Bioactive Constituents of Shoot Extracts of Sisymbrium irio L. Against Fusarium oxysporum f. sp. cepae

Abstract

The present study was carried out to check the antifungal potential of Sisymbrium irio L. shoot extract against Fusarium oxysporum f. sp. cepae (FOC). In preliminary bioassays, different concentrations (1 to 5%) of leaf, stem and fruit extracts were evaluated against FOC. All the extracts were effective against the pathogen. However, the leaf extract was found the most effective causing 25-41% decrease in FOC biomass. The fractionation of methanolic leaf extract was done by two organic solvents namely n-hexane and chloroform. Different concentrations (1.56 to 200 mg mL-1) of these fractions were tested against FOC. The n-hexane and chloroform fractions showed inhibitory activity against the pathogen and resulted in 77-93% and 80-96% reduction in biomass of FOC, respectively. GC-MS analysis showed the presence of 24 compounds in n-hexane and 4 compounds in chloroform fraction. In n-hexane fraction, β-sitosterol (18.64%) was the most abundant compound followed by orotic acid, bis(tert-butyldimethylsilyl)-, tert-butyldimethylsilyl ester (12.18%), 10-octadecenoic acid, methyl ester (7.90%) and 1,2-benzenedicarboxylic acid, diisooctyl ester (6.05%). Major compounds identified in chloroform fraction were 1,3-benzenedicarboxylic acid, bis(2-ethylhexyl) ester (50.82%) and di-n-octyl phthalate (33.00%). This study concludes that n-hexane and chloroform fractions of methanolic leaf extract of S. irio contain potent antifungal constituents for the management of FOC.

The Effect of Supplementing Mushroom Growing Substrates on the Bioactive Compounds, Antimicrobial Activity, and Antioxidant Activity of Pleurotus ostreatus

“…ostreatus mushroom. Compounds such as heptadecane, hexadecane, pentadecane, dibutyl phthalate, cholest-22-ene-21-ol, 3,5-dehydro-6-methoxy- and ß-sitosterol which were detected on some of the Pleurotus ostreatus mushrooms are well known to have antimicrobial activity [ 46 – 51 ]. Such findings prove that some of the activities observed such antioxidant and antimicrobial were mainly due to the presence of the above-mentioned compounds.…”

Identification of compounds present in lipophilic extracts from Brachystola magna (Girard): substances with potential healing properties

“…Furthermore, it has been reported that hexatriacontane possess antioxidant (Taha et al 2021) and antimicrobial (Afzal et al 2014) activities; heptadecanoic acid, 16-methyl-, methyl ester has exhibited antibacterial (Vijayakumar et al 2021) and antioxidant (Fatema et al 2019) activities as well as properties against skin cancer (Kandasamy et al 2012); oxalic acid, isobutyl nonyl ester has showed antibacterial (Tapfuma et al 2020), antioxidant, anti-inflammatory and anticancer (Chinnasamy et al 2018) activities; 1-propene-1,2,3-tricarboxylic acid, tributyl ester has been studied as an antioxidant (Xuan et al 2018) and as an agent for gastrointestinal infections (Theresa Ibibia et al 2016); 1,3-benzenedicarboxylic acid, bis (2-ethylhexyl) ester has shown antibacterial, antifungal (Akhtar et al 2020) and anti-inflammatory (Winner et al 2016) properties.…”

In vitro efficacy of biologically active compounds derived from Navicula arenaria against soil borne phytopathogenic Macrophomina phaseolina and Fusarium oxysporum

“…6). Some of them were recorded as antifungal compounds like phenol, 2,2'-methylenebis [6-(1,1dimethylethyl)-4-methyl- [26], di-n-octyl phthalate [27], beta.-Sitostero [27], cholestan-3-ol, (3.beta.,5.beta. )- [28] and Cholestane-3,5-diol, 5-acetate, (3.beta.,5.alpha.…”