Bioactive extracts and chemical constituents of two endophytic strains of Fusarium oxysporum

Abstract

Abstract:Ethyl acetate extracts of cultures grown in liquid Czapek and on solid rice media of the fungal endophyte Fusarium oxysporum SS46 isolated from the medicinal plant Smallanthus sonchifolius (Poepp.) H. Rob., Asteraceae, exhibited considerable cytotoxic activity when tested in vitro against human cancer cells. Chromatographic separation yielded anhydrofusarubin (1) and beauvericin (2) that were identified based on their 1 H and 13 C NMR data. Compounds 1 and 2 showed the strongest cytotoxic activity against different cancer cell lines. Compound 2 also showed promising activity against Leishmania braziliensis. Hexanic extract of F. oxysporum SS50 grown on solid rice media also afforded a mixture of compounds that displayed cytotoxic activity against different cancer cell lines. Chemical analysis of the mixture of compounds, investigated by gas chromatography-mass spectrometry (GC-MS), showed that there was a predominance of methyl esters of fatty acids and alkanes.

Distribution, Ethnobotany, Pharmacognosy and Phytoconstituents of Coptis teeta Wall.: A Highly Valued and Threatened Medicinal Plant of Eastern Himalayas

“…22 Antimicrobial Anti-inflammatory 23 likewise Methyl Streate has already been reported as antidiarrheal. 25 Therefore, it is concluded that Coptis teeta need protection, propagation and further studies for the welfare of ecosystem and mankind.…”

Antimicrobial activity of metabolites extracted from Zanthoxylum gilletii, Markhamia lutea and their endophytic fungi against common bean bacterial pathogens

“…Fermentation of the endophyte was carried out using a procedure by Nascimento et al (2012) with slight modification. The solid-state fermentation was carried out in 21, 500 ml Erlenmeyer flasks containing 90 g of rice in 90 ml of distilled water per flask which were twice autoclaved at 120°C for 40 min.…”

Direct MALDI-TOF/TOF analyses of unnatural beauvericins produced by the endophytic fungus Fusarium oxysporum SS46

“…The resulting mycelia were extracted with 300 ml of methanol for 48 h to yield the crude mycelial extracts. The production of natural beauvericin was analyzed by high performance liquid chromatography coupled with a photodiode array detector (HPLC-PDA), by comparison with a previously isolated beauvericin standard (Nascimento et al, 2012). HPLC analyses were carried out by a Shimadzu ® HPLC with LC-6AD pumps, a SCL-10AVP system controller, a diode array detector UV/VIS SPD-M10AVP and RID-10A, SIL-10AF autosampler, and Class VP software.…”

“…The endophytic fungus Fusarium oxysporum SS46, isolated from the host plant Smallanthus sonchifolius (Gallo et al, 2009), is a producer of the cyclohexadepsipeptide beauvericin (Nascimento et al, 2012). Beauvericin has antibiotic, antifungal, insecticidal, antimycobacterial, antiplasmodial, and nematicidal properties, and also inhibits angiogenesis, cell migration, and cancer cell proliferation (Xu et al, 2007;Zhan et al, 2007;Zhang et al, 2007;Xu et al, 2009;Shimada et al, 2010).…”

“…Beauvericin has antibiotic, antifungal, insecticidal, antimycobacterial, antiplasmodial, and nematicidal properties, and also inhibits angiogenesis, cell migration, and cancer cell proliferation (Xu et al, 2007;Zhan et al, 2007;Zhang et al, 2007;Xu et al, 2009;Shimada et al, 2010). Recently, beauvericin displayed promising activity against Leishmania braziliensis (Nascimento et al, 2012). Thus, this compound has interesting pharmacological properties that suggest its potential as a candidate for drug development (Tedjiotsop Feudjio et al, 2010).…”