Bioactive polyphenol profiling and in-vitro antioxidant activity of Tinospora cordifolia Miers ex Hook F and Thoms : A potential ingredient for functional food development

Khan, Tabassum; Ipshita, AH; Mazumdar, Rajib Kumar; Abdullah, Abu Tareq Mohammad; Islam, Gmr; Rahman, MM

doi:10.3329/bjsir.v55i1.46729

Cited by 17 publications

(10 citation statements)

References 11 publications

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“…In addition, ImmuPlus ® is composed of the following plant parts: Tinospora cordifolia , Ocimum sanctum , Whitania somnifera , Andrographis paniculata, and Azadirachta indica . T. cordifolia contains 20.4% tannins and 2.19% flavonoids for antioxidant activity [ 59 ]; O. sanctum contains essential oils (24.15% camphor, 23.67% eugenol, and 13.47% eucalyptol) with antimicrobial properties [ 7 ]; W. somnifera contains flavonoids, tannins, and alkaloids with antimicrobial and antifungal properties [ 60 ]; A. paniculata contains 8.06% flavonoids and 10.22% essential oils with immunomodulatory and anti-inflammatory properties [ 8 , 61 ]; A. indica contains tannins, flavonoids, and essential oils with anti-inflammatory and antioxidant activity [ 62 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of a Polyherbal Dietary Additive on Performance, Dietary Energetics, Carcass Traits, and Blood Metabolites of Finishing Lambs

et al. 2022

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The objective of this study was to evaluate the effects of dietary supplementation of a polyherbal additive (PA) containing hydrolyzable tannins, flavonoids, and essential oils on productive performance, dietary energetics, carcass and meat characteristics, and blood metabolites of lambs in their finishing phase. Twenty-eight Pelibuey × Katahdin lambs (20.52 ± 0.88 kg body weight (BW)) were housed in individual pens and assigned to four treatments (n = 7) with different doses of PA: 0 (CON), 1 (PA1), 2 (PA2), and 3 (PA3) g of PA kg−1 of DM for 56 days. Compared to the CON, lambs in PA1 treatment had higher average daily gain (p = 0.03), higher dietary energy utilization (p = 0.01), greater backfat thickness (p = 0.02), greater Longissimus dorsi muscle area (p = 0.01), and better feed conversion ratio (p = 0.02). PA supplementation did not affect (p > 0.05) dry matter intake, carcass yield, biometric measures, and meat chemical composition. All hematological and most of the blood biochemical parameters were similar in lambs of all treatments (p > 0.05). However, compared to the CON, lambs assigned to the PA3 treatment had lower serum urea concentration (p = 0.05) and higher serum albumin concentration (p = 0.03). In conclusion, low doses of PA could be used as a growth promoter in finishing lambs without affecting dry matter intake, carcass yield, meat chemical composition, and health status of the lambs. However, more in vivo research is needed to better understand the impact of bioactive compounds from PA used on productivity, metabolism, and health status of finishing lambs.

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Section: Methodsmentioning

confidence: 99%

Effects of a Polyherbal Dietary Additive on Performance, Dietary Energetics, Carcass Traits, and Blood Metabolites of Finishing Lambs

et al. 2022

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“…The wide-spectrum therapeutic activities of T. cordiofolia are due to the presence of numerous key bioactive compounds. T. cordiofolia was shown to be responsible for five phyto-constituents out of ten in the current investigation, these are gallic acid, magnoflorine, rutin, β-ecdysone and rosmarinic acid [ 22 – 24 ]. Aloe barbadensis or Aloe vera, has been classically used for skin disorders.…”

Section: Discussionmentioning

confidence: 99%

Identification, Validation and Standardization of Bioactive Molecules Using UPLC/MS-QToF, UHPLC and HPTLC in Divya-Denguenil-Vati: A Penta-Herbal Formulation for Dengue Fever

