Bioactive molecules of probiotic bacteria and their mechanism of action: a review

Indira, M.; Venkateswarulu, T.C.; Peele, K. Abraham; Bobby, Nazneen; Krupanidhi, S.

doi:10.1007/s13205-019-1841-2

Cited by 83 publications

(58 citation statements)

References 109 publications

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“…Some studies have demonstrated that probiotic strains may exert beneficial effects on H. pylori infection and inflammation through the molecules produced and secreted by bacterial cells [56]. Interestingly, it was reported that L. rhamnosus GG-conditioned media was able to antagonize TNF-α secretion induced by H. pylori or LPS in murine macrophages [57].…”

Section: Discussionmentioning

confidence: 99%

The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection

et al. 2020

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Lactobacillus fermentum UCO-979C (Lf979C) beneficially modulates the cytokine response of gastric epithelial cells and macrophages after Helicobacter pylori infection in vitro. Nevertheless, no in vivo studies were performed with this strain to confirm its beneficial immunomodulatory effects. This work evaluated whether Lf979C improves protection against H. pylori infection in mice by modulating the innate immune response. In addition, we evaluated whether its exopolysaccharide (EPS) was involved in its beneficial effects. Lf979C significantly reduced TNF-α, IL-8, and MCP-1 and augmented IFN-γ and IL-10 in the gastric mucosa of H. pylori-infected mice. The differential cytokine profile induced by Lf979C in H. pylori-infected mice correlated with an improved reduction in the pathogen gastric colonization and protection against inflammatory damage. The purified EPS of Lf979C reduced IL-8 and enhanced IL-10 levels in the gastric mucosa of infected mice, while no effect was observed for IFN-γ. This work demonstrates for the first time the in vivo ability of Lf979C to increase resistance against H. pylori infection by modulating the gastric innate immune response. In addition, we advanced knowledge of the mechanisms involved in the beneficial effects of Lf979C by demonstrating that its EPS is partially responsible for its immunomodulatory effect.

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Section: Discussionmentioning

confidence: 99%

The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection

et al. 2020

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“…ADS activity contributes to pH homeostasis by generating alkali from L-arginine, thereby reducing the ecological advantage of aciduric pathogens while preventing dental hard tissue demineralization [ 10 , 11 ]. Direct inhibition of pathogens has been demonstrated via competing for common nutrients or by inducing the production of inhibitory substances, i.e., bacteriocins and metabolic products, or by co-aggregation with cariogenic organisms [ 12 , 13 , 14 ]. Additionally, probiotic organisms have been shown to compete for binding sites on surfaces or host cells and thus, exclude the adherence of pathogenic bacteria [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Probiotic Effects on Multispecies Biofilm Composition, Architecture, and Caries Activity In Vitro

et al. 2020

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While probiotics have been tested for their anti-caries effect in vitro and also clinically, there is a lack of understanding of their effects on complex dental biofilms. We assessed two probiotics, Lactobacillus reuteri and Streptococcus oligofermentans, on a continuous-cultured model containing Streptococcus mutans, Lactobacillus rhamnosus and Actinomyces naeslundii. Cariogenic biofilms were grown on bovine enamel specimens and daily challenged with L. reuteri or S. oligofermentans whole culture (LC/SC) or cell-free supernatant (LS/SS) or medium only (negative control, NC) (n = 21/group) for 10 days. Biofilm was assessed via counting colony-forming units, quantitative polymerase chain reaction, and fluorescence in situ hybridization. Caries activity was determined by pH measurements and by assessing mineral loss (ΔZ) using transverse microradiography. Both LC and SC significantly reduced total and strain-specific cariogenic bacterial numbers (p < 0.05). ΔZ was reduced in LC (mean ± SD: 1846.67 ± 317.89) and SC (3315.87 ± 617.30) compared to NC (4681.48 ± 495.18, p < 0.05). No significant reductions in bacterial numbers and ΔZ was induced by supernatants. Biofilm architecture was not considerably affected by probiotic applications. Viable probiotics L. reuteri and S. oligofermentans, but not their culture supernatants, could reduce the caries activity of multi-species biofilms in vitro.

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“…This is accomplished by displacing pathogens (two bodies cannot occupy the same place at the same time), competing for nutrients, competing for receptors, and producing factors that can interfere with the survival of pathobiont strains. For example, the good bacteria secrete bacteriocins (natural antibiotics) and other colicins, and they generate -from the fermentation of nondigestible carbohydrates -short-chain fatty acids (SCFAs) such as butyrate, propionate, lactate, and acetate, which reduce colonic pH, thus hindering bacterial proliferation (16)(17)(18)(19)(20) .…”

Section: Introductionmentioning

confidence: 99%

Gut Microbiota, Prebiotics, Probiotics, and Synbiotics in Gastrointestinal and Liver Diseases: Proceedings of a Joint Meeting of the Brazilian Society of Hepatology (Sbh), Brazilian Nucleus for the Study of Helicobacter Pylori and Microbiota (Nbehpm), and Brazilian Federation of Gastroenterology (Fbg)

Schiavon¹,

Oliveira

Álvares‐da‐Silva

et al. 2020

Arq. Gastroenterol.

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Over the last years, there is growing evidence that microorganisms are involved in the maintenance of our health and are related to various diseases, both intestinal and extraintestinal. Changes in the gut microbiota appears to be a key element in the pathogenesis of hepatic and gastrointestinal disorders, including non-alcoholic fatty liver disease, alcoholic liver disease, liver cirrhosis, inflammatory bowel disease, irritable bowel syndrome, and Clostridium difficile - associated diarrhea. In 2019, the Brazilian Society of Hepatology (SBH) in cooperation with the Brazilian Nucleus for the Study of Helicobacter Pylori and Microbiota (NBEHPM), and Brazilian Federation of Gastroenterology (FBG) sponsored a joint meeting on gut microbiota and the use of prebiotics, probiotics, and synbiotics in gastrointestinal and liver diseases. This paper summarizes the proceedings of the aforementioned meeting. It is intended to provide practical information about this topic, addressing the latest discoveries and indicating areas for future studies.

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Bioactive molecules of probiotic bacteria and their mechanism of action: a review

Cited by 83 publications

References 109 publications

The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection

The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection

Probiotic Effects on Multispecies Biofilm Composition, Architecture, and Caries Activity In Vitro

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