyl-2H-pyran-2-one is a new metabolite isolated from the Penicillium paxilli strain Ma(G)K.

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“…Recently about 94 isolates of Streptomyces were found to be associated with four marine sponges namely Callyspongia diffusa, Mycale mytilorum, Tedania anhelans and Dysidea fragilis collected from South West coast of India. These strains showed antibacterial and antifungal activities (Dharmaraj and Sumantha 2009).…”

Section: Metabolite Production By Marine Streptomycesmentioning

confidence: 99%

Marine Streptomyces as a novel source of bioactive substances

Dharmaraj

2010

World J Microbiol Biotechnol

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160

102

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Marine actinobacteria are the most economically as well as biotechnologically valuable prokaryotes. Representative genera of marine actinobacteria include Actinomadura, and several others. Among the genera of marine actinobacteria, the genus Streptomyces is represented in nature by the largest number of species and varieties, which differ greatly in their morphology, physiology, and biochemical activities. Marine Streptomyces occur in different biological sources such as fishes, molluscs, sponges, seaweeds and mangroves, besides seawater and sediments. In this review an evaluation is made on the present state of research on marine Streptomyces and its perspectives. The highlights include the production of metabolites such as antibiotics, anticancer compounds, enzymes, enzyme inhibitors and pigments by marine Streptomyces and their application as single cell protein and as probiotics in aquaculture. The marine environment contains a wide range of distinct Streptomyces that are not present in the terrestrial environment. With increasing advancement in science and technology, there would be greater demands in future for new bioactive compounds synthesised by Streptomyces from various marine sources.

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Bioactive potential of Streptomyces associated with marine sponges

Cited by 30 publications

References 34 publications

Mitigation of acrylamide by l-asparaginase from Bacillus subtilis KDPS1 and analysis of degradation products by HPLC and HPTLC

Mitigation of acrylamide by l-asparaginase from Bacillus subtilis KDPS1 and analysis of degradation products by HPLC and HPTLC

Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites

Marine Streptomyces as a novel source of bioactive substances

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