Bioactive constituents of germinated fenugreek seeds with strong antioxidant potential

Khole, Swati; Chatterjee, Suchandra; Variyar, Prasad S.; Sharma, Arun; Devasagayam, T.P.A.; Ghaskadbi, Surendra

doi:10.1016/j.jff.2013.10.016

Cited by 79 publications

(61 citation statements)

References 34 publications

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“…This observation can be correlated to increased total polyphenolic, flavonoid, and sapogenin contents in germinated seeds (Table ). Flavonoids such as isovitexin and vitexin are known to increase on germination of fenugreek seeds (Khole et al, ). Randhir, Lin, and Shetty () reported higher antioxidant activity during early germination which correlated with higher phenolic content.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of debittered and germinated fenugreek (Trigonella foenum graecumL.) seed flour on the chemical characteristics, biological activities, and sensory profile of fortified bread

Chaubey

Somani

Kanchan

et al. 2017

J Food Process Preserv

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Germination of fenugreek seed showed better nutraceutical profile as evaluated by in vivo (oral glucose tolerance and acute antihypertriglyceridemic tests) on rats and in vitro DPPH and enzyme inhibition assays (α‐glucosidase, lipase, and lipoxygenase). Attempts were made to develop debittered and germinated fenugreek seed flour (DGFSF) by soaking in acesulfame (0.25%, 8 hr), curd (1:1 in water, 8 hr) and sucrose (5%, 8 hr) solution at 1:4 (wt/vol) ratio for debittering and then germinated, of which curd showed the maximum extent of debittering. The effect of substitution of wheat flour with 5, 10, 15, and 20% (wt/wt) of DGFSF on chemical characteristics and sensory properties was investigated; 10% fortified bread was organoleptically acceptable and had enhanced protein and fiber contents. Fortification increased the bulk density and crumb firmness, decreased the specific loaf volume, and darkened the crumb color. Glycemic index of the bread decreased with 10% DGFSF, which is indicative of desirable health benefits. Practical applications Fenugreek seeds are major constituent of Indian spices, bitter to taste and possess various medicinal properties. These seeds are an excellent source of dietary fiber and protein. Although incorporated into traditional foods, its bitterness limits its wide usage. In India, soaking and germination of seeds in water or curd was practiced traditionally since ancient days. Germination and debittering improved antioxidant activity, and various in vitro and in vivo bioactivities. Incorporation of debittered flour improved the polyphenolic, protein, and dietary fiber contents in bread which in turn demonstrated reduced rate of in vitro starch digestibility or lower glycemic index

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Section: Resultsmentioning

confidence: 99%

Evaluation of debittered and germinated fenugreek (Trigonella foenum graecumL.) seed flour on the chemical characteristics, biological activities, and sensory profile of fortified bread

Chaubey

Somani

Kanchan

et al. 2017

J Food Process Preserv

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“…6 Bioac tivity guided isolation of active antioxidant compounds from germinated fenugreek seeds revealed apigenin8Cβglucopyranoside (vitexin) and apigenin6Cβglucopyranoside (isovitexin) as major compounds. 7 Both these compounds have received critical attention due to their antioxidant and antidiabetic properties. Isovitexin is known to inhibit xanthine oxidase activity and protect DNA against cellular ROS.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, from all the obtained data it's evident that besides the free hydroxyl group in the B ring of apigenin, substitution of glucose at the C6 and C8 position of the A rings also plays a role in antioxidant activity. Both these compounds form a major component of germinated fenugreek seeds (16.84 and 17.25 mg/kg of germinated fenugreek seeds of vitexin and isovitexin respectively) 7 and hence could together contribute effectively to the antioxidant activity of fenugreek seeds.…”

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confidence: 99%

Comprehensive Assessment of Antioxidant Activities of Apigenin Isomers: Vitexin and Isovitexin

