Bioactive Compounds of Ziziphus spina-christi Seeds Extract and Cellulase Enzyme Attenuates the Growth of Acanthamoeba polyphaga Isolated From Contact Lenses

Abdel-Hakeem, Sara S.; Omar, Hossam El- din; Abed, Gamal Hassan; Hassan, Faten; Al‐Bedak, Osama A.; Tolba, Mohammed Essa Marghany

doi:10.21608/eajbsz.2021.210632

Cited by 2 publications

(2 citation statements)

References 27 publications

(35 reference statements)

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“…net), identified by its retention indices (relative to n-alkanes C8-C22) and mass spectrum matching to available authentic standards. Wiley spectral library collection and the National Institute of Standards and Technology (NIST) library database curves generated by running GC analysis of representative authentic compounds 24,27 .…”

Section: Gas Chromatography-mass Spectrometry (Gc-ms) Analysis Of P P...mentioning

confidence: 99%

“…All experiments were conducted in triplicates and statistical analyses were performed to determine the cell viability percentage. Furthermore, the results of the cultures containing no viable cysts were confirmed by inoculating onto an NNA-E. coli plate at 30 °C for an additional three days and examining for any viable trophozoites or cysts 24 .…”

Section: Amoebicidal Activity Of the Extract Against Trophozoites And...mentioning

confidence: 99%

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Combating the causative agent of amoebic keratitis, Acanthamoeba castellanii, using Padina pavonica alcoholic extract: toxicokinetic and molecular docking approaches

Abdel-Hakeem,

Hassan,

Hifney

et al. 2024

Sci Rep

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Natural products play a significant role in providing the current demand as antiparasitic agents, which offer an attractive approach for the discovery of novel drugs. The present study aimed to evaluate in vitro the potential impact of seaweed Padina pavonica (P. pavonica) extract in combating Acanthamoeba castellanii (A. castellanii). The phytochemical constituents of the extract were characterized by Gas chromatography–mass spectrometry. Six concentrations of the algal extract were used to evaluate its antiprotozoal activity at various incubation periods. Our results showed that the extract has significant inhibition against trophozoites and cysts viability, with complete inhibition at the high concentrations. The IC50 of P. pavonica extract was 4.56 and 4.89 µg/mL for trophozoites and cysts, respectively, at 24 h. Morphological alterations of A. castellanii trophozoites/cysts treated with the extract were assessed using inverted and scanning electron microscopes and showed severe damage features upon treatment with the extract at different concentrations. Molecular Docking of extracted compounds against Acanthamoeba cytochrome P450 monooxygenase (AcCYP51) was performed using Autodock vina1.5.6. A pharmacokinetic study using SwissADME was also conducted to investigate the potentiality of the identified bioactive compounds from Padina extract to be orally active drug candidates. In conclusion, this study highlights the in vitro amoebicidal activity of P. pavonica extract against A. castellanii adults and cysts and suggests potential AcCYP51 inhibition.

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Section: Gas Chromatography-mass Spectrometry (Gc-ms) Analysis Of P P...mentioning

confidence: 99%

Section: Amoebicidal Activity Of the Extract Against Trophozoites And...mentioning

confidence: 99%

Combating the causative agent of amoebic keratitis, Acanthamoeba castellanii, using Padina pavonica alcoholic extract: toxicokinetic and molecular docking approaches

Abdel-Hakeem,

Hassan,

Hifney

et al. 2024

Sci Rep

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Assessment of cattle tick infestation: Molecular insights into Rhipicephalus annulatus and the efficacy of garlic oil and nanoemulsion as acaricidal agents

Abd-Elrahman,

Dyab,

Kamel

et al. 2024

Veterinary Parasitology

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Bioactive Compounds of Ziziphus spina-christi Seeds Extract and Cellulase Enzyme Attenuates the Growth of Acanthamoeba polyphaga Isolated From Contact Lenses

Abstract: http://eajbsz.journals.ekb.eg/ Bioactive Compounds of Ziziphus spina-christi Seeds Extract and Cellulase Enzyme Attenuates the Growth of Acanthamoeba polyphaga Isolated From Contact Lenses

Cited by 2 publications

References 27 publications

Combating the causative agent of amoebic keratitis, Acanthamoeba castellanii, using Padina pavonica alcoholic extract: toxicokinetic and molecular docking approaches

Combating the causative agent of amoebic keratitis, Acanthamoeba castellanii, using Padina pavonica alcoholic extract: toxicokinetic and molecular docking approaches

Assessment of cattle tick infestation: Molecular insights into Rhipicephalus annulatus and the efficacy of garlic oil and nanoemulsion as acaricidal agents

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