Bioactive Lipids in MSCs Biology: State of the Art and Role in Inflammation

Casati, Sara; Giannasi, Chiara; Niada, Stefania; Bergamaschi, Roberta F; Orioli, Marica; Brini, A.T.

doi:10.3390/ijms22031481

Cited by 11 publications

(5 citation statements)

References 167 publications

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“…This is in accordance with the theory that foods containing animal fats in the body turn into eicosanoids, in limited quantities. This substance is needed by the body, but if the levels of eicosanoids exceed normal limits it can cause joint disease [17,18].…”

Section: Discussionmentioning

confidence: 99%

Range of Motion (ROM) Exercise Reduce Joint Pain in the Elderly

Indriawati¹,

Sossa²

2022

Proceedings of the International Conference on Sustainable Innovation on Health Sciences and Nursing (ICOSI-HSN 2022)

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Introduction -Elderly is a time when a person will experience a decrease in body functions both physically, mentally and socially. One of the physical declines felt by the elderly is the decline in joint movement. The reduced elasticity of the joints will make stiffness in the joints resulting in joint pain. Range of motion (ROM) is one of the activities that can be used to relieve joint pain.Purpose -The purpose of this study was to determine the effect of joint motion exercises (ROM) on joint pain felt in the elderly.Methodology/Approach -This study used a quasi-experimental pre-post-test in the treatment group and the control group. The number of subjects was 52 people with 26 people in the treatment group and 26 people in the control group. The treatment group was given joint motion exercises 3x/week for 6 weeks. The statistical test in the treatment group used the paired sample T-Test, the control group used the Wilcoxon signed rank test and for the comparison test, the Mann Whitney test was used.Results -The results of the study in the treatment group showed that ROM had an effect on reducing joint pain in the elderly (p = 0.001), in the control group there was no significant change (p = 0.265) There was a difference in the effect of reducing joint pain in the treatment group and the control group (p = 0.001).Conclusion -ROM exercises can decrease joint pain in the elderly. .

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Section: Discussionmentioning

confidence: 99%

Range of Motion (ROM) Exercise Reduce Joint Pain in the Elderly

Indriawati¹,

Sossa²

2022

Proceedings of the International Conference on Sustainable Innovation on Health Sciences and Nursing (ICOSI-HSN 2022)

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“…Among quantified lipids, SEA and PGE2 showed a relevant uniformity that was therefore validated in a larger ASC-CM cohort. SEA is an endogenous lipid belonging to the N-acylethanolamides family that acts as an anti-inflammatory/immunomodulatory agent through the downregulation of several proinflammatory cytokines [ 37 ]. Conversely, PGE2 exerts a well-known inflammatory action.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, in the OA context, it can exert either anabolic or catabolic effects on chondrocytes and synoviocytes depending on its concentration [ 38 – 40 ]. Evidence suggests also that PGE2 may have an immune stimulatory role by facilitating Th1 differentiation and expanding Th17 T cells [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Towards Secretome Standardization: Identifying Key Ingredients of MSC-Derived Therapeutic Cocktail

Giannasi

Niada

Morte

et al. 2021

Stem Cells International

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The therapeutic potential of the conditioned medium (CM) derived from MSCs (mesenchymal stem/stromal cells) in disparate medical fields, from immunology to orthopedics, has been widely suggested by in vitro and in vivo evidences. Prior to MSC-CM use in clinical applications, appropriate quality controls are needed in order to assess its reproducibility. Here, we evaluated different CM characteristics, including general features and precise protein and lipid concentrations, in 3 representative samples from adipose-derived MSCs (ASCs). In details, we first investigated the size and distribution of the contained extracellular vesicles (EVs), lipid bilayer-delimited particles whose pivotal role in intercellular communication has been extensively demonstrated. Then, we acquired Raman signatures, providing an overlook of ASC-CM composition in terms of proteins, lipids, and nucleic acids. At last, we analyzed a panel of 200 molecules including chemokines, cytokines, receptors, and inflammatory and growth factors and searched for 32 lipids involved in cell signalling and inflammation. All ASC-CM contained a homogeneous and relevant number of EVs ( 1.0 × 10 9 ± 1.1 × 10 8 particles per million donor ASCs) with a mean size of 190 ± 5.2 nm , suggesting the appropriateness of the method for EV retaining and concentration. Furthermore, also Raman spectra confirmed a high homogeneity among samples, allowing the visualization of specific peaks for nucleic acids, proteins, and lipids. An in depth investigation that focused on 200 proteins involved in relevant biological pathways revealed the presence in all specimens of 104 factors. Of these, 26 analytes presented a high degree of uniformity, suggesting that the samples were particularly homogenous for a quarter of the quantified molecules. At last, lipidomic analysis allowed the quantification of 7 lipids and indicated prostaglandin-E2 and N-stearoylethanolamide as the most homogenous factors. In this study, we assessed that ASC-CM samples obtained with a standardized protocol present stable features spanning from Raman fingerprint to specific marker concentrations. In conclusion, we identified key ingredients that may be involved in ASC-CM therapeutic action and whose consistent levels may represent a promising quality control in the pipeline of its preparation for clinical applications.

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“…A study for single-cell analysis to closely monitor the cell signaling and change in morphological phenotypes can yield further insights into the mechanisms and implications of single-cell variation amongst these cells and can help enhance their clinical efficacy. There is growing evidence that lipids play a vital role in MSC functions and are closely associated with the MSCs morphological phenotype 6,7 . Although lipid metabolism plays a pivotal role in MSCs physiopathology, the number of studies at the moment about the lipidome of MSCs is limited and no strong link has been established to understand the direct relationships between single-cell MSC lipidome and its phenotype.…”

Section: Introductionmentioning

confidence: 99%

Integration of imaging modalities with lipidomic characterization to investigate MSCs potency metrics

Priyadarshani

Grouw²,

Liversage

et al. 2022

Preprint

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Mesenchymal stem cells (MSCs) are widely used as therapeutics targets for numerous autoimmune diseases. However, MSC therapies have had limited success so far in clinical trials, mainly being heterogenous population it is difficult to determine MSCs efficiencies. It is critical to understand internal signaling of individual MSCs population that directly affect the cell phenotype. Lipid signaling is closely associated with cell shape so, a holistic approach to understand how changes in lipid metabolites trickles all the way to single cell phenotype could reveal deeper understanding of MSCs functional regulation. So, we aim to evaluate lipid metabolic profiles of single cell MSCs with known variability in immune regulation and explore the phenotypic changes that occur because of differences in lipid signaling. We use longitudinal label free phase imaging strategies to obtain cell phenotypic features which are directly correlated with single cell lipid metabolome obtained using advanced MALDI-MSI technique. Correlation maps indicate associations between lipid signaling and phenotypic changes in MSCs.Moreover, a novel machine learning clustering approach detects the heterogeneity in the MSCs subpopulation then methodically see how each heterogenous population is being impacted by the changes in lipid profiles which could be linked to the functional behaviors of the cell.

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Bioactive Lipids in MSCs Biology: State of the Art and Role in Inflammation

Cited by 11 publications

References 167 publications

Range of Motion (ROM) Exercise Reduce Joint Pain in the Elderly

Range of Motion (ROM) Exercise Reduce Joint Pain in the Elderly

Towards Secretome Standardization: Identifying Key Ingredients of MSC-Derived Therapeutic Cocktail

Integration of imaging modalities with lipidomic characterization to investigate MSCs potency metrics

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