Bioactive Functions of Milk Proteins: a Comparative Genomics Approach

Sharp, Julie A.; Modepalli, Vengamanaidu; Enjapoori, Ashwantha Kumar; Bisana, Swathi; Abud, Helen E.; Lefèvre, Christophe; Nicholas, Kevin R.

doi:10.1007/s10911-015-9331-6

Cited by 28 publications

(31 citation statements)

References 144 publications

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“…In contrast, in the majority of eutherian species, lung develops prior to birth, particularly in response to the placenta which regulates intrauterine fetal development by supplying various bioactives (Bell et al, 1999, Garnica and Chan, 1996) supporting fetal respiration (Sibley et al, 1997). Unlike eutherians, the marsupial newborn development relies on factors supplied through milk (Nicholas et al, 1997, Sharp et al, 2014). Based on our previous study (Modepalli et al, 2015), we observed the milk protein collected from different time points of early lactation had temporally related effects on embryonic mouse lung in vitro .…”

Section: Discussionmentioning

confidence: 99%

Marsupial tammar wallaby delivers milk bioactives to altricial pouch young to support lung development

Modepalli

Hinds

Sharp

et al. 2016

Mechanisms of Development

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Our research is exploiting the marsupial as a model to understand the signals required for lung development. Marsupials have a unique reproductive strategy, the mother gives birth to altricial neonate with an immature lung and the changes in milk composition during lactation in marsupials appears to provide bioactives that can regulate diverse aspects of lung development, including branching morphogenesis, cell proliferation and cell differentiation. These effects are seen with milk collected between 25 and 100 days postpartum. To better understand the temporal effects of milk composition on postnatal lung development we used a cross-fostering technique to restrict the tammar pouch young to milk composition not extending beyond day 25 for 45 days of its early postnatal life. These particular time points were selected as our previous study showed that milk protein collected prior to ~ day 25 had no developmental effect on mouse embryonic lungs in culture. The comparative analysis of the foster group and control young at day 45 postpartum demonstrated that foster pouch young had significantly reduced lung size. The lungs in fostered young were comprised of large intermediate tissue, had a reduced size of airway lumen and a higher percentage of parenchymal tissue. In addition, expression of marker genes for lung development (BMP4, WNT11, AQP-4, HOPX and SPB) were significantly reduced in lungs from fostered young. Further, to identify the potential bioactive expressed by mammary gland that may have developmental effect on pouch young lungs, we performed proteomics analysis on tammar milk through mass-spectrometry and listed the potential bioactives (PDGF, IGFBP5, IGFBPL1 and EGFL6) secreted in milk that may be involved in regulating pouch young lung development. The data suggest that postnatal lung development in the tammar young is most likely regulated by maternal signalling factors supplied through milk.

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Section: Discussionmentioning

confidence: 99%

Marsupial tammar wallaby delivers milk bioactives to altricial pouch young to support lung development

Modepalli

Hinds

Sharp

et al. 2016

Mechanisms of Development

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“…The gastrointestinal system of postnatal marsupials has been explored, aiding to tell the story of the evolution of this system in vertebrates [96][97][98][99]. Studies on marsupial pouch joeys have shown that every phase of milk has specific factors that trigger phenotypic changes in the neonate stomach [100], and that the milk regulates the microbiota in the neonates' intestine [101].…”

Section: The Main Tissues and Organs In Development In The White-earementioning

confidence: 99%

Didelphis albiventris: an overview of an unprecedented transcriptome sequencing of the white-eared opossum

Santos

Mendes

Silva

et al. 2019

Preprint

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Background The white-eared opossum (Didelphis albiventris) is widely distributed throughout Brazil and South America. They have been used as animal models to study different aspects in science, from the restoration of degraded green areas to medical aspects of the Chagas disease, leishmaniasis and resistance against snake venom. As a marsupial, D. albiventris can also aid the comprehension of the molecular mechanisms that govern the different stages of organogenesis, as their joeys are born after only 13 days, depending on placentation, and the final stages of organogenesis occurs when the neonates are inside the pouch, depending on lactation. As the genome of this opossum species, and/or its transcriptome, had not been completely sequenced yet, the use of D. albiventris as an animal model is limited. In this work, we have sequenced the D. albiventris transcriptome, by RNA-seq, to obtain the first catalogue of differentially expressed genes (DE) and gene ontology (GO) annotation during the neonatal stages of the marsupial development. Results The D. albiventris transcriptome was obtained from whole neonates harvested at birth (P0), at five days old (P5) and ten days old (P10). The de novo assembly of these transcripts allowed us to obtain 85,338 transcripts. Only ~30% of these transcripts could be mapped against M. domestica genome, the closest phylogenetic relative with available nucleotide sequences. Among the expressed transcripts, 2,077 were found to be DE between P0 and P5, 13,780 between P0 and P10, and 1,453 between P5 and P10. Enriched GO terms were mainly related to immune system, blood tissue development and differentiation, vision, hearing, digestion, CNS and limb development. Conclusions The unveiling of opossum transcriptomes provides an out-group to better understand the distinct characteristics associated with the evolution of mammalian species. Nevertheless, this is the first transcriptome sequencing and available catalogue of genes of a marsupial species at different neonatal stages, allowing the study of mechanisms involved in the organogenesis.

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“…From an evolutionary perspective, its composition has evolved over time in order to provide the infant a well-balanced nutrition and protection against potential infectious pathogens while the neonatal immune system completes its development. 3 Influences on compositional differences include time of lactation, length of gestation, maternal diseases, genotype and diet. 4 The mean protein content gradually decreases from the second to the sixth-seventh month of lactation and stabilizes thereafter.…”

Section: Introductionmentioning

confidence: 99%

Human milk: composition and health benefits

2017

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Breastfeeding is widely acknowledged as the normal and unequalled method for feeding infants due to its associated health benefits, both for the infant and the mother. The World Health Organization recommends that infants are exclusively breastfed up to the completion of six months of age, with breastfeeding continuing to be an important part of the diet until the infant is at least two years old. The several health benefits associated with breastfeeding are driven by the combined action of the nutritional and bioactive components in human milk and the magnitude of the majority of the ascertained biological effects is directly dependent on breastfeeding duration. This review briefly summarizes the current knowledge on the composition of human milk and provides an overview on its functional effects on health outcomes, focusing on the latest research results.

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Bioactive Functions of Milk Proteins: a Comparative Genomics Approach

Cited by 28 publications

References 144 publications

Marsupial tammar wallaby delivers milk bioactives to altricial pouch young to support lung development

Marsupial tammar wallaby delivers milk bioactives to altricial pouch young to support lung development

Didelphis albiventris: an overview of an unprecedented transcriptome sequencing of the white-eared opossum

Human milk: composition and health benefits

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