Bioactive constituents from the bacteirium Alcaligenes faecalis YMF 3.175

Xu, Y.Y.; Wang, X.; Zhang, K. Q.; Li, G. H.

doi:10.1134/s0003683815010172

Cited by 6 publications

(3 citation statements)

References 20 publications

(13 reference statements)

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“…The BCA is reportedly adaptive to harsh environmental conditions and encodes HCN, siderophore, and phenolic compound synthesis, exhibiting mortality against nematodes ( Felestrino et al, 2020 ). The dominance of chitin in nematode eggs and breakthrough for genes encoding chitinolytic, esterase activity, proteases and peptides ( Annamalai et al, 2011 ), and production of dimethyl sulfate recognizes the strain as a future promising antinemic microorganism ( Xu et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicola

Jena,

Choudhury,

Das

et al. 2023

PeerJ

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Meloidogyne graminicola has a well-established negative impact on rice yield in transplanted and direct-seeded rice, resulting in yield losses of up to 20 to 90 percent. Studies were undertaken to isolate potential native strains of bio-control agents to manage the devastating Rice Root Knot Nematode (M. graminicola). Eighteen bacterial strains and eleven fungal strains were isolated from the rhizosphere of crops like rice, okra, ash gourd, chili, beans and cucumber, enveloping diverse soil types from the Upper Brahmaputra Valley region of Assam. Six bacterial strains were gram-positive according to morphological results, while twelve others stained negatively. Fifteen bacteria were rod-shaped, two were coccus and one was diplococcus, and all the bacterial isolates showed signs of movement. All the bacterial strains exhibited positivity for gelatin hydrolysis and catalase test. Seven bacteria showed positive, while eleven showed negative reactions to possess the ability to deduce carbon and energy from citrate. The study of the in vitro efficacy of the twenty-nine bacterial and fungal isolates tested against second-stage juveniles (J2) of Meloidogyne graminicola revealed that all the bacterial and fungal isolates potentially inhibited the test organism and caused significant mortality over sterile water treatment. The promising bacterial and fungal isolates that exhibited mortality above 50% were identified as BSH8, BTS4, BTS5, BJA15, FJB 11 and FSH5. The strain BSH8 exhibited the best result of mortality, with 80.79% mortality against J2 of M. graminicola. The strain BTS4 and BTS5 expressed mortality of 71.29% and 68.75% under in-vitro conditions and were significant. The effective and promising bioagents were identified using the 16 S rRNA sequencing as Bacillis subtilis (BSH8), Bacillus velezensis (BTS4), Alcaligenes faecalis (BTS5), Rhizobium pusense (BJA15), Talaromyces allahabadensis (FSH5) and Trichoderma asperellum (FJB11). These results indicated the microorganism’s potential against M. graminicola and its potential for successful biological implementation. Further, the native strains could be tested against various nematode pests of rice in field conditions. Its compatibility with various pesticides and the implication of the potential strains in integrated pest management can be assessed.

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Section: Discussionmentioning

confidence: 99%

Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicola

Jena,

Choudhury,

Das

et al. 2023

PeerJ

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“…Additionally, A7 was shown to produce several interesting bioactive compounds with known and novel activities. According to earlier research, lipid derivatives such as 9-octadecanoic acid, hexadecanoic acid methyl ester, octadecenoic acid, 1,2-benzene dicarboxylic acid, mono (2-Ethylhexyl) ester, 1,2-benzene dicarboxylic acid and diisooctyl ester [ 159 ] were found to be powerful antibacterial compounds [ 103 ]. They induced the autolysis of cell membranes, damaged cell walls, and inhibited transcription, and translation in S. aureus , E. coli , and Salmonella sp.…”

Section: Discussionmentioning

confidence: 99%

An Evaluation of Aluminum Tolerant Pseudomonas aeruginosa A7 for In Vivo Suppression of Fusarium Wilt of Chickpea Caused by Fusarium oxysporum f. sp. ciceris and Growth Promotion of Chickpea

et al. 2022

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Chickpea wilt, caused by Fusarium oxysporum f. sp. ciceris, is a disease that decreases chickpea productivity and quality and can reduce its yield by as much as 15%. A newly isolated, moss rhizoid-associated Pseudomonas aeruginosa strain A7, demonstrated strong inhibition of Fusarium oxysporum f. sp. ciceris growth. An in vitro antimicrobial assay revealed A7 to suppress the growth of several fungal and bacterial plant pathogens by secreting secondary metabolites and by producing volatile compounds. In an in vivo pot experiment with Fusarium wilt infection in chickpea, the antagonist A7 exhibited a disease reduction by 77 ± 1.5%, and significantly reduced the disease incidence and severity indexes. Furthermore, A7 promoted chickpea growth in terms of root and shoot length and dry biomass during pot assay. The strain exhibited several traits associated with plant growth promotion, extracellular enzymatic production, and stress tolerance. Under aluminum stress conditions, in vitro growth of chickpea plants by A7 resulted in a significant increase in root length and plant biomass production. Additionally, hallmark genes for antibiotics production were identified in A7. The methanol extract of strain A7 demonstrated antimicrobial activity, leading to the identification of various antimicrobial compounds based on retention time and molecular weight. These findings strongly suggest that the strain’s significant biocontrol potential and plant growth enhancement could be a potential environmentally friendly process in agricultural crop production.

