Bioactive peptides derived fromRadix Angelicae sinensisinhibit ferroptosis in HT22 cells through direct Keap1–Nrf2 PPI inhibition

Abstract

Antioxidant peptides derived from Radix Angelicae sinensis can inhibit ferroptosis by directly inhibiting Keap1–Nrf2 PPI.

Database‐aided ultrahigh‐performance liquid chromatography Q‐Exactive‐Orbitrap tandem mass spectrometry putatively identifies 16 unexpected compounds and three anticounterfeiting pharmacopoeia quality markers for Perillae Fructus

“…39 It is widely accepted that a more negative binding energy indicates a more stable complex. 40 Thus, the negative binding energy values demonstrate that all five compounds could interact with AMCase, with compounds 1, 8, and 17 binding to AMCase more readily than the standard drug of salbutamol (Table 3). 40 The binding modes reveal that matrine occupies a spacious cavity and interacts with active amino acid residues, including TRP-99, TYR-212, ASP-213, TYR-267, ILE-300, and TRP-360, through hydrophobic interactions, hydrogen bonds, and salt bridges (Figure 4).…”

“…40 Thus, the negative binding energy values demonstrate that all five compounds could interact with AMCase, with compounds 1, 8, and 17 binding to AMCase more readily than the standard drug of salbutamol (Table 3). 40 The binding modes reveal that matrine occupies a spacious cavity and interacts with active amino acid residues, including TRP-99, TYR-212, ASP-213, TYR-267, ILE-300, and TRP-360, through hydrophobic interactions, hydrogen bonds, and salt bridges (Figure 4). Similarly, scopolamine and RosA bind to AMCase through noncovalent interactions with residues TRP-31, PHE-58, TRP-99, ASN-100, ASP-213, and TRP-360.…”