Bioactive potential of actinobacteria isolated from certain under-studied regions in India

Abstract

The present study reports the bioactive potential of actinobacteria isolated from certain under-studied regions in India. Soil and sediment samples and mangrove leaves were collected from 16 different under-studied regions in India. Actinobacteria was isolated by adopting selective isolation methods. Totally 158 actinobaterial cultures were selected from the collected terrestrial, marine and plant samples. More number of colonies was isolated from magnesite area, Kolli Hills and forest area in Himachal Pradesh. Majority of the isolates produced powdery (40%) and leathery (25%) colonies with white (38%) or Gray (37%) colour aerial mycelium. Bioactive compound from all the isolates were produced by agar surface fermentation and its activity was tested by agar plug method against S. aureus, E. coli and C. albicans. About 64 out of 158 cultures showed antibacterial activity in which 62 cultures was active against S. aureus whereas 26 were active against E. coli. Twenty three actinobacterial cultures were exhibited antifungal activity. About 11 actinobacterial cultures were active against both S. aureus and E. coli. In antifungal testing whereas fourteen actinobacterial cultures were found to be active against S. aureus, E. coli and C. albicans. Maximum of 21 antibacterial cultures and antifungal cultures were obtained from Magnesite soil followed by 12 antibacterial and 7 antifungal cultures were obtained from the soil sample collected from Himachal Pradesh. This evidenced that the under-studied ecosystems in India are the promising source for bioactive actinobacteria with broad spectrum antibacterial and antifungal activity. Further studies on the potential actinobacterial strains result in the isolation of broad spectrum antimicrobial metabolites.

Broad Spectrum Antifungal Activity and Anticancer Potential of Secondary Metabolites from Cave Soil Streptomyces sp. strain YC69

“…In the present study, more isolates displayed good growth in the SCA medium compared to other culture media used, and the soil pre-treatment reduced contamination. Many studies on soil actinomycetes isolation reported Streptomyces as a signi cant population and cross streak method for the primary screening of isolates as an effective technique to determine potent organisms (Radhakrishnan et al 2016;Riquelme et al 2017).…”

Isolation, characterization and identification of antibiofouling metabolite from mangrove derived Streptomyces sampsonii PM33

“…Indian marine ecosystems are the promising source for bioactive actinobacteria with diverse biological activities 12,13 . Inspite of recent research on the marine actinobacterial antifouling metabolites showing the novel and precise mechanisms, the chemical structure of the compound, field assays and toxicity are yet to be defined 14,15 .…”