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“…The plant growth-promoting substances included hydrolytic enzymes, hydrogencyanide, phenolics, antioxidant substances, phytohormones, IAA, gibberellic acid, transzeatin ribosides, abscisic acid, ammonia, and phosphatise [32]. A. faecalis can produce maleic acid cis-trans isomerase, nicotinic acid, picolinic acid [33], abundant antifungal volatiles against F. graminearum, F. equiseti, Alternaria alternata, Botrytis cinerea, Aspergillus niger, and Colletotrichum graminicola [34], 1,2-benzenedicarboxylic acid bis (2α-methylheptyl) ester, cyclo (L-Pro-L-Val), cyclo (Gly-L-Pro), 3-pyridinecarboxylic acid, cyclo(L-Pro-L-Tyr), adenosine and L-Val [35], succinoglucan and exocellular acidic polysaccharide [36], and nitrilase [37]. Herein, this study aims to (1) isolate and identify bacterial endophytes from Moringa oleifera leaves, (2) determine phytochemicals and bioactive compounds using GC-MS, and (3) assess their antimicrobial, antioxidant, and anticancer activities.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Endophytes from Moringa oleifera Leaves as a Promising Source for Bioactive Compounds

et al. 2023

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Bacterial endophytes reside within the tissues of living plant species without causing any harm or disease to their hosts. Bacterial endophytes have produced a variety of bioactive compounds that can be used for different biomedical applications. In the current study, two bacterial endophytes were isolated from healthy Moringa oleifera leaves, and identified genetically as Stenotrophomonas maltophilia and Alcaligenes faecalis. Phytochemical results illustrated that A. faecalis produced phenolics at 547.2 mg/g, tannins at 156.7 µg/g, flavonoids at 32.8 µg/g, and alkaloids at 111.2 µg/g compared to S. maltophilia, which produced phenolics at 299.5 mg/g, tannins at 78.2 µg/g, flavonoids at 12.4 µg/g, and alkaloids at 29.4 µg/g. GC-MS analysis indicated that A. faecalis extract has 24 bioactive compounds, including 9 major compounds, namely octadecanoic acid, hexadecanoic acid, linoleic acid ethyl ester, octadecenoic acid, methyl ester, methyl stearate, nonacosane, indolizine, palmitoleic acid, and heptacosane. On the other hand, S. maltophilia extract has 11 bioactive compounds, including 8 major compounds, namely oleic acid, octadecanoic acid, hexadecanoic acid, cis-2-phenyl-1, 3-dioxolane-4-methyl, ergotamine, diisooctyl phthalate, diethyl phthalate, and pentadecanoic acid. To check the safety of these extracts, the cytotoxicity of Ethyl acetate (EA) extracts of S. maltophilia and A. faecalis were evaluated against the Vero normal cell line, and the results confirmed that these extracts are safe to use. Moreover, results revealed that EA extracts of S. maltophilia and A. faecalis exhibited anticancer activity against the cancerous MCF7 cell line, where IC50 was 202.4 and 119.7 µg/mL, respectively. Furthermore, EA extracts of S. maltophilia had antibacterial and antifungal activity against Gram-positive and Gram-negative bacteria, and unicellular fungi. Likewise, the EA extract of A. faecalis exhibited antibacterial and antifungal activity against Gram-positive bacteria, as well as unicellular fungi, but did not show any activity against Gram-negative bacteria. Also, EA extracts of S. maltophilia and A. faecalis exhibited moderate antioxidant activity where IC50 were 146.2 and 147.6 µg/mL, respectively. In conclusion, the two isolated endophytic bacteria S. maltophilia and A. faecalis have promising bioactive compounds that have antibacterial, antioxidant, and anticancer activities.

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Bioactive constituents from the bacteirium Alcaligenes faecalis YMF 3.175

Cited by 6 publications

References 20 publications

Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicola

Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicola

An Evaluation of Aluminum Tolerant Pseudomonas aeruginosa A7 for In Vivo Suppression of Fusarium Wilt of Chickpea Caused by Fusarium oxysporum f. sp. ciceris and Growth Promotion of Chickpea

Bacterial Endophytes from Moringa oleifera Leaves as a Promising Source for Bioactive Compounds

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