Bioactive flavonoid from the Commelina obliqua Vahl. and its In vitro anti-inflammatory activity

Abstract

<em>Commelina obliqua</em> Vahl. This plant is a member of the Commelinaceae family, commonly referred to as the dayflower family. An entirely new bioactive compound recently discovered and identified 3, 5, 7, 3' 4′-pentahydroxy flavone-7-O-L-ribopyranosyl-3′-O-D-galactopyranoside having a melting point 320-22<sup>°</sup>C, molecular formula C<sub>26</sub>H<sub>28</sub>O<sub>16</sub>, m/z [595-H]<sup>-</sup> ESI-MS negative mode by different chemical color reactions, chemical decomposition, and spectral analysis 1HNMR, 13CNMR, Electron spray ionization mass spectrometry, Fourier transform infrared spectroscopy. An<em> in vitro </em>anti-inflammatory test revealed significant anti-inflammatory effects besides the degradation of egg albumin. Compound M illustrated the more percentage inhibitory action (123.1974%) of protein denaturation at 500 μg/ml, and Diclofenac sodium demonstrated less inhibitory activity (120.0627%) at the equivalent combination. As a result, Compound M inhibits protein denaturation more effectively than Diclofenac sodium at the same combination. Therefore, compound M may utilize as an anti-inflammatory source.