Bioactive Glass Nanoparticles-Loaded Poly(ɛ-caprolactone) Nanofiber as Substrate for ARPE-19 Cells

Abstract

Bioactive glass nanoparticles-loaded poly(ɛ-caprolactone) nanofibers (BIOG PCL nanofibers) were synthesized and evaluated as substrates for ocular cells (ARPE-19). BIOG PCL nanofibers were characterized using SEM, FTIR, and DSC, and thein vitrodegradation profile was also investigated. Thein vitroocular biocompatibility of nanofibers was exploited in Müller glial cells (MIO-M1 cells) and in chorioallantoic membrane (CAM); and the proliferative capacity, cytotoxicity, and functionality were evaluated. Finally, ARPE-19 cells were seeded onto BIOG PCL nanofibers and they were investigated as supports forin vitrocell adhesion and proliferation. SEM images revealed the incorporation of BIOG nanoparticles into PCL nanofibers. Nanoparticles did not induce modifications in the chemical structure and semicrystalline nature of PCL in the nanofiber, as shown by FTIR and DSC. MIO-M1 cells exposed to BIOG PCL nanofibers showed viability, and they were able to proliferate and to express GFAP, indicating cellular functionality. Moreover, nanofibers were well tolerated by CAM. These findings suggested thein vitroocular biocompatibility and absence of toxicity of these nanofibers. Finally, the BIOG nanoparticles modulated the protein adsorption, and, subsequently, ARPE-19 cells adhered and proliferated onto the nanostructured supports, establishing cell-substrate interactions. In conclusion, the biodegradable and biocompatible BIOG PCL nanofibers supported the ARPE-19 cells.

Electrospun nano-fibrous bilayer scaffold prepared from polycaprolactone/gelatin and bioactive glass for bone tissue engineering

“…When the effect of BG on viscosity was greater than that of conductivity, the average fiber diameter increased as shown by the PCL/Gt/BG composite nanofibers. However, below a certain BG concentration, observed fiber diameters decreased with the addition of BG nanoparticles, as their effect on conductivity surpassed that of viscosity [ 22 , 37 ]. The incorporation of BG nanoparticles induced a rough surface morphology, which was also reported by Lima et al [ 37 ].…”

“…However, below a certain BG concentration, observed fiber diameters decreased with the addition of BG nanoparticles, as their effect on conductivity surpassed that of viscosity [ 22 , 37 ]. The incorporation of BG nanoparticles induced a rough surface morphology, which was also reported by Lima et al [ 37 ]. This surface roughness is preferable for cellular adhesion and proliferation [ 38 ].…”

“…In this study, nanonets appeared in all scaffolds which was likely due to the high conductivity of formic acid, owing to its high dielectric constant (ε = 57.5). Moreover, BG increased solution conductivity, producing fibers of smaller diameter, which explains the increase in the distribution of nanonets in BG-containing scaffolds [ 37 ]. Similar nanonets were previously noted, as it was reported that their presence increased the scaffold’s mechanical properties, surface area and enhanced pore interconnectivity [ 40 , 41 ].…”

“…The PCL-L2 bilayer scaffold showed significant weight gain after 7 days of immersion, which could be due to amorphous calcium phosphate deposition, which led to a net gain in weight. This was followed by progressive scission of the polymeric chains due to the increase in water uptake by the scaffold [ 37 ]. PCL/Gt-L2 bilayer scaffold showed a steady rate of degradation between 2 and 14 days, while the PCL/Gt/BG composite scaffold reached its maximum weight loss at 7 days.…”

Sustainable drug release from highly porous and architecturally engineered composite scaffolds prepared by 3D printing

“…FTIR spectrum of BG nanoparticles (Figure d) demonstrated a main broad band between 1,200 cm −1 and 900 cm −1 corresponding to Si‐O‐Si networks, which could be overlapped with the infrared band of P‐O symmetric stretching vibration (Henrique Lima et al, ; Yannas, Lee, Orgill, Skrabut, & Murphy, ). The other band at ∼1,500 cm −1 corresponded to the organics such as alkoxy groups (Yannas et al, ).…”