Bioactive glasses induce chemiluminescence by human polymorphonuclear leukocytes

Abstract

The effect of bioactive glasses on human polymorphonuclear leukocytes (PMNLs) were studied in vitro by a chemiluminescence (CL) assay. Eight different glasses were chosen. All glasses induced a rapid CL response by human PMNLs, which proved to be dose dependent. The CL response also seemed to depend on the durability of the glasses. The least durable glass caused the highest CL response, and highly durable glasses caused only low CL responses by the cells.

Immunoglobulin enhances the bioactive-glass-induced chemiluminescence response of human polymorphonuclear leukocytes

“…In a previous study we demonstrated that bioactive glasses can induce release of ROMs by PMNLs. 8 In this study it is demonstrated that the response of PMNLs to HA crystals is low compared to that of the bioactive glass.…”

“…The final fraction of glass consisted mostly of particles < 5 m and was obtained by using the gravimetric sedimentation method. 8 A mixture of glass powder and ethanol was shaken in a test tube, and after sedimentation for 1 min, the heaviest particles were separated by pipetting 90% of the dispersion. The dispersion was moved to another test tube filled with ethanol.…”

“…In a previous study we have shown that bioactive glasses cause activation of human polymorphonuclear leukocytes (PMNL) in vitro. 8 This activation of inflammatory cells, induced by particle-to-cell-membrane contact and phagocytosis, leads to an increase in oxygen uptake, O 2˙− , OH˙, and H 2 O 2 production, and hexose monophosphate shunt activity. Opsonized bacteria, crystal and mineral dusts, as well as surface-modified TiO 2 can activate the release of ROMs.…”