Bioactive Phenolic Compounds from Peperomia obtusifolia

Abstract

Peperomia obtusifolia (L.) A. Dietr., native to Middle America, is an ornamental plant also traditionally used for its mild antimicrobial properties. Chemical investigation on the leaves of P. obtusifolia resulted in the isolation of two previously undescribed compounds, named peperomic ester (1) and peperoside (2), together with five known compounds, viz. N-[2-(3,4-dihydroxyphenyl)ethyl]-3,4-dihydroxybenzamide (3), becatamide (4), peperobtusin A (5), peperomin B (6), and arabinothalictoside (7). The structures of these compounds were elucidated by 1D and 2D NMR techniques and HREIMS analyses. Compounds 1–7 were evaluated for their anthelmintic (against Caenorhabditis elegans), antifungal (against Botrytis cinerea, Septoria tritici and Phytophthora infestans), antibacterial (against Bacillus subtilis and Aliivibrio fischeri), and antiproliferative (against PC-3 and HT-29 human cancer cell lines) activities. The known peperobtusin A (5) was the most active compound against the PC-3 cancer cell line with IC50 values of 25.6 µM and 36.0 µM in MTT and CV assays, respectively. This compound also induced 90% inhibition of bacterial growth of the Gram-positive B. subtilis at a concentration of 100 µM. In addition, compound 3 showed anti-oomycotic activity against P. infestans with an inhibition value of 56% by using a concentration of 125 µM. However, no anthelmintic activity was observed.

Characterization and Bioactive Potential of Secondary Metabolites Isolated from Piper sarmentosum Roxb.

“…The isolated compounds were tested at concentrations of 1 and 100 μM against the Gram-negative Aliivibrio fischeri test strain DSM507 (batch no. 1209), with chloramphenicol (100 μM) serving as a positive control as previously reported [ 3 , 93 ].…”

“…It consists of five genera namely Verhuellia, Zippelia, Manekia, Piper and Peperomia [ 1 ]. The most frequently described genera are Piper and Peperomia [ 1 , 2 , 3 ]. The Piper genus contains about 1000–2000 species with dominant species in their native habitat [ 4 ].…”

Alpha-Glucosidase Inhibitor Activities and Phytochemicals Screening of the Peperomia Genus Cultivated in Indonesia

“…In the study five ethanol extract presence of flavonoids, phenols, quinone, and saponins (table 1). Recently new phenolic compound from P. obtusifolia has been reported; they are peperomic ester and peperoside [21].…”

Comparison of Balanites aegyptiaca parts: metabolome providing insights into plant health benefits and valorization purposes as analyzed using multiplex GC-MS, LC-MS, NMR-based metabolomics, and molecular networking

“…Finally, the formazan absorbance was measured at 570 nm (and background at 670 nm for data normalization) by using a SpectraMax iD5 multi-well plate reader (Molecular Devices, San Jose, CA, USA). 23 For CV assay, cells were washed with 50 μL per well of PBS, followed by cell fixation using 4% (w/v) PFA in PBS for 10 min. After fixation, the PFA solution was discarded, and the plates were dried for another 10 min.…”