Bioactive peptide functionalized aligned cyclodextrin nanofibers for neurite outgrowth

Hamsici, Seren; Çınar, Göksu; Çelebioğlu, Aslı; Uyar, Tamer; Tekinay, Ayşe B.; Güler, Mustafa O.

doi:10.1039/c6tb02441f

Cited by 38 publications

(21 citation statements)

References 46 publications

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“…The most basic requirement for a synthetic biomaterial scaffold for cell growth and tissue regeneration (beyond toxicity) is its ability to support cell attachment (Cooke et al, 2010). For this purpose, synthetic biomaterials for neuronal regeneration are typically coated or functionalized with either (a) polymers which are able to interact with the negatively charged cell membrane, (b) reactive layers that can (covalently) bind to the cell surface, or (c) specific cell adhesive molecules able to interact with adhesive receptors at the cell membrane (i.e., integrins) Lu et al, 2014;Akizawa et al, 2016;Hamsici et al, 2017). Positively charged polymers like polylysine (PL), poly(ornithine) (PO), poly(ethyleneimine) (PEI), poly(propyleneimine) (PPI) or poly(allylamine hydrochloride) are traditionally used in neuronal cell cultures (Roach et al, 2010).…”

Section: Biomaterials That Support Neuronal Cell Adhesionmentioning

confidence: 99%

Microenvironments Designed to Support Growth and Function of Neuronal Cells

2018

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Strategies for neural tissue repair heavily depend on our ability to temporally reconstruct the natural cellular microenvironment of neural cells. Biomaterials play a fundamental role in this context, as they provide the mechanical support for cells to attach and migrate to the injury site, as well as fundamental signals for differentiation. This review describes how different cellular processes (attachment, proliferation, and (directional) migration and differentiation) have been supported by different material parameters, in vitro and in vivo. Although incipient guidelines for biomaterial design become visible, literature in the field remains rather phenomenological. As in other fields of tissue regeneration, progress will depend on more systematic studies on cell-materials response, better understanding on how cells behave and understand signals in their natural milieu from neurobiology studies, and the translation of this knowledge into engineered microenvironments for clinical use.

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Section: Biomaterials That Support Neuronal Cell Adhesionmentioning

confidence: 99%

Microenvironments Designed to Support Growth and Function of Neuronal Cells

2018

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“…To reveal the aggregation behavior of the PAs, the critical aggregation concentrations (CACs) of the molecules were first studied using a Nile Red assay. 59 The CACs of the PAs were determined as being higher than approximately 1 μM in water ( Figure S5). Hence, all structural studies were carried out above this concentration to obtain histidine-functionalized PA assemblies under slightly acidic (pH 4.5), neutral (pH 7.4), and basic (pH 10) conditions.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Hierarchical Self-Assembly of Histidine-Functionalized Peptide Amphiphiles into Supramolecular Chiral Nanostructures

et al. 2017

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Controlling the hierarchical organization of self-assembling peptide amphiphiles into supramolecular nanostructures opens up the possibility of developing biocompatible functional supramolecular materials for various applications. In this study, we show that the hierarchical self-assembly of histidine- (His-) functionalized PAs containing d- or l-amino acids can be controlled by both solution pH and molecular chirality of the building blocks. An increase in solution pH resulted in the structural transition of the His-functionalized chiral PA assemblies from nanosheets to completely closed nanotubes through an enhanced hydrogen-bonding capacity and π-π stacking of imidazole ring. The effects of the stereochemistry and amino acid sequence of the PA backbone on the supramolecular organization were also analyzed by CD, TEM, SAXS, and molecular dynamics simulations. In addition, an investigation of chiral mixtures revealed the differences between the hydrogen-bonding capacities and noncovalent interactions of PAs with d- and l-amino acids.

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“…Hence, trophic factors (Nguyen et al, ) and peptide sequences (Callahan et al, ) have been incorporated on aligned electrospun nanofibers to enhance the cell fate processes. Laminin‐derived bioactive isoleucine‐lysine‐valine‐alanine‐valine (IKVAV) epitope was combined with electrospun cyclodextrin aligned nanofibers to promote neuronal outgrowth and differentiation (Hamsici et al, ). Risk of denaturation during fabrication, requirement of additional cross linking to maintain scaffold stability, immunogenicity concerns (Callahan et al, ; Nune, Kumaraswamy, Krishnan, & Sethuraman, ; Wang et al, ) are the other potential demerits associated with the use of natural polymers.…”

Section: Introductionmentioning

confidence: 99%

Peptide nanostructures on nanofibers for peripheral nerve regeneration

Nune

Subramanian

Krishnan

et al. 2019

J Tissue Eng Regen Med

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Self‐assembled peptide nanofibrous scaffolds with designer sequences, similar to neurite growth promoting molecules enhance the differentiation of neural stem cells. However, self‐assembled peptide nanofibrous scaffolds lack the required mechanical strength to suffice to bridge long critical‐sized peripheral nerve defects. Hence, there is a demand for a potential neural substrate, which could be biomimetic coupled with bioactive nanostructures to regrow the denuded axons towards the distal end. In the present study, we developed designer self‐assembling peptide‐based aligned poly(lactic‐co‐glycolic acid) (PLGA) nanofibrous scaffolds by simple surface coating of peptides or coelectrospinning. Retention of secondary structures of peptides in peptide‐coated and cospun fibers was confirmed by circular dichroism spectroscopy. The rod‐like peptide nanostructures enhance the typical bipolar morphology of Schwann cells. Although the peptide‐coated PLGA scaffolds exhibited significant increase in Schwann cell proliferation than pristine PLGA and PLGA‐peptide cospun scaffolds (p < .05), peptide cospun scaffolds demonstrated better cellular infiltration and significantly higher gene expression of neural cell adhesion molecule, glial fibrillary acidic protein, and peripheral myelin protein22 compared to the pristine PLGA and PLGA‐peptide‐coated scaffolds. Our results demonstrate the positive effects of aligned peptide coelectrospun scaffolds with biomimetic cell recognition motifs towards functional proliferation of Schwann cells. These scaffolds could subsequently repair peripheral nerve defects by augmenting axonal regeneration and functional nerve recovery.

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Bioactive peptide functionalized aligned cyclodextrin nanofibers for neurite outgrowth

Cited by 38 publications

References 46 publications

Microenvironments Designed to Support Growth and Function of Neuronal Cells

Microenvironments Designed to Support Growth and Function of Neuronal Cells

Hierarchical Self-Assembly of Histidine-Functionalized Peptide Amphiphiles into Supramolecular Chiral Nanostructures

Peptide nanostructures on nanofibers for peripheral nerve regeneration

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