Bioactive Polyphenols from the Methanol Extract ofCnicus arvensis(L.) Roth Demonstrated Antinociceptive and Central Nervous System Depressant Activities in Mice

Abstract

Cnicus arvensis is used by many ethnic groups for inflammation, pain, and other ailments. In this study, reducing sugar, carbohydrate, alkaloid, steroid, tannin, flavonoid, and saponin groups were identified using standard chromogenic method. In high-performance liquid chromatography, vanillic acid and epicatechin were identified in the extract. Antinociceptive test by acetic acid induced writhing inhibition resulted 43.17 and 95.08% inhibition for 100 and 200 mg/kg body weight, comparing with standard diclofenac Na with 74.86% inhibition for 25 mg/kg body weight. In formalin induced paw licking test for antinociceptive activity, the extract inhibited 69.87 and 75.55% licking for 150 and 300 mg/kg body weight comparing with the inhibition (68.56%) of diclofenac Na for 10 mg/kg body weight at first phase. At late phase, the extract showed 73.12 and 87.46% licking comparing with licking inhibition (71.69%) by diclofenac Na at the same dose. In open field test for CNS depressant activity, the extract showed depression of locomotor activity for 150 and 300 mg/kg body weight comparing with diazepam for 10 mg/kg body weight. All results were statistically significant (P < 0.01). The identified polyphenols are reputed for antinociceptive and CNS depressant activity. The present findings support the use of this plant in pain.

Antioxidant, Antinociceptive and CNS Activities of Viscum orientale and High Sensitive Quantification of Bioactive Polyphenols by UPLC

“…The obtained filtrate was concentrated at low temperature (4°C) and reduced pressure using a rotary vacuum evaporator (R-210, Buchi, Switzerland) and dried. The gummy concentrate of 10 g (4.35% yield) was obtained and designated as crude methanol extract of V. orientale leaves (MEVOL) ( Rahman et al, 2015b ). The extract was then freeze-dried with Savant Refrigerated Vapor Trap and preserved in refrigerator for further work in future.…”

Green tea polyphenols and their potential role in health and disease

“…Polyphenols have also been implicated in many other chronic conditions such as Parkinson disease, Alzheimer's disease, antinociceptive, immune response, malignancy, anticalcification, hypertension, health and development, etc. Srinivasan and Lahiri 2015;Rahman et al 2015;Magrone and Jirillo 2015;Signorelli et al 2015;Wang et al 2015;Teles et al 2015;Ly et al 2015). There is a plethora of information that reflects on the fact that activities of green tea polyphenolic compounds can control chronic diseases that are mediated through persistent inflammation.…”

Reveals of New Candidate Active Components in Hemerocallis Radix and Its Anti-Depression Action of Mechanism Based on Network Pharmacology Approach

“…Vanillic acid is a phenolic acid with formula C 8 H 8 O 4 , found in some forms of vanilla and many other plant extracts. It is reported to possess strong antioxidant, anti-inflammatory, antinociceptive, and neuroprotective effects [37][38][39][40]. It has also been proved that vanillic acid has hepatoprotective effect, and is often used in the treatment of depression in TCM, and can suppress hepatic fibrosis in chronic liver injury [41].…”