Bioactive Hydrogel Marbles

Leite, Álvaro J.; Oliveira, Nuno M.; Song, Wenlong; Mano, João F.

doi:10.1038/s41598-018-33192-6

Cited by 13 publications

(8 citation statements)

References 70 publications

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“…The Ca:P ratio obtained for the MSN‐CaP samples have values close to that of hydroxyapatite (1.67) (Figure 3E). [ 40 ] The bioactivity was also further assessed through XRD diffractograms (Figure 3F) and FTIR spectroscopy (Figure 3G). In the XRD diffractograms, the peaks corresponding to hydroxyapatite are present in MSN‐CaP samples, but not in the control nanoparticles (bare MSNs).…”

Section: Resultsmentioning

confidence: 99%

Efficient Single‐Dose Induction of Osteogenic Differentiation of Stem Cells Using Multi‐Bioactive Hybrid Nanocarriers

et al. 2020

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such as limited bone supply, donor site morbidity, risk of infection, or loss of function. [2,3] Synthetic permanent bone substitution grafts are alternative options, however, those show poor osteointegration, may release toxic ions, and present differences in the mechanical properties at the bone/prosthesis interface that can give rise to ruptures. [1,4] Bone tissue engineering offers an alternative approach, with the potential to repair critical size defects and promote bone regeneration. Although different nanomaterials with osteocompatible, osteoconductive, or osteoinductive effects have been previously described in the literature, [5-8] here we propose an original multifunctional approach based on a hybrid silica nanomaterial that can release calcium and phosphate ions intracellularly, and simultaneously deliver a pro-osteogenic substance, dexamethasone (Dex). We hypothesize that the simultaneous intracellular delivery of different stimuli will result in synergistic effects that allow a scaffold-free strategy to induce the differentiation of stem cells into osteoblasts. Silica nanomaterials (and their degradation products) are biocompatible and "generally recognized as safe" (GRAS) by the U.S. Food and Drug Administration (FDA). [9] The degradation rate of silica nanoparticles depends on their size, porosity, and other parameters, [10] and it has been shown that mesoporous silica nanoparticles (MSNs) with 180 nm diameter degraded over 50% in 3 d, and nearly 100% in 7 d. [11] Large size silicabased bioactive materials have been used in orthopedic applications, [12,13] because they are biocompatible, [14] biodegradable, [15] osteoconductive, [16] and even angiogenic. [13,15] Silica released from these materials, together with calcium and phosphate ions, originate both intracellular and extracellular responses that activate the cells in contact with this stimulus. [15,17,18] Previous studies showed how calcium ions stimulate osteoblast proliferation and differentiation, while phosphate ions enhance the rate of bone matrix production by osteogenic cells. [19,20] This spatiotemporally controlled release also induces the formation of a hydroxyapatite layer, which allows bonding to the bone tissue, hence improving osteointegration. [15,21] This process can be greatly improved by using nanosized bioactive materials, which due to their large specific surface area Bone regeneration requires the presence of specific factors to induce the differentiation of stem cells into osteoblasts. These factors induce osteogenesis by stimulating the expression of bone-related proteins, bone cell proliferation and differentiation. Herein, bioactive mesoporous silica nanoparticles are doped with calcium and phosphate ions while the porous network is loaded with dexamethasone (MSN-CaPDex). The bioactive MSN-CaPDex nanocarriers are prepared without affecting the narrow size distribution, pore structure, and morphology of the MSNs, while incorporating multi-stimuli, complementary ionic/biochemical bioactive mediators. The bioactive nano...

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Section: Resultsmentioning

confidence: 99%

Efficient Single‐Dose Induction of Osteogenic Differentiation of Stem Cells Using Multi‐Bioactive Hybrid Nanocarriers

et al. 2020

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“…The sustained shaking of the LMs is inevitable, which may interfere the inner liquid reactions especially for the cell activities in the biomedical applications. Recently, researchers have applied the LM to culture cells in the 3D format by manually rolling the medium on a hydrophobic powder . However, the proposed method applied a tedious manual culture process and the sustained supply of nutrients and medium during medium renewal was difficult to achieve, which hampered the long‐time culture of the 3D cell spheroid.…”

Section: Introductionmentioning

confidence: 99%

On‐Demand Coalescence and Splitting of Liquid Marbles and Their Bioapplications

Wang

Chan

et al. 2019

Advanced Science

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Coalescence and splitting of liquid marbles (LMs) are critical for the mixture of precise amount precursors and removal of the wastes in the microliter range. Here, the coalescence and splitting of LMs are realized by a simple gravity‐driven impact method and the two processes are systematically investigated to obtain the optimal parameters. The formation, coalescence, and splitting of LMs can be realized on‐demand with a designed channel box. By selecting the functional channels on the device, gravity‐based fusion and splitting of LMs are performed to mix medium/drugs and remove spent culture medium in a precise manner, thus ensuring that the microenvironment of the cells is maintained under optimal conditions. The LM‐based 3D stem cell spheroids are demonstrated to possess an approximately threefold of cell viability compared with the conventional spheroid obtained from nonadhesive plates. Delivery of the cell spheroid to a hydrophilic surface results in the in situ respreading of cells and gradual formation of typical 2D cell morphology, which offers the possibility for such spheroid‐based stem cell delivery in regenerative medicine.

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“…As an alternative DMF platform, LMs can effectively serve as an individual compartment to provide various microfluidic functions. Recently, LMs have been innovatively used as microreactors for chemical [10][11][12] and biomedical [13][14][15] analysis. The three-dimensional geometry, low risk of cross-contamination, and gas permeability make a LM the perfect candidate for culturing * nam-trung.nguyen@griffith.edu.au cells [16][17][18] and microorganisms [19].…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic Trapping of a Floating Liquid Marble

et al. 2019

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A liquid marble, a liquid droplet coated with hydrophobic powder, is an emerging digital microfluidic platform. It can potentially serve as a mixer or reactor for chemical and biological assays in the microscale. Automated manipulation of liquid marbles is essential for the implementation of more microfluidic functions in the technology platform of "lab in a marble". We report the analytical and experimental results of trapping a floating liquid marble using dielectrophoresis in a nonuniform electric field. Liquid marbles with volumes ranging from 5 to 50 μL are effectively trapped by the dielectrophoretic force from a horizontal distance ranging from 10 to 60 mm and under an applied voltage ranging from 1.6 to 5 kV. A one-dimensional analytical model is then proposed for the trapping process and agrees well with experimental data. Finally, based on the relationship between the static friction coefficient and the Bond number of a floating liquid marble, an operation map is derived for successful trapping of the liquid marble, providing a better insight into controlled manipulation of liquid marbles.

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Bioactive Hydrogel Marbles

Cited by 13 publications

References 70 publications

Efficient Single‐Dose Induction of Osteogenic Differentiation of Stem Cells Using Multi‐Bioactive Hybrid Nanocarriers

Efficient Single‐Dose Induction of Osteogenic Differentiation of Stem Cells Using Multi‐Bioactive Hybrid Nanocarriers

On‐Demand Coalescence and Splitting of Liquid Marbles and Their Bioapplications

Dielectrophoretic Trapping of a Floating Liquid Marble

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