Bioactive poly (methyl methacrylate) bone cement for the treatment of osteoporotic vertebral compression fractures

Zhu, Jinjin; Cai, Kaiwen; Wang, Shuo; Qiu, Zhiye; Huang, Jinyuan; Jiang, Guoqiang; Wang, Xiumei; Fang, Xiangqian

doi:10.7150/thno.44428

Cited by 48 publications

(34 citation statements)

References 55 publications

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“…qPCR was performed using iTaq SYBR Green supermix (172-5122; Bio-Rad, Richmond, CA, USA), and the cycles were measured using a CFX96 real-time PCR detection system (Bio-Rad). Primer sequences are listed in Table S1 , and data are presented as fold change using the 2 -△△Ct method 56 .…”

Section: Methodsmentioning

confidence: 99%

Self-assembling peptide hydrogels functionalized with LN- and BDNF- mimicking epitopes synergistically enhance peripheral nerve regeneration

et al. 2020

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The regenerative capacity of the peripheral nervous system is closely related to the role that Schwann cells (SCs) play in construction of the basement membrane containing multiple extracellular matrix proteins and secretion of neurotrophic factors, including laminin (LN) and brain-derived neurotrophic factor (BDNF). Here, we developed a self-assembling peptide (SAP) nanofiber hydrogel based on self-assembling backbone Ac-(RADA) 4 -NH 2 (RAD) dual-functionalized with laminin-derived motif IKVAV (IKV) and a BDNF-mimetic peptide epitope RGIDKRHWNSQ (RGI) for peripheral nerve regeneration, with the hydrogel providing a three-dimensional (3D) microenvironment for SCs and neurites. Methods: Circular dichroism (CD), atomic force microscopy (AFM), and scanning electron microscopy (SEM) were used to characterize the secondary structures, microscopic structures, and morphologies of self-assembling nanofiber hydrogels. Then the SC adhesion, myelination and neurotrophin secretion were evaluated on the hydrogels. Finally, the SAP hydrogels were injected into hollow chitosan tubes to bridge a 10-mm-long sciatic nerve defect in rats, and in vivo gene expression at 1 week, axonal regeneration, target muscular re-innervation, and functional recovery at 12 weeks were assessed. Results: The bioactive peptide motifs were covalently linked to the C-terminal of the self-assembling peptide and the functionalized peptides could form well-defined nanofibrous hydrogels capable of providing a 3D microenvironment similar to native extracellular matrix. SCs displayed improved cell adhesion on hydrogels with both IKV and RGI, accompanied by increased cell spreading and elongation relative to other groups. RSCs cultured on hydrogels with IKV and RGI showed enhanced gene expression of NGF, BDNF, CNTF, PMP22 and NRP2, and decreased gene expression of NCAM compared with those cultured on other three groups after a 7-day incubation. Additionally, the secretion of NGF, BDNF, and CNTF of RSCs was significantly improved on dual-functionalized peptide hydrogels after 3 days. At 1 week after implantation, the expressions of neurotrophin and myelin-related genes in the nerve grafts in SAP and Autograft groups were higher than that in Hollow group, and the expression of S100 in groups containing both IKV and RGI was significantly higher than that in groups containing either IKV or RGI hydrogels, suggesting enhanced SC proliferation. The morphometric parameters of the regenerated nerves, their electrophysiological performance, the innervated muscle weight and remodeling of muscle fibers, and motor function showed that RAD/IKV/RGI and RAD/IKV-GG-RGI hydrogels could markedly improve axonal regeneration with enhanced re-myelination and motor functional recovery through the synergetic effect of IKV and RGI functional motifs. Conclusions: We found that the dual-functionalized SAP hydrogels promoted RSC adhesion, my...

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Section: Methodsmentioning

confidence: 99%

Self-assembling peptide hydrogels functionalized with LN- and BDNF- mimicking epitopes synergistically enhance peripheral nerve regeneration

et al. 2020

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“…Reconstructed imaging of the knee joint from mice was performed using a high-resolution micro-CT instrument (InspeXio SMX-225 CT FPD HR; Shimadzu Co. Ltd., Kyoto, Japan) as previously described 56 . Briefly, an X-ray energy of 225 kV and an isotropic voxel size of 4 µm was performed on knee articular cartilage as well as distal femur and proximal tibia.…”

Section: Methodsmentioning

confidence: 99%

Novel role of circRSU1 in the progression of osteoarthritis by adjusting oxidative stress

et al. 2021

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“…In our previous study, we modified the PMMA bone cement by adding MC [ 12−16 ]. We performed in vitro and in vivo studies of MC-modified PMMA bone cement and evaluated its improvement in relation to the biological and biomechanical shortcomings of PMMA bone cement alone [ 2 , 17 ]. However, the MC-modified PMMA bone cement has not been evaluated in the treatment of KD, which has suitable modulus and bioactivity for bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

Modified poly(methyl methacrylate) bone cement in the treatment of Kümmell disease

Zhu

Yang

Teng

et al. 2021

Regenerative Biomaterials

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Kümmell disease (KD) causes serious vertebral body collapse in patients. However, only a few case reports have been conducted and the number of patients with KD investigated was limited. Additionally, the frequently used poly(methyl methacrylate) (PMMA) bone cement for KD is limited by excessive modulus and poor biocompatibility. Herein, we aimed to modify PMMA bone cement with mineralized collagen (MC), and compare the clinical effects, image performance and finite element analysis between the modified bone cement and PMMA bone cement for the treatment of phase I and II KD. Thirty-nine KD patients treated with PMMA bone cement and 40 KD patients treated with MC-modified PMMA bone cement from June 2015 to March 2017 were retrospectively analyzed. The surgical procedure, intraoperative blood loss, hospital stay and complications were compared between different groups. Visual analog scale, Oswestry disability index, anterior vertebral height, posterior vertebral height, computed tomography value, adjacent vertebral re-fracture, Cobb angle and wedge-shaped correction angle were evaluated. Additionally, the representative sample was selected for finite element analysis. We found that the MC-modified PMMA bone cement could achieve the same effect as that of PMMA bone cement and was associated with better vertebral height restoration in the long term.

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Bioactive poly (methyl methacrylate) bone cement for the treatment of osteoporotic vertebral compression fractures

Cited by 48 publications

References 55 publications

Self-assembling peptide hydrogels functionalized with LN- and BDNF- mimicking epitopes synergistically enhance peripheral nerve regeneration

Self-assembling peptide hydrogels functionalized with LN- and BDNF- mimicking epitopes synergistically enhance peripheral nerve regeneration

Novel role of circRSU1 in the progression of osteoarthritis by adjusting oxidative stress

Modified poly(methyl methacrylate) bone cement in the treatment of Kümmell disease

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