Khole

Panat

Suryawanshi

et al. 2016

FRA

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Introduction: Fenugreek is known to possess anti-diabetic as well as antioxidant properties known to increase with germination. Previous study from our laboratory has reported vitexin and isovitexin as major antioxidant compounds from germinated fenugreek seeds. The present study aimed to comprehensively assesses and compare their antioxidant activity. Methods: The antioxidant ability of these compounds against various radicals was checked using standard in vitro radical scavenging assays and pulse radiolysis. Protection conferred to mitochondria against oxidative damage was assessed by measuring the levels of lipid peroxides and protein sulphydryls. Protection from hydrogen peroxide induced cytotoxicity in HepG2 cells was checked by MTT assay and by measuring intracellular ROS. Their ability to modulate intracellular antioxidant status was checked both at activity and RNA level. Result: Vitexin and isovitexin exhibited differential antioxidant activity against various radicals. Vitexin was a better nitric oxide scavenger while isovitexin scavenged superoxide radicals more efficiently. Pulse radiolysis studies revealed good antioxidant activity of both compounds against short-lived radicals and affected the formation and decay of the 2-dG transient species to the same extent. In oxidatively damaged mitochondria, lipid peroxidation was inhibited significantly by isovitexin whereas vitexin prevented decrease in protein sulphydryl content more effectively. These compounds protected HepG2 cells to the same extent against hydrogen peroxide induced oxidative insult by reducing intracellular ROS and modulating the levels of antioxidant enzymes. Conclusion: The present study clearly demonstrates differential antioxidant potential of both vitexin and isovitexin and their role in mitigating oxidative stress induced damage and maintaining cellular redox homeostasis.

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“…Reported biological activities include anti-cancer [11][12][13][14][15][16], anti-diabetic [17], anti-obesity [18], hypocholesterolaemic [19], antiplasmodial [20], antioxidant [21], anti-inflammatory, antipyretic and antinociceptive activities [22,23] as well as inhibition of hyperpigmentation [14] and inhibition of the enzyme acetyl cholinesterase [24], a biological activity of potential significance in the treatment of memory disorders associated with Alzheimer's disease. Also of interest are biological activities bestowed by germination of the seeds, such as increased antioxidant potential [25]. Furthermore, even a plant-plant related allelopathic biological activity that of phytotoxicity to lettuce seeed germination and growth was reported for Fenugreek seeds [26].…”

Section: Introductionmentioning

confidence: 99%

Variability in the Content of 25α- and 25β-Steroidal Sapogenins among Thirty Morphologically Different Sudanese Seed Accessions of Fenugreek

Osman¹,

Elhussein²,

Albalawi³

2014

JLS

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Abstract:Seeds of Fenugreek (Trigonellafoenum-graecum L.), an international spice commodity, are much consumed in Sudan as a food, for flavoring and as a folk-remedy for several ailments, together with local beliefs in nutraceutical benefits. The authors have noted an immense variability in colour and other morphological characters of Fenugreek seeds offered in local Sudanese markets and wondered whether this variability is extended to their chemical composition. Steroidal sapogenins are important chemical constituents of Fenugreek seeds in view of their potential as precursors for the commercial synthesis of steroid drugs and their continually revealed beneficial biological activities. In this study, thirty Sudanese Fenugreek seed accessions collected from different geographical regions in Sudan were analyzed for their 25α-, 25β-and total sapogenin content using a simple and specific infra-red spectroscopic method. The seed accessions exhibited much morphological variability particularly in outer seed coat color and size. Preparative TLC followed by gravimetric analysis showed that steroidal sapogenins, mostly diosgenin and yamogenin, represented more than 70% of the steroids of Fenugreek seeds. Infra-red spectroscopic analysis showed that total 25α-sapogenins (calculated as diosgenin) varied from 0.65% to 1.68%. Total 25β-sapogenin (calculated as yamogenin) varied from 0.38% to 2.03%. The content of total sapogenins (α + β epimeric forms) varied between 1.24% and 3.0% of the oven-dry weight of seeds. β-sapogenins (mostly yamogenin) were dominant over α-sapogenins (diosgenin) in most Sudanese Fenugreek seed accessions.

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Bioactive constituents of germinated fenugreek seeds with strong antioxidant potential

Cited by 79 publications

References 34 publications

Evaluation of debittered and germinated fenugreek (Trigonella foenum graecumL.) seed flour on the chemical characteristics, biological activities, and sensory profile of fortified bread

Evaluation of debittered and germinated fenugreek (Trigonella foenum graecumL.) seed flour on the chemical characteristics, biological activities, and sensory profile of fortified bread

Comprehensive Assessment of Antioxidant Activities of Apigenin Isomers: Vitexin and Isovitexin

Variability in the Content of 25α- and 25β-Steroidal Sapogenins among Thirty Morphologically Different Sudanese Seed Accessions of Fenugreek

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