et al. 2022

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For the last fifty years, Dengue has been one of the most common mosquito-borne arboviral infections which has spread over the tropical and subtropical world. Divya-Denguenil-Vati (DNV) has been formulated by blending five specific herbs for effective resolution of Dengue fever. In the present study, we aimed to identify, develop, validate, and standardize methods for Divya-Denguenil-Vati (DNV), on UHPLC and HPTLC analytical platforms, with rapid, sensitive, accurate and rugged attributes. At first, 97 phyto-constituents were identified by UPLC/MS-QToF in Divya-Denguenil-Vati. UHPLC method was then developed and validated for simultaneous determination of gallic acid, 5-HMF, protocatechuic acid, magnoflorine, methyl gallate, berberine, rutin, ellagic acid, β-ecdysone and rosmarinic acid in DNV. Four selected markers, gallic acid, rosmarinic acid, magnoflorine and rutin were further developed and validated on HPTLC. Analytical processes were validated as per ICH Q2 (R1) guidelines; and were found linear (r 2 > 0.99), sensitive, precise (%RSD < 5%), and accurate, as indicated by high recovery values (88-105%). The limit of detection and quantification were also established for these phytometabolites, with their respective RSDs within 5% limits. Finally, these validated methods were employed to test twenty six different commercial batches of DNV. The quality, reproducibility and consistency of DNV have been well established using these developed and reliable analytical tools. These analytical strategies successfully set a path forward for robust quality evaluation and standardization of Divya-Denguenil-Vati, and other related herbal formulations.

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“…Furthermore, some other important polyphenols were also present in the extracts. These bioactive mixes are acknowledged for their oxide reduction properties (Diep et al, 2020;Khan et al, 2020;Št'astná et al, 2019), which might assume a momentous role in the mopping up of ROS and resultant antioxidant activity. The phenolic hydroxyl groups of polyphenol compounds act as hydrogen donors to directly react with radicals and reduce the activities of…”

Section: Discussionmentioning

confidence: 99%

Profiling of bioactive compounds and antioxidant activity of aquatic weedIpomoea aquatica

Roy

Zilani²,

Alrashada

et al. 2022

Aquaculture Fish & Fisheries

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Water spinach, Ipomoea aquatica, is a common semi‐aquatic weed that is used as an ingredient in fish feed to support its growth and defence system. The present study aimed to identify and quantify bioactive polyphenols through HPLC‐DAD (High Performance Liquid Chromatograpgy ‐ Diode Array Detector) analysis and to evaluate the antioxidant activity of the aquatic weed I. aquatica available in Bangladesh. Different bioactive phytochemical groups were qualitatively appraised in crude ethanol extract, aqueous and n‐hexane fractions of water spinach. The total polyphenol and flavonoids content were spectrophotometrically discerned using Folin–Chiocalteu and aluminium chloride, respectively. Bioactive compounds of the extracts were identified by the HPLC‐DAD method. Furthermore, 2,2‐diphenyl‐1‐picryldydrazyl (DPPH) and hydroxyl‐radical scavenging and reducing power assays were followed to assess antioxidant activity. The experimental extracts confirmed the presence of different bioactive phytochemical groups. The crude extract contained the highest content of both polyphenol (94.93 mg gallic acid (GA) equivalent per gram dry extract) and flavonoids (373.30 mg quercetin equivalent per gram dry extract). Besides, in HPLC (High Performance Liquid Chromatogrphy) analysis, ellagic acid was present at the highest concentration both in crude (337.26 mg/100 g dry extract) and aqueous (280.06 mg/100 g dry extract) extracts. However, the n‐hexane fraction only contained GA (13.91 mg/100 g dry extract). Also, some other bioactive polyphenols were identified in crude and aqueous fraction extracts. The crude extract revealed superior antioxidant activity in DPPH (Half maximal inhibitory Concentration (IC50) = 149.30 ± 1.43 μg/ml) and hydroxyl radical scavenging assays (IC50 = 327.257 ± 1.36 μg/ml) in comparison to the rest of the extracts. Furthermore, all of the extracts showed dose‐dependent reducing power activity. HPLC‐identified bioactive compounds may be responsible for antioxidant activity. Because of these identified compounds, I. aquatica holds the potential feed ingredient in aquaculture.

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Bioactive polyphenol profiling and in-vitro antioxidant activity of Tinospora cordifolia Miers ex Hook F and Thoms : A potential ingredient for functional food development

Cited by 17 publications

References 11 publications

Effects of a Polyherbal Dietary Additive on Performance, Dietary Energetics, Carcass Traits, and Blood Metabolites of Finishing Lambs

Effects of a Polyherbal Dietary Additive on Performance, Dietary Energetics, Carcass Traits, and Blood Metabolites of Finishing Lambs

Identification, Validation and Standardization of Bioactive Molecules Using UPLC/MS-QToF, UHPLC and HPTLC in Divya-Denguenil-Vati: A Penta-Herbal Formulation for Dengue Fever

Profiling of bioactive compounds and antioxidant activity of aquatic weedIpomoea aquatica

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